Combining the CRISPR Activation and Interference Capabilities Using dCas9 and G-Quadruplex Structures.

We demonstrate that both CRISPR interference and CRISPR activation can be achieved at RNA and protein levels by targeting the vicinity of a putative G-quadruplex forming sequence (PQS) in the promoter with nuclease-dead Cas9 (dCas9). The achieved suppression and activation in Burkitťs Lymphoma cell line and in studies are at or beyond those reported with alternative approaches. When the template strand (contains the PQS) was targeted with CRISPR-dCas9, the G-quadruplex was destabilized and mRNA and protein levels increased by 2.

A Retrospective Analysis of Universal Health Insurance Policy-Making Process in Iran: A Qualitative Study.

Background And Aims: Universal Health Insurance (UHI) coverage began in 2014, after the implementation Health Transformation Plan (HTP) to reduce out-of-pocket payments and improve access to health services in Iran Health Insurance Organization (IHIO). The purpose of this study was to analyze the UHI policy-making process in Iran.

Methods: This study was performed using Walt and Gilson's (1994) policy triangle framework and Kingdon's multiple streams model.

Determining the influential factors of postnatal care in Bangladesh using multilevel logistic regression.

Background: Postnatal care (PNC) is the care of a newborn and mother for up to six weeks from one hour of placenta birth. The postnatal period is one of the most hazardous stages for mothers and their baby's health. The PNC is influenced by several maternal, family, biological, and socio-economic factors and it is necessary to identify the most significant factors of PNC.

Electronic referral system policy analysis: a qualitative study in the context of Iran.

Background: Referral system is among the key elements of primary health care that leads to enhanced efficiency, reduced costs, reduced waiting time, and patients' enhanced access to more specialized services. The present study was aimed at analysing the policies of the electronic referral (e-Referral) system in Iran.

Methods: This qualitative study was conducted based on Walt and Gilson's policy triangle and Kingdon's models.

The effect of changing constituents on tensile mechanical properties of HfNbTaTiZr high entropy alloy: A molecular dynamics study.

The recent trend of high-entropy alloys (HEAs) was studied extensively for their promising mechanical properties, but individual constituents' effects have remained unexplored. In this work, the effects of changing the percentage of elements of HfNbTaTiZr-HEA on the mechanical properties were analyzed during uniaxial tension using molecular dynamics simulation. The tensile strength and modulus of elastic properties of the samples were analyzed.

Reusable Microfluidic Chambers for Single-Molecule Microscopy.

Maintaining a consistent environment in single-molecule microfluidic chambers containing surface-bound molecules requires laborious cleaning and surface passivation procedures. Despite such efforts, variations in nonspecific binding and background signals commonly occur across different chambers. Being able to reuse the chambers without degrading the surface promises significant practical and fundamental advantages; however, this necessitates removing the molecules attached to the surface, such as DNA, proteins, lipids, or nanoparticles.

Ten quick tips for ensuring machine learning model validity.

Artificial Intelligence (AI) and Machine Learning (ML) models are increasingly deployed on biomedical and health data to shed insights on biological mechanism, predict disease outcomes, and support clinical decision-making. However, ensuring model validity is challenging. The 10 quick tips described here discuss useful practices on how to check AI/ML models from 2 perspectives-the user and the developer.

Exploring the relationship between blood platelet and other components utilizing count regression: A cross-sectional study in Bangladesh.

Background And Aims: Blood, vital for transporting nutrients and maintaining balance, comprises red blood cells, white blood cells, and platelets, each pivotal. Imbalances lead to issues-low red cells cause fatigue (anemia), high white cells hint at infection, low counts raise infection risks. Using trendy statistical approaches, investigating the complex link between platelet counts and numerous blood components.

Validity of machine learning algorithms for automatically extract growing rod length on radiographs in children with early-onset scoliosis.

The magnetically controlled growing rod technique is an effective surgical treatment for children who have early-onset scoliosis. The length of the instrumented growing rods is adjusted regularly to compensate for the normal growth of these patients. Manual measurement of rod length on posteroanterior spine radiographs is subjective and time-consuming.

Automated Method for Growing Rod Length Measurement on Ultrasound Images in Children With Early Onset Scoliosis.

Objective: To develop and validate machine learning algorithms to automatically extract the rod length of the magnetically controlled growing rod from ultrasound images (US) in a pilot study.

Methods: Two machine-learning (ML) models, called the "Boundary model" and "Rod model," were developed to identify specific rod segments on ultrasound images. The models were developed utilizing Mask Regional Convolutional Neural Networks (Mask RCNN).

Electronic Structure Methods for Simulating Flavin's Spectroscopy and Photophysics: Comparison of Multi-reference, TD-DFT, and Single-Reference Wave Function Methods.

The use of flavins and flavoproteins in photocatalytic, sensing, and biotechnological applications has led to a growing interest in computationally modeling the excited-state electronic structure and photophysics of flavin. However, there is limited consensus regarding which computational methods are appropriate for modeling flavin's photophysics. We compare the energies of low-lying excited states of flavin computed with time-dependent density functional theory (TD-DFT), equation-of-motion coupled cluster (EOM-EE-CCSD), scaled opposite-spin configuration interaction [SOS-CIS(D)], multiconfiguration pair-density functional theory (MC-PDFT), and several multireference perturbation theory (MR-PT2) methods.

Comprehensive molecular epidemiology of Cryptosporidium species in Japan.

Cryptosporidium species, causing diarrheal illnesses in humans and animals worldwide, are under investigation for their molecular epidemiology in Japan. The study focuses on detecting Cryptosporidium species in humans, animals, water, and the environment, revealing three species in people: C. parvum, C.

Determinants of stunting among under-five children: Evidence from Cambodian Demographic and Health Survey 2021-2022.

Background: Epidemiological and nutritional modifications are causing an increase in stunting in many low- and middle-income countries (LMIC), which will eventually result in juvenile diseases and mortality. Therefore, this study aimed to identify the influential factors contributing to stunting among under-five children in Cambodia.

Methods: A secondary dataset consisting of 3268 under-five children was extracted from the latest Cambodian Demographic and Health Survey (CDHS)-2021/2022 dataset.

Transparency interventions to improve health system outcomes in low and middle-income countries: a narrative systematic review.

Objectives: This study aimed to identify the types of transparency interventions in the health systems of the low-income and middle-income countries and the outcomes of such interventions in those systems.

Method: We searched major medical databases including PubMed, Embase and Scopus, for any kind of interventional study on transparency in health systems. We also looked for additional sources of information in organisational websites, grey literature and reference checking.

Evaluation of In Vitro Antimicrobial, Cytotoxic, Thrombolytic, and Antiarthritic Property of Different Parts of Bari Orchid.

Many different herbal extracts have historically been utilized to treat microbe-induced infections, injuries, cancer, thrombosis, and arthritis. The purpose of this study was to determine the antibacterial, cytotoxic, in vitro thrombolytic, and in vitro antiarthritic properties of ethanolic extracts of stem and seed of Bari orchid 1 (BO) plant. This orchid plant was developed by the Bangladesh Agriculture Research Institute (BARI) in Gazipur.

Economic instability, income, and unemployment effects on mortality: using SUR panel data in Iran.

This study aims to investigate the effects of socioeconomic factors on mortality in Iran. To this end, this research examines how economic instability, income, and unemployment affect mortality using a seemingly unrelated regression (SUR) with panel data for 30 provinces in Iran from 2004 to 2019. The results indicate that unemployment and mortality have a countercyclical relationship among the working age-groups 20-59 but a procyclical pattern among old-age (60+), except for rural mortality.

Stalling of Transcription by Putative G-quadruplex Sequences and CRISPR-dCas9.

Putative G-quadruplex forming sequences (PQS) have been identified in promoter sequences of prominent genes that are implicated among others in cancer and neurological disorders. We explored mechanistic aspects of CRISPR-dCas9-mediated gene expression regulation, which is transient and sequence specific unlike alternative approaches that lack such specificity or create permanent mutations, using the PQS in tyrosine hydroxylase () and promoters as model systems. We performed ensemble and single molecule investigations to study whether G-quadruplex (GQ) structures or dCas9 impede T7 RNA polymerase (RNAP) elongation process and whether orientation of these factors is significant.

Examining the molecular epidemiology of Giardia and Eimeria species in Japan: a comprehensive review.

Globally, animals and humans suffer from diarrheal illnesses due to protozoan parasites such as Giardia and Eimeria species. The molecular epidemiology of these parasites in Japan is summarized in this review. In humans, researchers found only one main species of Giardia, which is most referred to as G.

CLU (clusterin) and PPARGC1A/PGC1α coordinately control mitophagy and mitochondrial biogenesis for oral cancer cell survival.

Mitophagy involves the selective elimination of defective mitochondria during chemotherapeutic stress to maintain mitochondrial homeostasis and sustain cancer growth. Here, we showed that CLU (clusterin) is localized to mitochondria to induce mitophagy controlling mitochondrial damage in oral cancer cells. Moreover, overexpression and knockdown of CLU establish its mitophagy-specific role, where CLU acts as an adaptor protein that coordinately interacts with BAX and LC3 recruiting autophagic machinery around damaged mitochondria in response to cisplatin treatment.

MATRESHCA: Microtesla Apparatus for Transfer of Resonance Enhancement of Spin Hyperpolarization via Chemical Exchange and Addition.

We present an integrated, open-source device for parahydrogen-based hyperpolarization processes in the microtesla field regime with a cost of components of less than $7000. The device is designed to produce a batch of C and N hyperpolarized (HP) compounds via hydrogenative or non-hydrogenative parahydrogen-induced polarization methods that employ microtesla magnetic fields for efficient polarization transfer of parahydrogen-derived spin order to X-nuclei (e.g.

Prediction of phase composition and mechanical properties Fe-Cr-C-B-Ti-Cu hardfacing alloys: Modeling and experimental Validations.

Alloys of the Fe-Cr-C-B-Ti alloy system are characterized by brittleness, which can be eliminated by the copper alloy, while corrosion resistance and abrasive wear resistance are significantly reduced. In this article, comprehensive investigations are carried out on the microstructure and mechanical properties of the proposed high-copper boron-containing alloy 110Cr4Cu7Ti1VB. Systematic theoretical and experimental studies encompassed thermodynamic calculations in ThermoCALC, production of flux-cored wires for hardfacing and welding, receipt of SEM images, acquisition of load and unload diagrams (discharge) via instrumental indentation on various phases of the deposited metal, and determination of chemical composition at indentation points through local chemical analysis.

Linking green supply chain management practices and environmental performance in the manufacturing industry: a hybrid SEM-ANN approach.

This research determines the influence of green supply chain management practices (GSCM) on environmental performance. It also investigates the moderating role of supply chain environmental cooperation on GSCM practices and environmental performance relationships. A total of 370 employees of several Bangladeshi manufacturing companies were conveniently chosen as respondents.

Strategies for improving the financing of family medicine program: Evidence from Iran.

Background And Aims: The impact of health financing on the performance of the entire health system, including access, quality, and efficiency of healthcare, has been emphasized in the Astana Declaration, and the need to strengthen primary healthcare (PHC) and policy integration has been emphasized. After about two decades, the family medicine (FM) program in Iran is still facing great challenges. The aim of this study is to explore strategies for strengthening financing of the FM program in Iran, a vital component of PHC.

Graphene-like emerging 2D materials: recent progress, challenges and future outlook.

Owing to the unique physical and chemical properties of 2D materials and the great success of graphene in various applications, the scientific community has been influenced to explore a new class of graphene-like 2D materials for next-generation technological applications. Consequently, many alternative layered and non-layered 2D materials, including h-BN, TMDs, and MXenes, have been synthesized recently for applications related to the 4th industrial revolution. In this review, recent progress in state-of-the-art research on 2D materials, including their synthesis routes, characterization and application-oriented properties, has been highlighted.

Acceptability of a head-mounted assistive mouse controller for people with upper limb disability: An empirical study using the technology acceptance model.

Due to limited motor capabilities, people with upper limb disabilities have trouble utilizing a typical mouse while operating a computer. Different wearable Assistive Mouse Controllers (AMCs) have been developed to overcome their challenges. However, these people may not be able to realize the importance, ease of use, and social approval of these AMCs due to their fear of new technology, lack of confidence, and lack of ingenuity.