The amelioration of salt stress-induced damage in fenugreek through the application of cold plasma and melatonin.

Nowadays, it is increasingly crucial to combine innovative approaches with established methods to enhance plant tolerance and maximize the production of beneficial compounds. With this aim in view, a study was carried out to investigate how different melatonin concentrations (0, 30, and 60 ppm), cold plasma treatment (at 3000 and 4000 V), and varying exposure durations (0, 1, 2, and 4 min) affect the physiological and biochemical attributes of fenugreek plants, as well as the levels of diosgenin under salinity stress. This study revealed that the application of 3000 V cold plasma for 2 min with 60 ppm melatonin by establishing cellular redox homeostasis in salinity-treated fenugreek plants, effectively prevented the destruction of pigments and reduced the electrolyte leakage index of malondialdehyde content.