Effects of an oral adsorbent on cisplatin-induced nephropathy in rats.

A 26-week experiment was designed to determine the effect of an oral adsorbent, AST-120 (Kremezin), in rats with cisplatin-induced interstitial nephropathy. We found that creatinine clearance during the 24th week of the experiment was significantly higher in the AST-120-treated rats (n = 11) than in the control animals (n = 11) (1.09 +/- 0.

Rapid method for determining water-holding capacity in meat using video image analysis and simple formulae.

To accelerate measurement of the water-holding capacity (WHC) of meat by the press method, video image analysis (VIA) and formulae were investigated. The areas of meat and meat liquid on filter paper were little affected by changes in the operating conditions of the VIA system. VIA is associated with greater repeatability, less variation due to operators and was less time consuming than planimetry.

[Relationship between myocardial perfusion state immediately after reperfusion therapy and left ventricular wall motion improvement in patients with acute myocardial infarction].

The relationship between myocardial contrast echocardiography and improvement of the left ventricular wall motion was studied as an indicator of the prognosis of patients with acute myocardial infarction. Ten patients with acute anterior myocardial infarction who demonstrated successful reperfusion (improved to TIMI grade III) and patency of the responsible coronary artery during the chronic stage (one month after the onset) were selected. The contrast study used 2 ml of sonicated iopamidol injected into the left coronary artery.

A case of adamantinoid basal cell epithelioma.

A 56-year-old woman visited the clinic of dermatology, Yokohama Sakae Kyosai Hospital in May of 1992 with a 5-year history of a blue-gray lesion on her left cheek which had enlarged asymptomatically. The tumor was excised with Z plasty closure. Histopathologically, a loosely encapsulated tumor was situated in the upper to deep dermis.

Histological changes in bovine endometrium during the estrous cycle.

Endometrial biopsy specimens were obtained from 46 normally cyclic heifers at known stages of their estrous period to show precise characteristic changes. These tissues were embedded in paraffin, sectioned, and stained with hematoxylin and eosin. The following histological changes were observed during the estrous cycle.

Non-prostanoid thromboxane A2 receptor antagonists with a dibenzoxepin ring system. 2.

A series of 11-[2-(1-benzimidazolyl)ethylidene]-6,11-dihydrodibenz[b,e]oxep in-2- carboxylic acid derivatives and related compounds were synthesized and found to be potent TXA2/PGH2 receptor antagonists. Each compound synthesized was tested for its ability to displace [3H]U-46619 binding from guinea pig platelet TXA2/PGH2 receptors. Structure-activity relationship studies revealed that the following key elements were required for enhanced activities: (1) an (E)-2-(1-benzimidazolyl)ethylidene side chain in the 11-position of the dibenzoxepin ring system and (2) a carboxyl group in the 2-position of the dibenzoxepin ring system.

Localized multiple neurofibromas.

Two cases of localized multiple neurofibromas are reported. The patients had fairly large, closely grouped neurofibromas limited to a circumscribed part of the body, a very different clinical feature from segmental neurofibromatosis, but with otherwise had typical histopathological features. Possible aetiology is discussed.

[Electrocardiographic criteria for the diagnosis of the left septal fascicular block and its frequency among primarily elderly hospitalized patients].

Characteristic electrocardiographic findings of the left septal fascicular block consist of a prominent anterior QRS force. Therefore, the following criteria were proposed for the diagnosis of left septal fascicular block, based on the normal limits of the R and S waves and R/S of V1 and V2. (1) Right ventricular hypertrophy, complete right bundle branch block, preexcitation (Type A), high posterior infarction, hypertrophic cardiomyopathy and abnormality of the thorax or intrathoracic tissues which might cause marked counterclockwise rotation around the longitudinal axis of the heart, should be excluded.

Pathology and angiographical studies on urogenital organ system induced by idiopathic hemorrhage in male E1 mice.

Urogenital organ system induced by idiopathic hemorrhage in sudden died male E1 mice were investigated macro- and histopathologically and angiographically. The mortality of the male E1 mice with brief life span was 85% during observation period for 33 weeks, and about half of the male E1 mice died between 10-week-old and 20-week-old. In all dead mice, urine retention was prominent, and the seminal vesicle or the coagulating gland, especially the bulbocavernosus muscle had severe hemorrhage.

Latent infection of SCID mice with herpes simplex virus 1 and lethal cutaneous lesions in pregnancy.

Some SCID mice survived primary infection with herpes simplex virus 1 without the development of peripheral lesions but established coculture-positive ganglionic latency when a low dose of a wild-type strain was inoculated intracutaneously. The latency was also evidenced by the development of the fatal zosteriform skin lesions and the isolation of the virus during pregnancy. We consider that the viral entry into neurons without successive replication, rather than the arrest of the lytic infection within the cells, is an important mechanism in the establishment of latency.

Localization of herpes simplex virus type 1 in sebaceous glands of mice.

The distribution of HSV-1 during the development of zosteriform skin lesions in SCID mice was analyzed by immunofluorescence and electron microscopy. The virus initially appeared within certain keratinocytes, sometimes surrounded by keratinocytes whose surfaces were also positive for the antigens, in the lower epidermal layers including the hair follicles, and then extended upward to the entire epidermis and downward to the sebaceous glands 1-2 days later, when no macroscopic skin lesion was seen. The affected epidermal cells subsequently degenerated and lost their viral antigens within a day, when the zosteriform lesion then became evident.

Abnormal isoform of prion protein accumulates in follicular dendritic cells in mice with Creutzfeldt-Jakob disease.

We established that follicular dendritic cells (FDCs) are the site of abnormal prion protein (PrPCJD) accumulations in lymphoid tissues from mice infected with Creutzfeldt-Jakob disease. Evidence of positive FDC staining was observed in Creutzfeldt-Jakob disease-infected mice irrespective of the inoculation route, while no such staining was seen in the control mice. We also found that the severe combined immunodeficiency mouse trait is transmittable via the intracranial route but not via the intraperitoneal route.

Fine structure of atrial natriuretic peptide(ANP)-granules in the atrial cardiocytes in the pig, cattle and horse.

In the pig, cattle and horse, the right and left atria and ventricles were examined by immunohistochemistry, and the right atrial and auricular cardiocytes were studied by transmission electron microscopy. Moreover, ANP-granules in the cardiocytes were analyzed by ultrastructural morphometry. Immunohistochemically, the most intensely ANP-reacted cardiocytes were localized in the right auricle, particularly more prominent in the pig and cattle than in the horse.

An immunohistochemical study of mixed tumor of the skin.

An asymptomatic tumor developed on the upper lip of a 63-year-old man. Histologically, the tumor contained glandular and cystic structures forming many branching lumina, and many scattered single cells in an abundant mucoid to chondroid stroma. The tumor was diagnosed as mixed tumor of the skin.

Fine structure of atrial natriuretic peptide (ANP)-granules in the atrial cardiocytes of the mouse, rat and Mongolian gerbil.

Mouse, rat and Mongolian gerbil atrial and ventricular cardiocytes were examined by immunohistochemistry, and the right atrium including the auricle was examined by transmission electron microscopy. In addition the ANP granules of both right atrial and auricular cardiocytes were analyzed by ultrastructural morphometry. ANP immunoreactivity was detected in the atria of all three species, and the most intensely reacting cardiocytes were localized in the right auricular part of the atrium.

Centipede bites in Japan.

Five cases of centipede bites are reported. The patients showed erythema in response to swelling, and/or paresis around the sites. They had been bitten by Scolopendra subspinipes mutilans Koch or Scolopendra subspinipes multidens Newport.

Synthetic anthracyclines: regiospecific total synthesis of D-ring thiophene analogues of daunomycin.

The key anhydride 2-acetoxy-[2-carboxy-5-(trimethylsilyl)thiophen-3-yl]acetic acid anhydride (8), prepared from (2-carboxythiophen-3-yl)acetic acid (5), underwent a strong base-induced cycloaddition reaction with the chloroquinone acetal (11) to give the 7,7-ethylenedioxy-2-trimethylsilyl-6,7,8,9- tetrahydroanthra[2,3-b]thiophene-5,10-dione (12) regioselectively. Similarly, the regioisomeric 8,8-ethylenedioxy-2-trimethylsilyl-6,7,8,9-tetrahydroanthra[2,3-b] thiophene- 5,10-dione (30) was obtained by the strong base-induced cycloaddition reaction of 8 with the chloroquinone acetal (29). These cycloadducts (12 and 30) were converted to D-ring thiophene analogues (28 and 38) of daunomycin (1a).

Cerebral amyloid in mice with Creutzfeldt-Jakob disease is influenced by the strain of the infectious agent.

We examined Fukuoka-1 and Fukuoka-2 mouse-adapted Creutzfeldt-Jakob disease strains. Mice infected with the Fukuoka-2 strain have a higher incidence of kuru plaques, a higher concentration of proteinase-resistant prion protein, and a higher infectivity titer than do mice with the Fukuoka-1 strain. Thus, it must be kept in mind that there is a difference in the strain of the infectious agent in murine Creutzfeldt-Jakob disease.

Organ distribution of proteinase-resistant prion protein in humans and mice with Creutzfeldt-Jakob disease.

We attempted to clarify the organ distribution of human and murine proteinase-resistant prion protein (PrPCJD) in Creutzfeldt-Jakob disease (CJD), and to measure the concentration of PrPCJD, using a semi-quantitative Western blot analysis. Human PrPCJD was restricted to the central nervous system, whereas murine PrPCJD was present in the central nervous system and in the lymphoreticular system at the end stage of CJD. PrPCJD concentration in the central nervous system of mice was almost identical to that of humans.

Spotted fever group rickettsia in dogs in Japan.

Prevalence of antibody against spotted fever group-rickettsia in dogs (14/134) from the northern part of Shikoku Island, where spotted fever group rickettsia infection in human is endemic, is significantly higher than that in dogs (4/189) from nonendemic areas.

Host genetic control of incubation periods of Creutzfeldt-Jakob disease in mice.

Host genetic control of the incubation period of Creutzfeldt-Jakob disease (CJD) was studied using various inbred strains of mice, including B10 congenic strains. Various incubation periods were found in mice injected either intracerebrally or intraperitoneally with the Fukuoka 1 strain of the CJD agent; NZW/Sea and A/JJms had the shortest, and B10.AKM/Ola and C57BL/6J the longest, incubation periods.

Fine structure of the mandibular gland in volcano rabbit.

The mandibular glands of 6 male and 6 female volcano rabbits were examined by means of light and transmission electron microscopy. The acinar cells of the glands were seromucous in nature, and contained faintly basophilic granules. The cells were classified into the light cells containing granules of low or moderate densities and the clear cells having polygonal granules of low density.