Optimization of the Four Most Effective Factors on β-Carotene Production by Using Response Surface Methodology.

During recent years, there was growing demand in using microalga valuable products such as β-carotene in health care. β-Carotene has anti-cancer and anti-aging properties for human. In cells, β-carotene has a major protecting role for biomolecules, when the production of reactive oxygen species is elevated.

Optimisation of the formulation of β-carotene loaded nanostructured lipid carriers prepared by solvent diffusion method.

In this study, the optimised β-carotene loaded nanostructured lipid carriers (NLC) were prepared using the solvent diffusion method. Response surface methodology (RSM) was employed in conjunction with a central composite design (CCD) to evaluate the effect of the preparation variables on particle size and β-carotene stability to optimise the NLC formulation. Quadratic polynomial was the best fitted mathematical model for the experimental results.