Sustainable bioconversion of potato peel wastes into ethanol and biogas using organosolv pretreatment.

Potato processing industries generate considerable amounts of residues, i.e., potato peel wastes (PPW).

Efficient superantioxidant and biofuel production from microalga Haematococcus pluvialis via a biorefinery approach.

A biorefinery approach was implemented to produce a superantixoident, i.e., astaxanthin, and biofuels, i.

Substrate oscillations boost recombinant protein release from Escherichia coli.

Intracellular production of recombinant proteins in prokaryotes necessitates subsequent disruption of cells for protein recovery. Since the cell disruption and subsequent purification steps largely contribute to the total production cost, scalable tools for protein release into the extracellular space is of utmost importance. Although there are several ways for enhancing protein release, changing culture conditions is rather a simple and scalable approach compared to, for example, molecular cell design.

Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut(+) strain using a two-compartment scale-down system.

Inhomogeneities in production-scale bioreactors influence microbial growth and product quality due to insufficient mixing and mass transfer. For this reason, lots of efforts are being made to investigate the effects of gradients that impose stress in large-scale reactors in laboratory scale. We have implemented a scale-down model which allows separating a homogeneous part, a stirred tank reactor (STR), and a plug flow reactor (PFR) which mimics the inhomogeneous regimes of the large-scale fermenters.

Effects of temperature shifts and oscillations on recombinant protein production expressed in Escherichia coli.

Escherichia coli is widely used host for the intracellular expression of many proteins. However, in some cases also secretion of protein from periplasm was observed. Improvement of both intracellular and extracellular production of recombinant protein in E.

Effect of post-induction substrate oscillation on recombinant alkaline phosphatase production expressed in Escherichia coli.

Microorganisms are exposed to fast changes in microenvironment in large scale bioreactors. Because of their fast response to the changes, overall performance of biological system in small scale differs from large scale. Hence the variations in the environment that microorganisms are living in are mimicked in small scale.