Publications by authors named "Mojgan Aryaeipour"
Article Synopsis
- An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect specific IgG antibodies against Toxocara canis and Toxocara cati in patients with toxocariasis, analyzing serum samples.
- The study calculated sensitivity, specificity, and predictive values for both T. canis and T. cati, showing excellent sensitivity (100% for T. canis and 97% for T. cati) and decent specificity (82% for both).
- Five common immunoreactive bands were identified, with T. canis showing approximately twice the immune response compared to T. cati, indicating different immunological profiles in their somatic antigens.
Article Synopsis
- Fascioliasis is a parasitic infection caused by Fasciola species, affecting both humans and animals, yet current diagnostic methods remain under-validated despite advancements in treatment.
- This study aimed to compare the effectiveness of Real-Time PCR with indirect-ELISA to detect Fasciola hepatica infections, using a test group consisting of patients with known infections and others with different parasitic diseases or no infections.
- Results showed that 62.86% of the serum samples tested positive for F. hepatica, with both diagnostic methods exhibiting a high level of agreement (91.4% for ELISA and 99.2% for Real-Time PCR), confirming the reliability of these tests for diagnosing fascioliasis in humans
Article Synopsis
- - The study introduced a new, efficient method to differentiate between liver fluke species, Fasciola hepatica and F. gigantica, using multiplex PCR instead of the traditional PCR-RFLP technique.
- - Researchers collected 104 samples from Iranian slaughterhouses, identifying 76 samples as F. hepatica and 28 as F. gigantica, achieving an accuracy of 100% for multiplex PCR and 92% for PCR-RFLP.
- - The multiplex PCR method is highlighted for its convenience, cost-effectiveness, and rapid results, providing conclusive results in just 3 hours from sample collection.
Background: Identification of the larval stages of spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this study was investigate using morphological and molecular techniques.
View Article and Find Full Text PDFArticle Synopsis
- Fascioliasis is a worldwide disease caused by liver flukes, affecting livestock and humans, particularly prevalent in northern Iran, with limited studies on local species.
- The study aimed to identify different Fasciola species, including hybrids, in livestock from Golestan province using morphometric measurements and molecular techniques.
- Results showed 110 infected isolates, identifying 81 as F. hepatica and 29 as F. gigantica through one method, while 72, 26, and 12 were classified as F. hepatica, F. gigantica, and hybrids, respectively, marking the first molecular evidence of hybrid forms in ruminants.
Article Synopsis
- Researchers studied over 5,300 freshwater snails from 51 habitats around the Caspian Sea in northern Iran to identify the local snail species and check for trematode infections.
- Five families of snails were found, with Physidae being the most common; around 20% of the snails were infected with various parasites.
- The findings suggest a significant presence of snail-borne diseases in the region, prompting calls for more research and attention from policymakers to monitor these health risks.
Article Synopsis
- The study focused on assessing the genetic diversity of parasite samples from sheep, cattle, and goats in Lorestan Province, Iran, using the PCR-RFLP method and mitochondrial gene analysis.
- A total of 56 samples were analyzed, revealing that 93% belonged to one parasite species, while only 7% were identified as another, with no hybrid forms found.
- Findings indicated that mitochondrial genes provided more effective measures of genetic variability compared to ribosomal genes, with the ND1 gene being particularly useful for analyzing differences among species.
Article Synopsis
- The study compared two diagnostic methods, semi-nested PCR and indirect ELISA, for detecting human fasciolosis using 70 serum samples from suspected patients in Iran.
- Results showed that semi-nested PCR had a higher agreement rate (98.4%) compared to indirect ELISA (94.46%), with no false positives from other parasitic infections.
- The findings suggest that semi-nested PCR is a reliable confirmatory method and can be used to monitor patient treatment effectively.
Article Synopsis
- - Fasciolosis, a parasitic disease linked to consumption of contaminated food, has been reported for the first time in southwest Iran, highlighting the need for awareness in low-prevalence areas.
- - A 51-year-old woman with abdominal pain and fever was diagnosed with the disease after traveling to a region with higher rates and eating raw watercress, leading to the discovery of Fasciola gigantica in her bile duct.
- - The patient's elevated antibody levels indicate a serious infection, while her family tested negative, suggesting that travel history and dietary habits are crucial for accurate diagnosis of this disease.
Article Synopsis
- Fascioliasis is a parasitic disease found in livestock that has been identified in five confirmed cases in Lorestan Province, Iran, from January 2015 to June 2016.
- Of 1256 patients examined, 16 tested positive for the disease, with five cases confirmed through stool tests and PCR analysis, showing a mean age of 45 and common symptoms like abdominal pain and fever.
- The majority of patients reported consuming raw aquatic plants, particularly watercress, linking their infection to dietary habits, highlighting Lorestan as a developing area for fascioliasis with a need for further investigation.
Article Synopsis
- The study investigates a cyclo-zoonotic infection caused by tapeworms and aims to analyze genetic diversity using mitochondrial genome data.
- Researchers collected 52 tissue samples from patients in Iran over a span of 23 years, amplifying DNA to identify different genotypes through sequencing.
- Findings revealed that the G1 genotype was most common, while the G3 genotype (Buffalo strain) was also present, enhancing understanding of the parasite’s transmission and genetic variation in Northern Iran.
Background: The detection of species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related genotypes by restriction fragment length polymorphism PCR.
Methods: Seventy adult flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, south-east Iran (Jan-Jul 2017).
Article Synopsis
- A study was conducted in Kermanshah Province, Iran from 2014-2016 to assess the prevalence of fasciolosis in humans, livestock, and snails.
- The research involved analyzing slaughter records for animals, performing ELISA tests on 975 human serum samples, and collecting 4400 snails from various habitats.
- Results showed a 1.7% prevalence in livestock and 0.5% in humans, with no snail infections found, suggesting environmental factors like climate affect the parasite's survival and spread.
Article Synopsis
- Fascioliasis is a disease caused by two species of parasites, but no human infections had been officially reported in Iran until now.
- The case involved a 25-year-old woman who showed liver lesions and tested positive for the parasite through both blood and stool exams.
- Genetic testing confirmed the parasite's identity and she was successfully treated with a single dose of Egaten®, marking this as the first reported human case of fascioliasis from this specific parasite in Iran.
Article Synopsis
- The study aimed to determine the prevalence of hydatidosis in Alborz Province, Iran, by collecting and analyzing 680 serum samples from individuals across various health centers in 2014-15.
- Results showed a 3.4% positivity rate for hydatidosis, with higher rates found in males (4.7%) compared to females (3.1%), and a significant association with areas having a high population of dogs.
- The findings suggest that local authorities need to be vigilant regarding hydatidosis due to the presence of stray dogs and the risk of infection, especially among specific occupations and age groups.
Background: The aim of this study was the seroepidemiological survey for detecting the status of human fasciolosis in Lorestan Province, western Iran.
Methods: This cross-sectional study was conducted in 2015-16. Based on statistical estimations, 1256 serum samples were collected from different parts of Lorestan Province, western Iran, and stored at -20 °C until use.
Background: We aimed to describe morphological and morphometrical characteristics of spp. in livestock from Ardabil Province, Northwest Iran.
Methods: Forty adult flukes were collected from different definitive hosts (cattle and sheep).
Article Synopsis
- The study assessed how DNA vaccines encoding an 8-kDa subunit of antigen B (HydI) and murine interleukin 12 (IL-12) as genetic adjuvants affect immune responses in BALB/c mice.
- Mice were divided into five experimental groups and given different treatments; results showed that the group receiving both pcHydI and pcMIL12 had significantly higher lymphocyte proliferation and more IFN-γ production.
- The combination vaccine also led to increased IgG2a levels and reduced IL-4 production, indicating a stronger immune response compared to other groups.
Article Synopsis
- A study was conducted to analyze the genetic diversity of a specific organism (referred to as spp.) found in sheep, cattle, and goats in Kermanshah Province, Iran, using the PCR-RFLP method.
- The researchers employed PCR-RFLP analysis on a ribosomal gene fragment and confirmed species identification through sequencing, testing samples from 16 sheep, 28 cattle, and 4 goats.
- The findings revealed that the targeted species was present in 96% of samples, with another species only detected in two cattle, and no hybrids were found, providing insights into genetic variation in this area of Iran.
Article Synopsis
- Fascioliasis is a zoonotic disease affecting both livestock and humans, with a notable seroprevalence of 24.8% among patients in Tehran from 2008-2014.
- The study involved a serological evaluation using ELISA, gathering demographic data, symptoms, and eosinophil rates, with most seropositive cases being among males aged 20-30 years who had a history of consuming raw vegetables or unsafe water.
- Patients referred primarily from Gilan and Mazandaran provinces showed higher awareness and quicker referrals for fascioliasis compared to those from other regions in Iran, indicating a need for improved diagnosis in less alert areas.
Background: The aim of this study was to conduct a sero-epidemiological survey in Isfahan City and suburb areas, central Iran to detect the rate of human hydatidosis using ELISA test.
Methods: Overall, 635 serum samples were collected from subjects referred to different health centers in urban and rural regions of the city. Sera were analyzed using Ag-B ELISA test.
Background: Echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection caused by the larval stage of the dog taeniid tapeworm Echinococcus granulosus. Vaccination has been considered as one of the ways to prevent of hydatidosis in recent decades. The aim of this study was to construct a pcDNA3.
View Article and Find Full Text PDFBackground: The aim of this study was to detect the genotype of Fasciola spp. in Meshkin-Shahr, Ardabil Province, northwestern Iran in different hosts using PCR-RFLP.
Methods: The parasite hosts included cattle, and sheep.
Article Synopsis
- * 471 serum samples were tested using the ELISA method, resulting in 8 positive cases (1.7%), all of whom were female and had dietary links to local foods.
- * The findings suggest a potential risk of fascioliasis in Isfahan County, indicating a need for further research on associated risk factors and veterinary studies.