Quality of bovine chilled or frozen-thawed semen after addition of omega-3 Fatty acids supplementation to extender.

Background: This study was conducted to evaluate the potential protective effects of omega-3 poly unsaturated fatty acids (Ω-3 PUFAs) on bovine sperm quality in response to cooling and cryopreservation.

Materials And Methods: : In this experimental study included ejaculates from five proven fertile bulls, allocated to the control and the four experimental groups. For group 1, polyethylene glycol (PEG) as a solvent was added alone to the extender, while for groups 2, 3 and 4, different concentration of omega-3 PUFAs (1, 2.