Publications by authors named "Mohammad Hasan Samiee Aref"
Article Synopsis
- * The research involved 968 participants, with genetic variants associated with body mass index (BMI) being analyzed; ultimately, five significant variants were identified.
- * Two GRS models (weighted and unweighted) showed strong predictive power for obesity, with a mean curve area of around 70%, highlighting specific genetic variants like ADRB3 rs4994 and FTO rs9939609 as having a major impact on obesity risk.
Background: The key point in the production procedure of inbred animals is checking the genetic purity. Skin grafting and coat color test are used traditionally to prove genetic purity, but they have some disadvantages. Recent advances in DNA profiling have enabled scientists to check easily the genetic purity of laboratory animals.
View Article and Find Full Text PDFArticle Synopsis
- The study focuses on HLA typing, which is crucial for matching organ transplant donors and recipients, emphasizing the need for HLA registries.
- Researchers analyzed the HLA-A locus in 100 unrelated Iranian individuals using a sequence-based method that involved PCR and Sanger sequencing.
- The findings identified A*02:01 and A*24:02 as the most common alleles, while many others were rarely found, marking it as the first report of such data for a random Iranian population to aid in donor registrations.
Objectives: To enhance the efficiency of influenza virosome-mediated gene delivery by engineering this virosome.
Results: A novel chimeric influenza virosome was constructed containing the glycoprotein of Vesicular stomatitis virus (VSV-G), along with its own hemagglutinin protein. To optimize the transfection efficiency of both chimeric and influenza cationic virosomes, HEK cells were transfected with plasmid DNA and virosomes and the transfection efficiency was assessed by FACS analysis.
Insect-derived cell lines are used extensively to produce recombinant proteins because they are capable of performing a range of post-translational modifications. Due to their significance in biotechnological applications, various methods have been developed to transfect them. In this study, we introduce a virosome constructed from vesicular stomatitis virus (VSV) as a new delivery system for sf9 cells.
View Article and Find Full Text PDF