Prevalence of non-extended spectrum β-lactamases SHV-1 and TEM-1 or -2 types in multidrug-resistant Enterobacteriaceae in northern Iran.

The present study aimed to evaluate TEM-1 or -2 and SHV-1 β-lactamases frequency in multidrug-resistant (MDR) Enterobacteriaceae isolated from patients' urine in northern Iran. The resistance pattern to 20 antibiotics and ESBL production in 200 MDR Enterobacteriaceae was detected using the disk diffusion test and double-disk synergy test (DDST), respectively. Multiplex PCR was applied to detect bla and bla genes in isolates.

Antibacterial Potency of Medicinal Plants including and against Multi-Drug Resistance .

Antibacterial activity of ethanolic and aqueous extracts of two medicinal plants including (EtOc, AqOc) and (EtAa, AqAa) as well as essential oil (EoAa) was investigated on multi-drug resistance (MDR) . Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) as well as the inhibition zone. The phytconstituents of these products were analyzed using Reverse-phase High- performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-mass).

Staphylococcus saprophyticus and Escherichia coli: Tracking from sperm fertility potential to assisted reproductive outcomes.

Objective: Bacteriospermia and urogenital infections are common problems in male infertility. This study aimed to evaluate the effects of bacteriospermia on sperm parameters and clinical outcomes in semen samples infected with two common bacteria (Staphylococcus saprophyticus and Escherichia coli) in northern Iran.

Methods: Microbiological tests were performed to isolate and identify organisms from 435 semen samples from infertile couples.

Design and development of a simple method for the detection and quantification of residual host cell DNA in recombinant rotavirus vaccine.

Rotavirus recombinant vaccine is usually produced in Vero cells. Residual host DNA may reside in the final product and is considered a source of contamination. WHO protocols indicate that biological products should be free of any type of impurity such as nucleic acids, endotoxins, and host cell intermediate materials.

Prevalence of plasmid-encoded carbapenemases in multi-drug resistant from patients with urinary tract infection in northern Iran.

Objectives: Resistance to carbapenems as the last line for controlling resistant bacteria is increasing due to production of carbapenemase. The aim of this study was to detect the plasmid-encoded carbapenemases using phenotypic methods and multiplex PCR among the multi-drug resistant (MDR) isolates from patients with urinary tract infection (UTI) in northern Iran.

Materials And Methods: Antimicrobial susceptibility testing and extended spectrum β-lactamase (ESBL) production test were performed for 91 MDR strains by disc diffusion and double disk synergy tests (DDST), respectively.

Investigation of antimicrobial effects of treated larvae extract on bacteria.

Background And Objectives: Larval therapy refers to the use of larvae on chronic wounds, which is a successful method of chronic wounds treatment. The secretions of these larvae contain antibacterial compounds and lead to death or inhibition of bacterial growth.

Materials And Methods: In this study, we investigated the antibacterial effects of larvae secretions which were in sterilized and multi antibiotic-resistant bacteria-treated forms on Gram-positive bacteria and Gram-negative bacteria.

Prevalence of Class 1 Integrons and Extended Spectrum Beta Lactamases among Multi-Drug Resistant Escherichia coli Isolates from North of Iran.

Background: Extended spectrum beta lactamases (ESBLs) are an important cause of transferable multidrug resistance (MDR) in gram-negative bacteria. The most described ESBL genes are generally found within integron-like structures as mobile genetic elements. The aim of this study was to identify the accompanying of class 1 integrons and ESBLs in the MDR E.