Exogenous lactate is essential for metabolic activities in isolated rat spermatocytes and spermatids.

Spermatocytes and round spermatids were isolated from rat testis and the effects of addition of 3.3 mM-glucose and 6 mM-DL-lactate to the incubation medium on the morphology, oxygen consumption and incorporation of uridine and amino acids of these cells were investigated. Addition of lactate to isolated germ cells increased O2 consumption 1.

[The development of phlebology in the last 30 years].

Phlebology originated in Paris and Tübingen where Jean Sicard and Paul Linser accidentally discovered the possibility of the sclerotherapy of varicose veins. The second element in the treatment of lymphovenous insufficiency is ambulatory compression described as far back as the 18th century. It is only recently that reasons for its efficiency have been understood, with the study of fibrinolysis (which is counter-thrombotic), in England.

Oestrogen receptors in rat mammary tissue and plasma concentrations of prolactin during mammary carcinogenesis induced by oestrogen and ionizing radiation.

Female-Sprague-Dawley rats received a subcutaneous implant containing 2 mg oestradiol at the age of 7 weeks. One week later half of the rats treated with oestrogen and half of the rats in an untreated control group were irradiated with 2 Gy (200 rad) of X-rays. The content of oestrogen receptor of the mammary tissue and the concentration of prolactin in the plasma were studied at intervals of 2 months for a period of 14 months after this treatment.

The use of affinity matrices in the purification of inhibin from bovine follicular fluid.

The protein associated with inhibin-like activity in bovine follicular fluid was purified 80- to 120-fold after successive adsorptions on different affinity matrices, i.e. Matrex gel red A, phenyl sepharose, omega-aminohexyl agarose and concanavalin-A sepharose.

Cardioacceleratory effect of muscarinic blocking agents in the dog.

To test whether the tachycardia in response to atropine after adrenergic blockade is partly due to a central excitatory action, the effects of atropine, methylatropine and methylscopolamine were compared in dogs in neurolept-anesthesia. The latter two agents proved to have effect, similar to atropine. A central action is therefore improbable.

Nuclear androgen receptors in human prostatic tissue. Extraction with heparin and estimation of the number of binding sites with different methods.

A procedure for the estimation of nuclear androgen receptors in benign prostatic hyperplastic tissue is described, which employs extraction of receptors from nuclei with buffers containing heparin. Extraction of a nuclear pellet with a heparin-containing (1 g/l) buffer appeared to have definite advantages over 0.4 mol/l KCl extraction.

[Proctological survey in a phlebology clinic].

At a phlebological centre, the writer was in a position to see proctological patients without its being possible to establish any particular connection between varicose veins and hemorrhoids. In this paper, he examines the prevalence of proctological symptoms and specifies which treatments are applied.

[Statistical, clinical and therapeutic remarks on the external saphenous vein].

The authors review the frequency of insufficiency of the arches of the external saphenous and the development of varicose veins, and analyze the effect of sclerosis of these arches using different concentrations of Trombovar. This is a mainly short term study of 84 cases.

Vasoactive intra-arterial therapy in peripheral occlusive arterial disease (with follow-up after 6.5 years).

After a review of the history of intra-arterial treatment, our personal experience with a complex treatment of 900 cases of arteriopathy of the lower extremities is reported. The conservative treatment includes stopping smoking, plus diet, exercise, B and C vitamins, and administration of 1% xylocaine (lidocaine) in the femoral arteries. Antibiotics are added in patients with trophic lesions.

[Some clinical remarks concerning vascular dysplasias].

After a historical review and a review of classification, the author indicates the reasons which lead patients suffering from vascular dysplasias to consult a phlebologist. The clinical symptoms leading to consultation are haemorrhage, leg ulcer, varicose veins, phlebitis, and large legs. Some cases are presented of each of these clinical disturbances.

The mechanism of action of lutropin on regulator protein(s) involved in Leydig-cell steroidogenesis.

The dependence on lutropin of the synthesis of a proposed short-half-life protein regulator involved in Leydig-cell steroidogenesis was investigated. This was carried out by determining the effect of the protein-synthesis inhibitor cycloheximide, added before and during incubations with lutropin (and/or dibutyryl cyclic AMP), on the rate of testosterone production in suspensions of purified Leydig cells from adult rat testes. The Leydig cells were preincubated in Eagle's medium for 2.

Reversible interaction between androgen binding protein and testicular macromolecules causing inhibition of androgen binding activity.

Sertoli cells in culture isolated from immature rat testes secrete androgen binding protein (ABP) in the culture medium. Binding activity of ABP in concentrated medium was estimated with equilibrium dialysis against 1 nM dihydrotestosterone at 4 degrees C. The ABP protein activity was inhibited approximately 50% through addition of cytosol preparations from testis or liver, but not from brain tissue, to the concentrated culture medium; this inhibition remained constant for at least two days.

Evidence for the involvement of lutropin-independent RNA synthesis in Leydig cell steroidogenesis.

The effect of incubating purified Leydig cells in Eagle's medium and the subsequent effect of the RNA synthesis inhibitors, actinomycin D and cordycepin, on lutropin-stimulated testosterone synthesis have been investigated. The inhibiting effect was found to be inversely related to the time of preincubation; with cells preincubated for 0, 1, 2 and 3 h with Eagle's medium only, followed by 2-h incubation with lutropin with and without actinomycin D, testosterone synthesis was inhibited by 37 +/- 4, 31 +/- 3, 18 +/- 4 and 14 +/- 3% respectively (means +/- s.e.

Effects of cholesterol, hydroxycholesterols and calcium on pregnenolone production rates in mitochondrial fractions from rat testes.

The in vitro regulation of the mitochondrial conversion of cholesterol to pregnenolone in rat testis tissue has been further investigated. Pregnenolone production rates by isolated mitochondrial fractions could be stimulated by the addition of cholesterol. The stimulation was always highest in mitochondria isolated from lutropin-treated testes relative to control and cycloheximide treated testes.