Publications by authors named "Mohammed Nasser-Ali"
Treated sewage contains a large diversity of pathogens that can be transmitted to the environment and, directly or indirectly, infect humans through water use (i.e., consumption, bathing, or irrigation).
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- * A study conducted in A Coruña, Spain, between April 2020 and February 2022 identified 252 Gram-negative bacteria with carbapenemase genes from wastewater samples, indicating a concerning level of antibiotic resistance.
- * Whole-genome sequencing of select isolates showed the potential of wastewater surveillance as an early warning system for tracking antibiotic-resistant bacteria, with notable findings preceding clinical cases at a local hospital.
The incidence of colorectal cancer (CRC) has increased worldwide, and early diagnosis is crucial to reduce mortality rates. Therefore, new noninvasive biomarkers for CRC are required. Recent studies have revealed an imbalance in the oral and gut microbiomes of patients with CRC, as well as impaired gut vascular barrier function.
View Article and Find Full Text PDFOral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28 non-CRC and 94 CRC), feces (30 non-CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM-BC package, adjusting the P-values by the Holm-Bonferroni method, revealed that Parvimonas was significantly over-represented in feces from CRC patients (P-value < 0.
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- Wastewater-based epidemiology, specifically the COVIDBENS program in A Coruña, Spain, tracked COVID-19 from June 2020 to March 2022 to provide early warnings for public health decisions.
- Using RT-qPCR and Illumina sequencing, the program monitored viral loads and detected SARS-CoV-2 mutations, significantly improving surveillance by estimating real infection rates and variant frequencies.
- The analysis identified six waves of viral load and successfully anticipated community outbreaks 8-36 days before clinical reports, enabling faster responses from local authorities and adaptation by industrial companies.
The quantification of the SARS-CoV-2 RNA load in wastewater has emerged as a useful tool to monitor COVID-19 outbreaks in the community. This approach was implemented in the metropolitan area of A Coruña (NW Spain), where wastewater from a treatment plant was analyzed to track the epidemic dynamics in a population of 369,098 inhabitants. Viral load detected in the wastewater and the epidemiological data from A Coruña health system served as main sources for statistical models developing.
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