Publications by authors named "Mohammad B Rokni"

Article Synopsis
  • An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect specific IgG antibodies against Toxocara canis and Toxocara cati in patients with toxocariasis, analyzing serum samples.
  • The study calculated sensitivity, specificity, and predictive values for both T. canis and T. cati, showing excellent sensitivity (100% for T. canis and 97% for T. cati) and decent specificity (82% for both).
  • Five common immunoreactive bands were identified, with T. canis showing approximately twice the immune response compared to T. cati, indicating different immunological profiles in their somatic antigens.
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Article Synopsis
  • Fascioliasis is a parasitic infection caused by Fasciola species, affecting both humans and animals, yet current diagnostic methods remain under-validated despite advancements in treatment.
  • This study aimed to compare the effectiveness of Real-Time PCR with indirect-ELISA to detect Fasciola hepatica infections, using a test group consisting of patients with known infections and others with different parasitic diseases or no infections.
  • Results showed that 62.86% of the serum samples tested positive for F. hepatica, with both diagnostic methods exhibiting a high level of agreement (91.4% for ELISA and 99.2% for Real-Time PCR), confirming the reliability of these tests for diagnosing fascioliasis in humans
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Background: Identification of the larval stages of spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this study was investigate using morphological and molecular techniques.

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Background: Cystic echinococcosis (CE) is one of the most important parasitic infections in subgroup seven common neglected diseases of humans and animals. It is in the list of 18 neglected tropical diseases of the WHO. We aimed to analyze the situation of the disease in Iran using Geographical Information System (GIS) and satellite data analysis.

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Article Synopsis
  • Researchers studied over 5,300 freshwater snails from 51 habitats around the Caspian Sea in northern Iran to identify the local snail species and check for trematode infections.
  • Five families of snails were found, with Physidae being the most common; around 20% of the snails were infected with various parasites.
  • The findings suggest a significant presence of snail-borne diseases in the region, prompting calls for more research and attention from policymakers to monitor these health risks.
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Background: Calcium is a necessary mineral for life to keep the body and bones healthy. Various factors including hormones, diet, age, and gender affect serum calcium status. The aim of this sturdy was to assess the serum calcium level (SCL) of Tehran population, which has about 10 million multi-Ethnic populations and represents from the whole country.

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Article Synopsis
  • The study compared two diagnostic methods, semi-nested PCR and indirect ELISA, for detecting human fasciolosis using 70 serum samples from suspected patients in Iran.
  • Results showed that semi-nested PCR had a higher agreement rate (98.4%) compared to indirect ELISA (94.46%), with no false positives from other parasitic infections.
  • The findings suggest that semi-nested PCR is a reliable confirmatory method and can be used to monitor patient treatment effectively.
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Toxocariasis is a zoonotic parasitic disease caused by migrating nematode worms, species larvae, within tissues. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression at a post-transcriptional level. miRNA-based diagnostic biomarkers for toxocariasis are emerging, but there is limited information about the role of many miRNAs and a more detailed diagnostic evaluation of miRNA expression patterns is needed to understand their immunobiological function.

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Background: Human hydatidosis as a public concern has increased in a number of countries that have reduced control programs for the disease due to lack of resources or policies. We aimed to estimate Disability-Adjusted Life Years (DALYs) for human hydatidosis in Iran in 2018.

Methods: Data were collected from the Center of Communicable Diseases Control, Ministry of Health &Medical Education, Tehran, Iran in 2018.

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Background: Trichinellosis is a foodborne zoonosis disease worldwide. Humans acquire infection by ingesting raw or uncooked animal flesh containing viable larvae. The most common reservoirs of this helminth are pigs and wild boars.

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Article Synopsis
  • Fascioliasis is a parasitic disease found in livestock that has been identified in five confirmed cases in Lorestan Province, Iran, from January 2015 to June 2016.
  • Of 1256 patients examined, 16 tested positive for the disease, with five cases confirmed through stool tests and PCR analysis, showing a mean age of 45 and common symptoms like abdominal pain and fever.
  • The majority of patients reported consuming raw aquatic plants, particularly watercress, linking their infection to dietary habits, highlighting Lorestan as a developing area for fascioliasis with a need for further investigation.
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Background: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by . We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers.

Methods: This study was conducted on 324 individuals for ELISA test on toxocariasis in Tehran and Karaj, Iran 2019.

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Lymphatic filariasis (LF) is an important neglected parasitic disease according to the World Health Organization. In this study, we aimed to determine the prevalence of human LF in Asia using a systematic review and meta-analysis approach. Records from 1990 to 2018 in reputable databases including PubMed, Science Direct, Embase, and Cochrane Library were searched using a panel of related keywords.

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Trematodes are known as a diverse group of endogenous parasites, which snails as their intermediate hosts can dramatically affect parasite transmission dynamics. Snails play a key role in life cycles of digenean trematode. However, there has not been much faunistic surveys in terms of snails' distribution in Iran.

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Background: Cysticercosis in among the neglected tropical disease caused by eating the egg of parasite . In this review, we aimed to verify the prevalence of human cysticercosis in different countries of Asia using systematic review and meta-analysis approach.

Methods: Based of the protocol, reliable databases including PubMed, SCOPUS, Science Direct, Embase, and Cochrane Library from 1990-2018 were searched using a panel of keywords.

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Background And Objective: We conducted this study to assess the prevalence of plagiarism and to shed light on some dark aspects of this issue. The main objectives included to find out the etiology, prevalence, and detection of various forms plagiarism.

Methods: In this Cross-sectional study we used a questionnaire, face-to-face interview, analyzing the present notifications and codes, websites, and literature review.

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According to previous studies in Lorestan Province, western Iran on human fascioliasis, we aimed to understand the epidemiology of the disease and to identify the cases in rural and nomad regions of this province. The studied population was a rural and nomadic population of nine districts of Lorestan province, of which 1053 were selected according to the population of each studied county based on random sampling in 2016-2017. Initially, a questionnaire was completed for each person, including age, gender, education, occupation, use of local native aquatic plants and history of travel to the northern provinces of the country where fasciolosis has been reported mostly.

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Over the last decade, diagnostic tools to detect and differentiate species have improved, but our understanding of the distribution of haplotypes and population structure of this parasite is less clear. This study was designed to survey this gap in the epidemiology in Kermanshah province, western Iran from 2015 to 2017. Sixty-eight isolates were collected from slaughterhouses from this province.

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Background: Toxocariosis is a parasitic disease caused by the larval stage of species from dog and cat. It has a worldwide distribution with higher prevalence in children. This study aimed to determine seroprevalence of infection and its association with some risk factors among children of Aras Free Zone (Jolfa City) in Northwest of Iran.

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Background: Identification of liver flukes, , and by morphometric parameters is not always reliable due to the overlapping measurements. This study aimed to characterize the liver flukes of animals from different parts of Iran by the genetic markers, ITS1, and .

Methods: We collected flukes from infected livestock in six provinces of Iran from Sep to Nov 2016.

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Background: Cystic echinococcosis (CE), larval stage of , immunodiagnostics is still a challenge due to asymptomatic nature of CE during the early phase of infection and imperfection of diagnostic antigens. In silico design and assessments of hydatid cyst antigens provide preeminent information for novel and favorable diagnostic methods.

Methods: This study was performed at the Tehran University of Medical Sciences, Tehran, Iran in 2018.

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Background: The detection of species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related genotypes by restriction fragment length polymorphism PCR.

Methods: Seventy adult flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, south-east Iran (Jan-Jul 2017).

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Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; and . Differentiate between the two species of this genus is essential. High-Resolution Melting (HRM) Analysis represents a new approach to this issue.

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Article Synopsis
  • Fascioliasis is a disease caused by two species of parasites, but no human infections had been officially reported in Iran until now.
  • The case involved a 25-year-old woman who showed liver lesions and tested positive for the parasite through both blood and stool exams.
  • Genetic testing confirmed the parasite's identity and she was successfully treated with a single dose of Egaten®, marking this as the first reported human case of fascioliasis from this specific parasite in Iran.
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