Sensitive determination of daunorubicin in plasma of children with leukemia using pH-switchable deep eutectic solvents and HPLC-UV analysis.

An environmental friendly, fast, easy and inexpensive liquid-liquid microextraction (LLME) in combination with pH-switchable deep eutectic solvent (DES) method followed by HPLC was investigated for the separation and determination of daunorubicin (DNR) in human plasma samples. For this purpose, first, 9 DESs were prepared based on previous studies and their switchability in aqueous solution was evaluated by changing the pH. Non-switchable DESs were discarded and switchable DESs were used to extract DNR.

Sensitive determination of vincristine in plasma of children with leukaemia using vortex-assisted dispersive liquid-liquid microextraction based on hydrophobic deep eutectic solvent.

Vincristine has a wide spectrum of clinical activity and is currently used in the treatment of leukemia. Despite its high therapeutic properties, vincristine has common side effects. Accordingly, it is desirable to determine vincristine in plasma for the use of the drug with strict monitoring.

Sensitive determination of methotrexate in plasma of children with acute leukemia using double-solvent supramolecular systemas a novel extractant for dispersive liquid-liquid microextraction.

Methotrexate, as a folate antagonist, is one of the first anti-neoplasm drugs offered and is still used as an effective drug in the treatment of various malignancies. Methotrexate has a narrow treatment index and is associated with numerous side effects.In thisresearch, for the first time a double-solvent supramolecular system (DSS) was developed as an extractant without disperser solvent for dispersive liquid-liquid microextraction (DLLME).

Effects of alpha lipoic acid on iron overload, lipid profile and oxidative stress indices in β-thalassemia major patients: A cross-over randomised controlled clinical trial.

Aims: β-Thalassemia major (β-TM) is associated with iron overload, abnormal lipid levels and oxidative stress. Alpha lipoic acid (ALA) showed anti-oxidant and iron chelating properties, but its effect in β-TM patients is unclear. We investigated the effects of ALA on iron levels, lipid profile and oxidative stress.

Determination of common organophosphorus pesticides in the blood of children with acute leukaemia using a double-solvent system as a novel extractant for dispersive liquid-liquid microextraction.

In this research, a new mode of dispersive liquid-liquid microextraction based on a double-solvent system (DLLME-DSS) was developed for the extraction and preconcentration of organophosphorus pesticides (OPPs) in the blood of children with acute leukaemia prior to determination by high-performance liquid chromatography-ultraviolet detection (HPLC-UV). In the present method, two long normal chain alcohols are mixed in a particular ratio, and then injected into the sample solution, which is magnetically stirred. In this case, the mixture of the two alcohols changes to a new aggregate extractant.

Sensitive determination of deferasirox in blood of patients with thalassemia using dispersive liquid-liquid microextraction based on solidification of floating organic drop followed by HPLC-UV.

Deferasirox is an oral iron chelator that has been on the market since 2005 and has been a suitable replacement for injectable chelators. It is important to check the amount of this drug in the blood of patients due to side effects. In this study, a new dispersive liquid-liquid microextraction based on solidification of floating drganic drop (DLLME - SFO) was applied to the extraction of deferasirox in the blood of patients with thalassemia prior to its analysis by high-performance liquid chromatography-ultraviolet detection (HPLC - UV).

Effects of Alpha-Lipoic Acid Supplementation on Cardiovascular Disease Risk Factors in β-Thalassemia Major Patients: A Clinical Trial Crossover Study.

Aim: Thalassemia is one of the most common genetic diseases, and cardiovascular disease (CVD) has been considered as the leading cause of mortality in more than 50% of β-thalassemia patients. The aim of this study was to determine the effects of alpha-lipoic acid (ALA) on CVD risk factors in β-thalassemia major patients.

Methods: Twenty β-thalassemia major patients participated in this randomized crossover clinical trial study.

Optimization of a methodology for speciation of arsenic, selenium and mercury in blood samples based on the deep eutectic solvent.

In this research, a new liquid phase microextraction based on the solidification of deep eutectic solvent (LPME-SDES) has been developed for the speciation of As(III), As(V), Se(IV), Se(VI), Hg(II) and organic mercury (R-Hg) in the blood of children prior to their analysis by iridium-modified tube electrothermal atomic absorption spectrometry (ETAAS). •In this method, a green solvent consisting of Choline chloride and decanoic acid in the molar ratio of 1:2 was used as a green hydrophobic deep eutectic solvent for the extraction of complexed ions from real blood samples.•The DESs replace the toxic organic solvents apply for extraction and could be synthesize from cheap accessible chemicals.

Speciation of organic/inorganic mercury and total mercury in blood samples using vortex assisted dispersive liquid-liquid microextraction based on the freezing of deep eutectic solvent followed by GFAAS.

In this research, a new vortex assisted dispersive liquid-liquid microextraction based on the freezing of deep eutectic solvent (VADLLME-FDES) has been developed for the determination of organic mercury (R-Hg) and inorganic mercury (Hg) in blood samples prior to their analysis by graphite furnace atomic absorption spectrometry (GFAAS). In this method, a green solvent consisting of 1-octyl-3-methylimidazolium chloride and 1-undecanol was used as an extraction solvent, yielding the advantages of material stability, low density, and a suitable freezing point near room temperature. Under the optimum conditions, enrichment factor is 112.