What is the current status of revalidation in urology?

The aim of revalidation (or maintenance of certification) is to reassure patients, the general public, employers and other healthcare professionals that an individual is fit to practice. It may lead to a reduction in near misses or adverse events. The process of revalidation entails a commitment to the provision of lifelong learning and assessment of clinical practice that ultimately ensure patient safety.

Measurements of genotoxic potential of cadmium in different tissues of fresh water climbing perch Anabas testudineus (Bloch), using the comet assay.

The present investigation was undertaken to study the induction of DNA damage by CdCl(2) in freshwater climbing perch Anabas testudineus (Bloch) using alkaline single cell gel electrophoresis (comet assay). The DNA damage was measured in the tissue of gill, kidney and liver as the percentage of DNA in comet tails and comet heads in the tissue of the fish specimens exposed to 0.1, 1.

Assessment of the level of trace element zinc in seminal plasma of males and evaluation of its role in male infertility.

Background: Male infertility is a serious problem all over the world. Nutritional deficiency of trace element Zinc (Zn) may play a role in male infertility as Zn plays an important role not only in normal testicular development, but also in spermatogenesis and sperm motility. Deficiency of Zn is associated with hypogonadism and insufficient development of secondary sex characteristics.

Analysis of surface cavity in serpin family reveals potential binding sites for chemical chaperone to reduce polymerization.

Serpin constitute about 10% of blood protein and are associated with mutations that results in aberrant intermolecular linkages which leads to polymer formation. Studies with short peptides have shown promise in depolymerization of serpins however a reactive center loop based peptide also makes the serpin inactive. A chemical chaperone based approach is a better option in terms of maintaining activity and retarding polymerization but not much is known about its binding and mechanism.

Effectiveness of procedural simulation in urology: a systematic review.

Purpose: We analyzed studies validating the effectiveness and deficiencies of simulation for training and assessment in urology. We documented simulation types (synthetic, virtual reality and animal models), participant experience level and tasks performed. The feasibility, validity, cost-effectiveness, reliability and educational impact of the simulators were also evaluated.

Insecticide-tolerant and plant-growth-promoting Rhizobium improves the growth of lentil (Lens esculentus) in insecticide-stressed soils.

Background: Application of insecticides in modern agriculture in order to enhance legume production has led to their accumulation in soils to levels that adversely affect soil microflora such as rhizobia and exert a negative impact on the physiological activities associated with them. This study was therefore designed to identify rhizobial strains expressing higher tolerance to insecticides fipronil and pyriproxyfen and synthesising plant growth regulators even amid insecticide stress.

Results: The fipronil- and pyriproxyfen-tolerant Rhizobium sp.

Assessing the genotoxic potentials of arsenic in tilapia (Oreochromis mossambicus) using alkaline comet assay and micronucleus test.

This experiment was conducted to study the genotoxic potentials of sodium arsenite (NaAsO(2)) in freshwater fish Oreochromis mossambicus by using alkaline comet assay and micronucleus (MN) test. Fish were exposed to three different concentrations (3 ppm, 28 ppm and 56 ppm) of arsenic and gill, liver and blood tissue samples were collected after 48 h, 96 h and 192 h of exposure. Arsenic exposure induced DNA damage in all tissues examined in a concentration dependent manner.

Effect of pesticides on plant growth promoting traits of greengram-symbiont, Bradyrhizobium sp. strain MRM6.

The aim of this study was to investigate the toxicity of herbicides (metribuzin and glyphosate), insecticides (imidacloprid and thiamethoxam) and fungicides (hexaconazole, metalaxyl and kitazin) at the recommended and the higher dose rates on plant growth promoting activities of Bradyrhizobium sp. under in vitro conditions. The Bradyrhizobium sp.

Risk factors for anterior cruciate ligament injury: assessment of tibial plateau anatomic variables on conventional MRI using a new combined method.

Purpose: The purpose of this study was to assess, in anterior cruciate ligament (ACL)-injured and -uninjured population, tibial plateau anatomic variables [medial and lateral tibial plateau slopes (MTPS and LTPS) and medial tibial plateau depth (MTPD)] on conventional magnetic resonance imaging (MRI) using a novel combined method and to determine whether these variables are risk factors for ACL injury.

Methods: Seventy-three isolated ACL-injury patients (20 women and 53 men) were compared with 51 control group patients (19 women and 32 men).

Results: The combined method had very high interrater and intrarater reliability compared with previously described methods.

Ecotoxicological assessment of pesticides towards the plant growth promoting activities of Lentil (Lens esculentus)-specific Rhizobium sp. strain MRL3.

This study was designed to evaluate the effect of the selected pesticides [herbicides (metribuzin and glyphosate), insecticides (imidacloprid and thiamethoxam) and fungicides (hexaconazole, metalaxyl and kitazin)] at the recommended and the higher dose rates on plant growth promoting traits of Rhizobium sp. strain MRL3 isolated from lentil-nodules. Strain MRL3 was explicitly selected owing to its high pesticide-tolerance ability and substantial production of indole acetic acid, siderophores (salicylic acid and 2, 3 dihydroxy benzoic acid), exo-polysaccharides, HCN and ammonia.

Genetic damage induced by lead chloride in different tissues of fresh water climbing perch Anabas testudineus (Bloch).

The present investigation was undertaken to study the induction of DNA damage by lead chloride (PbCl(2)) in freshwater climbing perch Anabas testudineus using alkaline single cell gel electrophoresis (comet assay). Based on the LC(50) values of lead chloride of A. testudineus three different concentrations viz.

Assessment of male reproductive health by conventional method of semen analysis.

Background: Data available over the past twenty years reveal that in approximately 30% of cases of infertility, pathology is found in man alone, and in another 20% both man and woman are abnormal. Therefore, the male factor is at least partly responsible in about 50% of infertile couples. The longer a couple remains sub fertile, the worse is their chance for an effective cure.

Repeat botulinum toxin-A injections for treatment of adult detrusor overactivity.

Intravesical injections of botulinum toxin-A have become established as an effective therapy in the management of neurogenic and idiopathic detrusor overactivity that is refractory to treatment with anticholinergic medication. The effects of the toxin are finite and repeated injections are required to sustain the beneficial therapeutic effects. The available data suggest a reproducible and sustained improvement in symptoms as well as urodynamic parameters following repeated injections of botulinum toxin-A.

Assessment of clinical utility of low and high normal alanine aminotransferase values in patients with chronic hepatitis B virus infection in Bangladesh.

Background/aims: There is a lack of consensus about the currently accepted range of normal values for serum alanine aminotransferase (ALT) levels because some investigators have claimed that the true values are significantly lower than those listed by kit manufacturers.

Methods: A total of 255 chronic hepatitis-B-virus (HBV)-infected patients with traditional, normal levels of ALT (≤42 U/l) were divided into 2 groups: (1) low normal ALT (men: ≤30 U/l; women: ≤19 U/l) and (2) high normal ALT (men: 31-42 U/l; women: 20-42 U/l). The extent of hepatic inflammation and fibrosis was evaluated in these patients by examining liver biopsy specimens.

Bacillus species enhance growth parameters of chickpea (Cicer arietinum L.) in chromium stressed soils.

Pollution of the agricultural land by the toxic chromium is a global threat that has accelerated dramatically since the beginning of industrial revolution. Toxic chromium affects both the microbial diversity as well as reduces the growth of the plants. Understanding the effect of the chromium reducing and plant growth promoting rhizobacteria on chickpea crop will be useful.

Prevalence and molecular diagnosis of Trypanosoma evansi in Nili-Ravi buffalo (Bubalus bubalis) in different districts of Punjab (Pakistan).

The prevalence of Trypanosoma evansi was investigated in 1,250 Nili-Ravi buffaloes of mixed age and sex by polymerase chain reaction (PCR) for the first time in Pakistan. DNA of the trypanosomes was isolated with TRIREAGENT®. The assay was employed using primers ESAG 6/7, specific for a 237-bp fragment from T.

Rising prevalence of Hepatitis B and C and risk factors at District Headquarter Teaching Hospital Bannu, Khyber-Pakhtunkhwa.

HBV and HCV are common health problems in developing countries like Pakistan, sharing the same mode of transmission. The purpose is to determine the prevalence of HBV and HCV, and its risk factors in Bannu. A cross sectional descriptive study was conducted in medical units of DHQTH, Bannu, from January, 2004 till December, 2008.

Clinical use of liver biopsy for the diagnosis and management of inactive and asymptomatic hepatitis B virus carriers in Bangladesh.

Patients with inactive chronic hepatitis B virus (HBV) infection are assumed to be free from liver disease. Accordingly, antiviral drug treatment is not recommended for these patients. However, the extent of liver damage in these patients has not been evaluated fully.

Assessment and maintenance of competence in urology.

Urology is becoming increasingly reliant on inter-disciplinary collaboration. As a result of this interaction and developments in technology, the existing system of training, certification and recertification needs revision. The skill-set required of urologists has become multidimensional.

A pilot study to assess the feasibility, safety and cost of robotic assisted total hysterectomy and bilateral salpingo-oophorectomy.

The aim of this study was to evaluate the safety, feasibility and cost-effectiveness of robotic assisted total hysterectomy and bilateral salpingo-oophorectomy (RATHBSO). Sixteen women underwent this new procedure for a variety of gynaecological indications. Outcome measures included operating time, estimated blood loss, length of hospital stay and cost.

Surface properties and sub-surface aggregate assimilation of rhamnolipid surfactants in different aqueous systems.

Tensioactive properties of rhamnolipids produced by a Pseudomonas aeruginosa strain were investigated in the presence or absence of Sr(2+) or Pb(2+). Surface and interfacial properties, and aggregate forming properties and morphologies were studied by various techniques including scanning electron microscopy. When the pH of a rhamnolipid aqueous solution (40 mg/l) was increased from 5 to 8, irregular vesicles gradually took the shape of oligo-vesicles, then regular vesicles and finally smaller spherical vesicles.

4-Methyl-benzyl 4-amino-benzoate.

The dihedral angle between the two benzene rings in the title compound, C(15)H(15)NO(2), is 65.28 (12)°. The crystal structure is stabilized by N-H⋯N and N-H⋯O hydrogen bonds, leading to the formation of supra-molecular chains along the a-axis direction.

Relationship between blood groups and male infertility.

Background: Blood is man's complete and unchangeable identity. The ABO and Rh groups are recognised as major and clinically significant blood groups. Blood group antigens are not only important in relation to blood transfusion and organ transplantation, but also have been utilised in genetic research, anthropology and tracing ancestral relation of humans.

Common trend of antibiotics usage in a tertiary care hospital of Peshawar, Pakistan.

Background: Antibiotics are used to eradicate the pre- and postoperative infections in surgical procedures and in all others medical cases. However, inappropriate and indiscriminate use of antimicrobial agents can potentially have a number of problems. The emergence of antimicrobial resistant bacteria, an increased number of patients experiencing adverse drug events, and increased drug-related cost have been documented.