Assessing Survival of Transgenic Bacteria, AS1 and , in Sugar Bait, White Saxaul Plant () and Rodent Barrow's Soil, A Contained-Field Study for Paratransgenesis Approach.

Background: The viability and persistence of engineered bacterium candidates in field conditions is one of the considerable challenges in the paratransgenesis approach to fighting vector-borne diseases.

Methods: In this study two engineered bacterium candidates to produce paratransgenic sand flies, AS1 and expressing m-Cherry fluorescent were applied on the leaves of the white saxaul plant (), sugar bait, and rodent burrow soil and their persistent time was tested in desert condition, Matin Abad County, Isfahan, August 2022. A PBS suspension of 10 cells/ml was used for sugar bait, spraying on plant leaves (∼10 cm) and 10 cm of rodent burrow soil.