Publications by authors named "Mohammed Hanzala Kaniyar"
J Proteins Proteom
September 2024
Article Synopsis
- Data-Independent Acquisition (DIA) enhances co-immunoprecipitation analysis by reducing quantitation variability and improving the detection of specific interactors compared to Data-Dependent Acquisition (DDA).
- A comparison of DIA and DDA across various bioinformatics workflows revealed that DIA can effectively generate spectral libraries without needing separate DDA experiments, and software struggles with indistinct signals from mock pull-downs.
- Spectronaut and DIA-NN provided the best control of coefficient of variation in protein quantification, while using DIA for both building spectral libraries and quantifying proteins leads to more consistent results and fewer missing values.
