The Impact of the Culture Regime on the Metabolome and Anti-Phytopathogenic Activity of Marine Fungal Co-Cultures.

Co-cultivation, coupled with the OSMAC approach, is considered an efficient method for expanding microbial chemical diversity through the activation of cryptic biosynthetic gene clusters (BGCs). As part of our project aiming to discover new fungal metabolites for crop protection, we previously reported five polyketides, the macrolides dendrodolides E () and N (), the azaphilones spiciferinone () and 8-hydroxy-spiciferinone (), and the -naphtho--pyrone cephalochromin () from the solid Potato Dextrose Agar (PDA) co-culture of two marine sediment-derived fungi, and . However, some of the purified metabolites could not be tested due to their minute quantities.