ZnO-Rutin nanostructure as a potent antibiofilm agent against Pseudomonasaeruginosa.

Pseudomonas aeruginosa is a common human pathogen that is resistant to multiple antibiotics due to its ability to form biofilms. Developing novel nanoformulations capable of inhibiting and removing biofilms offers a promising solution for controlling biofilm-related infections. In this study, we investigated the anti-biofilm activity of rutin-conjugated ZnO nanoparticles (ZnO-Rutin NPs) in pathogenic strains of P.

Immunological characterization of recombinant outer membrane Loa22 protein of local pathogenic serovars.

Leptospirosis is a worldwide zoonotic disease caused by pathogenic spp, often occurring in tropical and subtropical regions. Focusing on development of rapid diagnostic methods to facilitate early diagnosis and a universal vaccine are the main critical issues to overcome the burden of leptospirosis. Here, we have studied the immunogenic potential of prepared recombinant Loa22 protein (rLoa22) of local pathogenic species in mice and its ability to induce humoral and cellular immunity, being further evaluated by analyzing the immunoglobulin G (IgG) subclasses and cytokines produced through immunization.

Improvement the expression and purification of Loa22: a lipoprotein with OmpA domain from pathogenic serovars.

Background And Objectives: One of the highly conserved outer membrane proteins expressed only by pathogenic Leptospires is Loa22. The study aims is to achieve the optimum conditions for high expression of recombinant Loa22 (rLoa22) protein.

Materials And Methods: Complete coding sequence of gene sub-cloned into a pET32a (+) expression vector.

Development of a newly designed biosensor using multi-walled carbon nanotubes (MWCNTs) with gold nanoparticles (AuNPs) in the presence of acetaminophen for detection of Escherichia coli.

Escherichia coli is one of the main indicators in the quality control of water, pharmaceuticals, and other samples. Compared with the time-consuming and high prices of the classical methods, with their high risks in the case of insensitive, contamination and offline detections, biosensors have long been a fast and accurate approach for identifying different bacteria. The present study reports the development of a newly electrochemical biosensor using a glassy carbon electrode (GCE) modified by multi-walled carbon nanotubes/AuNPs/E.

Antimicrobial activity of bacteriocin produced by a new Latilactobacillus curvatus sp.LAB-3H isolated from traditional yogurt.

In recent years, the use of bacteriocin-producing Lactobacillus species has received much attention in different areas, including using as probiotics, food preservation, and as broad antimicrobial spectrum activity. In this study, a bacteriocin-producing Lactobacillus strain was isolated from traditional yogurt. The isolate was identified by morphological, biochemical, 16S rRNA analyses, and designated as Latilactobacillus curvatus LAB-3H.

Optimization of zinc bioleaching from paint sludge using based on response surface methodology.

The reduction of zinc metal in the paint sludge, a hazardous waste, was investigated using by a two-stage bioleaching process. This process was performed using the response surface methodology (RSM) method based on the central composite design (CCD). Four variables, a temperature range of 32-34.

The study of stress conditions on growth and proteome of Raoultella planticola: a new emerging pathogen.

All bacteria can survive and adapt to different stresses, such as fluctuations in temperature, pH oxidative, and osmotic pressure occurring in their surrounding environments. This study aims to evaluate the effects of a variety of stress conditions on the growth, and proteome of Raoultella planticola PTCC 1598. R.

Inhibitory Potential of Acroptilon repens against Key Enzymes involved in Alzheimer and Diabetes, Phytochemical Profile, Radical Scavenging, and Antibacterial Activity.

Background: This study was devoted to assessing the inhibitory potential of acetone, methanol, and ethanol extracts of Acroptilon repens against disease-associated enzymes, as well as their antioxidant/antibacterial activity and phytochemical composition.

Methods: Comparative assessment using various antioxidant evaluation methods, including ferric reducing antioxidant power, scavenging ability on 2,2-diphenyl-1-picrylhydrazyl radical and hydrogen peroxide, and reducing power, indicated that the acetone extract presented the highest antioxidant activity, due to its highest total antioxidant content.

Results: The total phenolic content and total flavonoids content of these extracts were 3.

Rapid Detection of Escherichia coli by β-Galactosidase Biosensor Based on ZnO NPs and MWCNTs: A Comparative Study.

The need for alternative approaches for identifying pathogens has led researchers to focus on nanobiotechnology. In this study, zinc oxide nanoparticles (ZnO NPs) and multi-wall carbon nanotubes (MWCNTs) were used as marker molecules. After measuring the best concentration of these nanomaterials to inhibit the lactase activity of the beta-galactosidase enzymes by binding to them, different concentrations of Escherichia coli were added to the medium and their detection ability was finally compared with each other.

Nanoporous iron oxide nanoparticle: hydrothermal fabrication, human serum albumin interaction and potential antibacterial effects.

Nanoporous iron oxide (FeO) nanoparticles (NIONPs) have been widely used as promising agents in biomedical applications. Herein, the NIONPs were synthesized by one-step hydrothermal method and well-characterized by FESEM and TEM investigations. Afterwards, their interaction with human serum albumin (HSA) was studied using a wide range of biophysical approaches, including intrinsic and extrinsic fluorescence, far and near UV-CD, and UV-Vis spectroscopic methods as well as molecular docking investigation.

Effects of zinc chelating nutrients on Aflatoxin production in Aspergillus flavus.

Aflatoxins are carcinogenic metabolites produced by Aspergillus and Penicillium spp. Aflatoxin contamination of food is a serious health hazard. Some metal ions (such as Zn) affect Aspergillus growth and aflatoxin biosynthesis.

β-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design.

Background: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.