CRISPR/Cas System: A Powerful Strategy to Improve Monogenic Human Diseases as Therapeutic Delivery; Current Applications and Challenges.

The 5,000 to 8,000 monogenic diseases are inherited disorders leading to mutations in a single gene. These diseases usually appear in childhood and sometimes lead to morbidity or premature death. Although treatments for such diseases exist, gene therapy is considered an effective and targeted method and has been used in clinics for monogenic diseases since 1989.

Machine Learning for Early Detection of Cognitive Decline in Parkinson's Disease Using Multimodal Biomarker and Clinical Data.

: Parkinson's disease (PD) is the second most common neurodegenerative disease, primarily affecting the middle-aged to elderly population. Among its nonmotor symptoms, cognitive decline (CD) is a precursor to dementia and represents a critical target for early risk assessment and diagnosis. Accurate CD prediction is crucial for timely intervention and tailored management of at-risk patients.

Aneuploidy-driven gene expression profiling in human blastocysts: insights from RNA-Seq analysis.

Purpose: Preimplantation aneuploidy in humans is one of the primary causes of implantation failure and embryo miscarriage. This study was conducted to gain insight into gene expression changes that may result from aneuploidy in blastocysts through RNA-Seq analysis.

Methods: The surplus embryos of preimplantation genetic testing for aneuploidy (PGT-A) candidate couples with normal karyotype and maternal age < 38 were collected following identical ovarian stimulation protocol.

Design and synthesis of a new recyclable nanohydrogel based on chitosan for Deltamethrin removal from aqueous solutions: Optimization and modeling by RSM-ANN.

In this study, a new magnetic biocompatible hydrogel was synthesized as an adsorbent for Deltamethrin pesticide removal. The optimal conditions and adsorption process of Deltamethrin by chitosan/polyacrylic acid/FeO nanocomposite hydrogel was studied by Response Surface Methodology by Central Composite Design (RSM-CCD) and Artificial Neural Network (ANN). This adsorbents were synthesized, and then characterized and investigated using FT-IR, XRD, FE-SEM, EDX, Map, VSM, and TGA methods.

CRISPR/Cas9 system: a novel approach to overcome chemotherapy and radiotherapy resistance in cancer.

Cancer presents a global health challenge with rising incidence and mortality. Despite treatment advances in cancer therapy, radiotherapy and chemotherapy remained the most common treatments for all types of cancers. However, resistance phenotype in cancer cells leads to unsatisfactory results in the efficiency of therapeutic strategies.

Retrograde urethrography (RUG) combined with voiding cystourethrography (VCUG) versus surgical findings in assessment of urethral strictures length.

Background And Objective: Retrograde urethrography (RUG) combined with voiding cystourethrography (VCUG) is the most common and preferred imaging modality for evaluating urethral strictures, despite its well-known limitations and disadvantages. In this study, we assessed the clinical relevance of RUG + VCUG, along with intraoperative assessment in measuring male urethral strictures.

Method: This study was a single-center retrospective study involving 134 male patients diagnosed with urethral stricture disease.

A systematic review on sustainable utilization of plastic waste in asphalt: assessing environmental and health impact, performance, and economic viability.

Increasing amount of plastic waste (PW) poses a global challenge that necessitates multifaceted strategies. Repurposing PW in asphalt pavement is a sustainable strategy with extensive benefits, but there are several challenges that need to be overcome. This systematic review aims to examine three significant aspects associated with plastic-modified asphalt: environmental and health considerations, performance and technical properties, and cost.

Assessment of the Impact of Bone Quality and Abutment Configuration on the Fatigue Performance of Dental Implant Systems Using Finite Element Analysis (FEA).

Background And Objectives: The aim of this study was to evaluate the influence of abutment angulation, types, and bone quality on fatigue performance in dental implant systems.

Materials And Methods: Three-dimensional models of maxillary 3-unit fixed implant-supported prostheses were analyzed. Abutments with different angles and types were used.

Heparin-functionalized Cu-based metal-organic framework: An efficient active and passive targeting nanocarrier for anticancer doxorubicin drug delivery.

In this study, by innovative combining the unique characteristics of Cu-based metal-organic framework (MOF) with the versatile attributes of saccharides (i.e., heparin, Hep), a promising approach is established for active and passive targeting DDS, Cu-MOF/Hep, with a pH-controlled release profile and enhanced drug efficacy.

Factors affecting the spiritual rehabilitation of people affected by natural disasters: a systematic review.

Background: Every year, natural disasters in many countries lead to the destruction of infrastructure, loss of assets, and harm to the physical, mental, social, and spiritual health of people. The attention of policymakers and the media is mostly focused on the reconstruction of damaged buildings and the physical rehabilitation and recovery of the injured, while the spiritual rehabilitation of the people affected is often neglected. This study aims to identify the factors influencing the spiritual rehabilitation of people affected by natural disasters.

An illustrated review on herbal medicine used for the treatment of female infertility.

Infertility is one of the significant global issues that affects approximately 15-17 % of couples worldwide, with around 50 % of cases being attributed to female infertility factors. The exploration of herbal extracts and their effects on female infertility has been limited, particularly regarding the underlying mechanisms beyond hormonal and oxidative stress influences. In this study, the herbal medicines with potential effects such as antioxidative properties, enhancement of insulin sensitivity, and modulation of the hypothalamic-pituitary-gonadal axis have been reviewed.

Characterisation and Application of Bio-Inspired Hybrid Composite Sensors for Detecting Barely Visible Damage under Out-of-Plane Loadings.

Traditional inspection methods often fall short in detecting defects or damage in fibre-reinforced polymer (FRP) composite structures, which can compromise their performance and safety over time. A prime example is barely visible impact damage (BVID) caused by out-of-plane loadings such as indentation and low-velocity impact that can considerably reduce the residual strength. Therefore, developing advanced visual inspection techniques is essential for early detection of defects, enabling proactive maintenance and extending the lifespan of composite structures.

Effect of macrophages combined with supernatant of mesenchymal stem cell culture and macrophage culture on wound healing in rats.

Wound healing is an orderly sequence of events restoring the integrity of the damaged tissue. It consists of inflammatory, proliferation, and remodeling phases. The objective of the current study was to investigate the effect of local transplantation of cultured macrophage loaded with mesenchymal stem cell/macrophage culture supernatants on wound healing.

Clinical and echocardiographic evaluations of sedative and cardiovascular effects of combination of xylazine-acepromazine versus xylazine-pregabalin in horses.

The aim of this study was to compare the sedative and cardiovascular effects of the combination of xylazine-acepromazine versus xylazine-pregabalin - in horses. Four healthy crossbred horses were included in the study and assigned to two treatments. In treatment I (T1), the animals received xylazine hydrochloride (1.

The global prevalence of mental health disorders among runaway and homeless youth: A meta-analysis.

A meta-analysis was performed to identify the pooled prevalence of mental health disorders (MHDs) among runaway and homeless youth (RHY). Relevant studies published between December 1, 1985, and October 1, 2023, were identified in the PubMed, Scopus, Web of Science, and Cochrane Library databases. A preliminary screening of 11,266 papers resulted in the inclusion of 101 studies.

Carboxymethyl cellulose/activated carbon/hydroxyapatite composite adsorbent for remediation of methylene blue from water media.

In the present study, activated carbon (AC), activated carbon/hydroxyapatite (AC/HAp), and carboxymethyl cellulose/activated carbon/hydroxyapatite (CMC/AC/HAp) composite adsorbents were prepared to remediation of methylene blue (MB) from water media. The pyrolysis method used the Pine cone as a natural precursor to synthesize AC. FTIR, XRD, Raman, BET, TEM, and SEM-Dot mapping techniques were applied to characterize synthesized adsorbents.

Antiviral Effect of Ferula Plants and their Potential for Treatment of COVID-19: A Comprehensive Review.

Viral diseases have always been a threat to mankind throughout history, and many people have lost their lives due to the epidemic of these diseases. In recent years, despite the progress of science, we are still witnessing a pandemic of dangerous diseases such as COVID-19 all over the world, which can be a warning for humanity. Ferula is a genus of flowering plants commonly found in Central Asia, and its species have shown antiviral activity against a variety of viruses, including respiratory syncytial virus, Herpes simplex virus type 1, influenza, human immunodeficiency virus, hepatitis B, and coronaviruses.