Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study.

Chitosan/PVA hydrogel films crosslinked by the freeze-thaw method and containing honey and allantoin were prepared for application as wound dressing materials. The effects of the freeze-thaw process and the addition of honey and allantoin on the swelling, the gel content and the mechanical properties of the samples were evaluated. The physicochemical properties of the samples, with and without the freeze-thaw process, were compared using FTIR, DSC and XRD.