Publications by authors named "Mohammad H Shahsavari"
Article Synopsis
- The study focuses on improving cryopreservation techniques for ovarian tissue by examining the influence of factors like corpus luteum presence and the side of the ovaries on cell viability and morphology.
- The researchers categorized bovine ovaries into six groups based on whether they had a corpus luteum and their side (right or left), finding that ovaries without a corpus luteum and from the right side showed the best tissue preservation.
- Findings indicate that recognizing the origin of the ovarian samples can enhance the success of ovarian tissue vitrification, suggesting a new approach for better outcomes in cryopreservation.
The study of the properties of engineered rocks is of great importance to researchers in engineering sciences such as petroleum, mining, and civil engineering owing to their wide application in these fields. In the present study, a physico-chemical and geomechanical investigation was carried out on the effects of different clay minerals on porous rocks. Various chemical products formed during chemical interactions between cement, clay minerals, and water can change the pore structure and thus the rock characteristics.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates how different synthetic polymers (Supercool X-1000, Supercool Z-1000, and PVP K-12) affect the vitrification process of bovine ovarian tissue, which is critical for successful preservation.
- Results showed that ovarian tissue treated with Supercool X-1000 had the highest percentage of morphologically normal follicles and stronger AQP9 labeling post-thawing than other treatments.
- However, the PVP K-12 group showed less degeneration and tissue damage, with ROS levels similar to fresh controls, suggesting that a combination of X-1000 and PVP K-12 could enhance outcomes for vitrified ovarian tissues.
Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovine ovarian tissue vitrification. From each bovine ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control group) or submitted to vitrification (sucrose, X-1000, Z-1000 and polyvinylpyrrolidone groups), either followed by culture for 1 or 5 days.
View Article and Find Full Text PDFThe aim of the current study was to investigate the effects of the presence or absence of corpus luteum (CL) on in vitro developmental competence of bovine oocytes. In experiment 1, cumulus oocyte complexes (COCs) were collected from slaughterhouse ovaries and divided according to the presence (CL(+) oocytes) or absence (CL(-) oocytes) of a CL in the ovary. Control oocytes (C group) were obtained from ovaries which were not selected toward the presence or absence of CL.
View Article and Find Full Text PDFBackground: Previous studies reported many discrepancies about the effects of corpus luteum (CL) and ovarian follicle size on the developmental competence of oocytes.
Objective: The aim of this study was to investigate the effects of CL and different size of follicle on the developmental potential of bovine oocytes.
Materials And Methods: After ovarian classification based on presence or absence of CL, sample follicles were placed in three groups according to their diameter; small (S; 3-6 mm), medium (M; 6-9 mm), and large (L; 10-20 mm).