The effect of -derived extracellular vesicles on glucose metabolism and induction of insulin resistance in HepG2 cells.

infection has been associated with the development of insulin resistance (IR). This study aimed to examine the effect of -derived extracellular vesicles (EVs) on IR induction. EVs were derived from two strains, and characterised by transmission electron microscopy and dynamic light scattering.

The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro.

Helicobacter pylori has been recognized as a true pathogen, which is associated with various gastroduodenal diseases, and gastric adenocarcinoma. The crosstalk between H. pylori virulence factors and host autophagy remains challenging.

and Assessment of Antibacterial Activity, LPS Binding Affinity, and Toxicity of the GKY25 Peptide.

Introduction: Extensively and multi-drug resistant isolates of bacteria (MDR, XDR) have caused significant health problems and are responsible for high morbidity and mortality as well. In this critical condition, the discovery, design, or development of new antibiotics is of great concern. According to this necessity, antimicrobial peptides (AMPs) suggested as promising agents.

Evaluation of the antibacterial effect of hydroalcoholic extract of the galls of on .

Background: (Aa) plays a vital role in some destructive forms of periodontitis. While mechanical and chemical plaque control is the first step in periodontitis treatment, side effects of adjunctive chemical agents such as chlorhexidine (CHX) mouthwash have led to the application of natural alternatives with minimal side effects. Therefore, this study evaluated the antibacterial effect of the hydroalcoholic extract of (Qi) galls on Aa in vitro.

Expression analysis of miRNA-155 level in related inflammation and chronic gastritis.

Background And Objectives: , is a major etiologic agent associated with gastritis. There is more evidence of noncoding microRNAs (miRs) dysregulation in gastrointestinal diseases, including inflammation caused by Also, the classification of gastrointestinal malignancies using the miRs profile is better than the protein profile. MiRNA-155() among other miRs plays an important role in control of inflammation and gastric malignancy, so it can be remarkable prognosis marker of gastric cancer in the phase of chronic gastritis.

Fast killing kinetics, significant therapeutic index, and high stability of melittin-derived antimicrobial peptide.

The emergence of multidrug-resistant (MDR) bacteria is a major challenge for antimicrobial chemotherapy. Concerning this issue, antimicrobial peptides (AMPs) have been presented as novel promising antibiotics. Our previous de novo designed melittin-derived peptides (MDP1 and MDP2) indicated their potential as peptide drug leads.

Helicobacter pylori Infection Mediates Inflammation and Tumorigenesis-Associated Genes Through miR-155-5p: An Integrative Omics and Bioinformatics-Based Investigation.

Helicobacter pylori (H. pylori) is a major human pathogenic bacterium that survives in the gastric mucosa. The aim of this study is to evaluate the expression of the target gene network of miR-155-5p in H.

Cross-talk between immune system and microbiota in COVID-19.

Introduction: Human gut microbiota plays a crucial role in providing protective responses against pathogens, particularly by regulating immune system homeostasis. There is a reciprocal interaction between the gut and lung microbiota, called the gut-lung axis (GLA). Any alteration in the gut microbiota or their metabolites can cause immune dysregulation, which can impair the antiviral activity of the immune system against respiratory viruses such as severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2.

Prediction of Blood miRNA-mRNA Regulatory Network in Gastric Cancer.

Background: The aim of the study was to suggest a high specific and sensitive blood biomarker for early GC diagnosis.

Methods: the expression data of miRNAs and mRNAs were collected from the blood samples of the GC patients based on literature mining. Bioinformatics tools and databases (PANTHER, TargetScan, miRTarBase, miRDB, STRING, and Cytoscape) were used to predict the regulatory relationship.

Anti-proliferative effects of cell wall, cytoplasmic extract of and nisin through down-regulation of on SW480 colorectal cancer cell line.

Background And Objectives: Colorectal cancer is one of the most types of cancer. Researchers have shown that lactic acid bacteria have antitumor activity. The cell wall of , as the bacterial cytoplasmic extract and nisin can affect the proliferation of cancer cells.

Prevalence, serotyping and drug susceptibility patterns of isolates from kidney transplanted patients with urinary tract infections.

Background: Extended-spectrum β-lactamase (ESBL)-producing () are among the main pathogens in urinary tract infections (UTIs) among kidney transplant patients (KTPs).

Aim: To estimate the prevalence of ESBL-producing in KTPs and to evaluate the most prevalent serotypes and antibacterial susceptibility patterns of isolated bacteria in Tehran, Iran.

Methods: A total of 60 clinical isolates of uropathogenic were collected from 3 kidney transplant centers from April to May 2019.

Phenotypic Investigation of the Antimicrobial Effect of Organic and Hydro-Alcoholic Extracts of on Oral Microbiota.

Objectives: Considering the emergence of resistant microbes and side effects of chemical drugs, in this study, the inhibitory effect of organic and hydro-alcoholic extracts of () on some oral microbiota was investigated.

Materials And Methods: In this experimental study, standard strains of (; PTCC 5027), (; PTCC 5295), (; PTCC 5297), and (; PTCC 1688) were collected from the Iranian Research Organization for Science and Technology (IROST). Then, the minimum inhibitory concentration (MIC) of organic and hydro-alcoholic extracts of was determined based on the CLSI protocol and using the micro-dilution method.

Blood culture and antimicrobial susceptibility pattern of bacteria and fungi isolated from febrile neutropenic patients treated with chemotherapy at Taleghani hospital, Tehran.

Background And Objectives: The aim of this study was to determine the drug susceptibility pattern of the pathogens causing bacteraemia and fungemia in patients who have developed febrile neutropenia after chemotherapy.

Materials And Methods: A total of 95 patients with suspected or proven malignancy (50 patients) were admitted to the adult haematology ward at Taleghani Hospital in Tehran. Blood samples were inoculated into the bottles of Bact/Alert blood culture system and sent to Payvand's clinical and special laboratory immediately and then incubated at 35 ± 2°C.

Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis.

Background: Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit. Recently, multiplex real-time PCR has been introduced for rapid detection of the resistance genes in clinical isolates of bacteria. The current study aimed to develop and evaluate multiplex real-time PCR to detect common resistance genes among clinical isolates of A.

Antibacterial Effect of Hydroalcoholic Extract of , , and on .

Background: Considering the increased rate of microbial resistance to antibiotics and chemical side effects of antibiotics and antiseptics used for the treatment of periodontal disease, there is a need for an alternative antimicrobial agent with fewer complications. Medicinal herbs have recently become popular as novel antimicrobial agents. This study aimed to assess the antibacterial effects of hydroalcoholic extracts of , and on .

Highly Synergistic Effects of Melittin with Conventional Antibiotics Against Multidrug-Resistant Isolates of Acinetobacter baumannii and Pseudomonas aeruginosa.

Background: Morbidity and mortality due to multidrug-resistant (MDR) bacteria are of great concern in burn patients. In this critical condition, synergism between antimicrobial peptides and conventional antibiotics would be a promising strategy. Accordingly, this study aimed to determine the therapeutic value of melittin as a natural peptide by examining its synergistic effect with conventional antibiotics against MDR isolates of Acinetobacter baumannii and Pseudomonas aeruginosa.

Action mechanism of melittin-derived antimicrobial peptides, MDP1 and MDP2, de novo designed against multidrug resistant bacteria.

The emergence and dissemination of multidrug resistant (MDR) bacteria are major challenges for antimicrobial chemotherapy of bacterial infections. In this critical condition, cationic antimicrobial peptides are 'novel' promising candidate antibiotics to overcome the issue. In this study, we investigated the antibacterial mechanism of new melittin-derived peptides (i.

Role of and Genes among Imipenem Resistant Isolates from Two Hospitals of Tehran, Iran.

Background: This study aimed to evaluate the role of efflux pump regulator and genes in imipenem resistance strains isolated from hospitalized patients in Tehran, Iran.

Methods: This study was conducted on 60 isolates collected from patients admitted to the Shahid Motahari and Taleghani Hospitals in Tehran during 2013-14. Antibiotic susceptibility tests (AST) and minimal inhibitory concentration (MIC) was determined by broth micro dilution methods according to CLSI 2014 guidelines.

Evaluation of Clarithromycin Resistance Among Iranian Helicobacter pylori Isolates by E-Test and Real-Time Polymerase Chain Reaction Methods.

Background: Helicobacter pylori is an important pathogen of human gastric mucosa. Antibiotic resistance, especially resistance to clarithromycin is a major factor for treatment failure of H. pylori infections.

Efflux Pump Inhibitor Phenylalanine-Arginine Β-Naphthylamide Effect on the Minimum Inhibitory Concentration of Imipenem in Acinetobacter baumannii Strains Isolated From Hospitalized Patients in Shahid Motahari Burn Hospital, Tehran, Iran.

Background: Acinetobacter baumannii has emerged as a highly troublesome pathogen and a leading cause of mortality and morbidity among hospitalized burn patients.

Objectives: The aims of this study were to determine the frequency of the AdeABC genes and the role of the efflux pump (s) in the imipenem resistance of A. baumannii strains isolated from burn patients.

Occurrence of Potentially Pathogenic Bacterial-Endosymbionts in Acanthamoeba Spp.

Background: Acanthamoeba- bacteria interactions enable pathogenic bacteria to tolerate harsh conditions and lead to transmission to the susceptible host. The present study was aimed to address the presence of bacterial endosymbionts of Acanthamoeba isolated from recreational water sources of Tehran, Iran. To the best of our knowledge this is the first study regarding occurrence of bacteria in environmental Acanthamoeba spp.

Antibacterial and antifungal activities of the endemic species Glaucium vitellinum Boiss. and Buhse.

Objectives: Belonging to Papaveraceae family, Glaucium vitellinum is one of the Persian endemic plants which has not been investigated biologically. The present paper focused on the assessment of the antibacterial and antifungal activities of the total methanol extract and alkaloid sub-fraction of the flowering aerial parts of G. vitellinum.

Search for Proteins in the Liquid Extract of Edible Mushroom, Agaricusbisporus, and Studying their Antibacterial Effects.

The edible mushrooms (basidomycetes) have high nutritional value, promote the immune system, and as a source of natural antimicrobial substances have been used to cure bacterial infections since ancient times.Various kinds of proteins with several biological activities are produced by mushrooms. In this research, in order to evaluate antibacterial activity of edible mushrooms, we isolated proteins of Agaricus bisporus and examined their effects on gr + and gr- bacteria.

Comparing invasive and non-invasive of isolated Shigella flexneri by electron microscopy of cell culture, SDS-PAGE and Congo red method.

Background: The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods.

Methods: S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children's hospitals.