Objective: To compare the serum nicotine levels in smokers, passive smokers and those with no exposure to cigarette smoke.
Methods: The comparative study spanning two years was conducted in Ziauddin Medical University, Karachi, in collaboration with HEJ Research Institute of Chemical Sciences, University of Karachi from 2009 to 2011 involved 135 self-reported smokers, never smokers and passive smokers among whom serum nicotine was analysed using 17-A gas chromatograph with flame ionisation detector equipped with Supelco SPB-5 fused silica capillary column (45m x 0.53mm id and 0.