Association of Two Polymorphic Codons in and Promoter with Prostate Cancer.

Background: In prostate cancer, mutated alleles typically contain missense single-base substitution in codon 72 that resides within exons 5-8. Stable proteins in tumor cell nuclei have been associated with malignancy. A role of is the regulation of drug transporters like ) by an effect on promoter region.

Isolation, cultivation and transfection of human keratinocytes.

Human keratinocytes could be used in the repair of damaged skin, in tissue engineering applications, gene therapy and recently, the generation of iPS cells. We isolated human keratinocytes from foreskin and subsequently cultured them on fibronectin, collagen type I, gelatin and laminin-coated dishes that contained three different types of serum-free medium (epilife, KSM or CnT). We developed improved conditions for efficient transfection of these human keratinocytes by testing three common transfection methods and a GFP plasmid vector.

Production of cloned mice by nuclear transfer of cumulus cells.

Background: Over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (NT) into oocytes. Although it has been 15 years since the generation of the first cloned mammals from somatic cells by NT, the success rate for producing live offspring by this technique is low regardless of the cell type and animal species used. However, these techniques have the potential to be important tools for future research in basic biology.