Brucella abortus antigen omp25 vaccines: Development and targeting based on Lactococcus lactis.

Background: Most Brucella infections take place on mucosal membranes. Therefore, creating vaccinations delivered through the mucosa may be crucial for managing brucellosis. Consequently, we assessed the efficacy of a recombinant oral antigen delivery system based on Lactococcus lactis for Brucella abortus omp25 antigen.

Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus.

This work aimed to provide recombinant Lactococcus lactis as a potential live vector for the manufacture of recombinant Brucella abortus (rBLS-Usp45). The sequences of the genes were collected from the GenBank database. Using Vaxijen and ccSOL, the proteins' immunogenicity and solubility were evaluated.

Trauma experiences of rural practitioners: A self assessment.

Introduction: The purpose of this study was to identify, through self-assessment, how comfortable rural emergency medicine (EM) physicians are in treating critically ill trauma patients, the resources available to treat such patients and their comfort with performing trauma procedures.

Methods: An anonymous self-assessment survey was e-mailed to family physicians practising rural EM in Saskatchewan regarding training, hospital resources, demographics and self-reported comfort with rural trauma management. We included physicians who had provided EM care within the past year in Saskatchewan outside of the major trauma centres.

Fatty acid alteration in liver, brain, muscle, and oocyte of zebrafish (Danio rerio) exposed to silver nanoparticles and mitigating influence of quercetin-supplemented diet.

No to less effort has been made to assess the toxicity of silver nanoparticles (AgNPs) to lipid composition in biological systems and also to discover a mitigating agent against their oxidative stress. Hence, this research evaluated the antioxidant capability of quercetin (Qu) against silver nanoparticles (AgNPs) toxicity towards the lipid contents of ovarian, nervous, and hepatic systems as well as skeletal muscles. To this end, zebrafish (n = 180) were assigned into four experimental dietary groups: negative and positive controls, without Qu supplementation; Qu-200, 200 mg Qu per kg diet; and Qu-400, 400 mg Qu per kg diet.

Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment.

Previous studies have shown that miR-124 plays an important role in the development of auditory neurons, which are degenerated in the sensorineural hearing loss. However, whether the combined use of miR-124 and growth factors can increase the expression of neural related markers in human dental pulp stem cells has been remained unknown so far. In this study, human dental pulp stem cells were transfected with miR-124 following treatment with brain-derived neurotrophic factor or epidermal growth factor/basic fibroblast growth factor.

Perturbation of fatty acid composition, pigments, and growth indices of Chlorella vulgaris in response to silver ions and nanoparticles: A new holistic understanding of hidden ecotoxicological aspect of pollutants.

This study assesses and compares the influence of silver nanoparticles (AgNPs) and silver nitrate (AgNO) on the fatty acid composition, pigments, and growth indices of Chlorella vulgaris. Toxicity testing was carried at the estimated and/or above predicted environmental concentrations of AgNPs and AgNO. AgNO treatments impaired the population growth of C.

Targeting the phosphoinositide 3-kinase/AKT pathways by small molecules and natural compounds as a therapeutic approach for breast cancer cells.

The phosphoinositide 3-kinase/AKT/mTOR (PI3K/AkT/mTOR) pathway plays a pivotal role in the uncontrolled growth, migration and development of human breast cancer. The elevated expression of TGF-β1 increases the PI3K/AkT/mTOR activity in human breast cancer tissue and potentially motivates tumor metastasis and resistance to chemotherapy. Here, we investigated whether treatment with PI3K/AkT/mTOR dual inhibitor NVP-BEZ235 alone or in combination with caffeic acid phenyl ester (CAPE) could prevent TGF-β1 effects on breast cancer cells.

Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877).

This study investigated the effect of individual and combination of dietary pre- and probiotics (β-glucan, 3 mg/g; mannan oligosaccharide (MOS), 4 mg/g; and Lactobacillus plantarum; 10 CFU/mg diet) on growth performance, blood immune parameters, expression of immune related genes, and intestinal microbial of Caspian trout (Salmo trutta caspius). On the basis of feeding with immunostimulant diets, the fish were assigned into eight groups denoted as: control (basal diet), bβ (basal diet + β-glucan), bM (basal diet + MOS), bLp (basal diet + L. plantarum), bβLp (basal diet + β-glucan + L.

Allopurinol and Loss of Consciousness in a 78-old Year Man Suffering from Gout.

Allopurinol is an FDA -Approved xanthine oxidase inhibitor, which is effective in the treatment of gout, hyperuricemia and uremic kidney stones in patients with an increased level of uric acid excretion. Xanthine oxidase acts by converting hypoxanthine and xanthine into uric acid, and therefore its inhibition results in decreased production of uric acid. The most common side effects of this medication are as follows: maculopapular rashes, hives, itching, headache, dizziness, abnormal hair loss, fever and hypersensitivity reaction.

Intravenous Fish Oil Lipid Emulsion Prevents Catheter-Related Thromboses in Pediatric Patients with Intestinal Failure.

A central venous catheter is a risk factor for deep vein thrombosis. We compared the incidence of deep vein thrombosis in children with intestinal failure patients receiving soy oil lipid emulsion (n = 35) vs fish oil lipid emulsion (n = 35). Ten deep vein thrombosis occurred in the soy oil lipid emulsion group, and none in the fish oil lipid emulsion group (P < .

Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway.

Background: The secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway.

Results: Using the secretion of bovine chymosin in Aspergillus awamori as a model, we found a drastic increase (40 to 80-fold) in cells grown with casein or casein phosphopeptides (CPPs). CPPs are rich in phosphoserine, but phosphoserine itself did not increase the secretion of chymosin.

Association between the length of a CA dinucleotide repeat in the EGFR and risk of breast cancer.

We studied the association of breast cancer with the polymorphic CA repeat in 108 cases of breast cancer and 108 matched controls from Isfahan city of Iran. The most common genotype in controls and patients was homozygous with allele length of 16. Our findings demonstrate that Women with two short CA repeat (< 19) are at a significantly higher risk of breast cancer, at an estimated odds ratio of 1.