Publications by authors named "Mohammad Hossein Darvishali"
Background And Purpose: M13KO7, a modified M13 phage variant, carries the p15A replication origin and Tn903 kanamycin resistance gene. This study aimed to optimize M13KO7's replication by substituting the p15A origin with the higher-copy pMB1 origin (500-700 copy numbers).
Experimental Approach: A 6431-nucleotide fragment from the M13KO7 plasmid lacking the p15A replication origin and kanamycin resistance gene was amplified using a long polymerase chain reaction (PCR).
Article Synopsis
- M13 phages are special viruses that can show parts of proteins or deliver medicine safely, which helps in making new cancer vaccines and treatments.
- They are also being used to create biosensors that can find different kinds of biological substances and human cells accurately.
- However, we still need to learn more about how M13 phages work so we can use them better in medicine, despite some challenges in getting them used more widely.