Effect of appropriate extenders to maintain sperm functionality during short-term storage of sterlet (Acipenser ruthenus) sperm with fertilization assay under hatchery conditions.

Short-term storage and management of sperm in vitro is an easy and economical process in which suitable extenders can be utilized to extend the storage period and prevent sperm function impairment. Therefore, the current study aimed to evaluate the effect of suitable extenders during the short-term storage of sterlet sperm and determine their fertilizing capacity and hatching success. Three extenders containing a composition of 16, 20, and 24 mM NaCl, 1 mM KCl, 0.

Assessment of microplastic contamination in some commercial fishes of the southern Caspian Sea and its potential risks.

This study examined the occurrence of microplastics (MPs) in the gastrointestinal tract (GIT) of 384 fishes classified into four species from 11 sites in 2022 from the southern part of the Caspian Sea. GITs of fishes were collected and digested in HO and KOH at 45 °C for 72 h. After filtration, extracted MPs were observed under a stereomicroscope, and selected MP particles were identified using FTIR.

"Microplastic seasoning": A study on microplastic contamination of sea salts in Bangladesh.

This study investigated microplastics (MPs) in commercial sea salts from Bangladesh. The presence of MPs in the 18 sea salt bands was 100 %, where the mean MPs abundance was 471.67 MPs/kg, ranging between 300 and 670 MPs/kg.

Does the mouth size influence microplastic ingestion in fishes?

This study investigated microplastic (MP) contamination in six tropical fish species of different mouth sizes and trophic levels from Saint Martin's Island, Bay of Bengal. A total of 309 microplastics (MPs) were extracted from the gastrointestinal tract (GT) of these selected fishes, where the presence of MPs was 100 %. The mean abundance of MPs was significantly varied among the species and ranged from 4.

Microplastic contamination in commercial fish feeds: A major concern for sustainable aquaculture from a developing country.

Plastic pollution has become a global issue nowadays. Due to the increased population in developing countries, we largely depend on fish from our aquaculture industry to meet the required protein demand. Though several studies documented plastic ingestion in freshwater and marine organisms, very limited studies have been conducted to elucidate microplastic (MP) contamination in commercial fish feed.

Metal and metalloid bioaccumulation in dried red seaweed Hypnea musciformis and health risk assessment for consumers.

This study measured 22 metal and metalloid concentrations in Hypnea musciformis from the Bakkhali River estuary and Saint Martin's Island, Bangladesh and determined their potential impact on consumption. Student t-tests showed a significant variation in metal concentrations between the two sampling sites (p < 0.05).

Microplastics in the Surface Water and Gastrointestinal Tract of from the Mahodand Lake, Kalam Swat in Pakistan.

Microplastic pollution is becoming an increasingly severe environmental problem. As compared to the marine ecosystem, freshwater ecosystems at high-altitude, remote regions are less studied and lag far behind. Thus, the present study aims to highlight this issue and fill the gap in this regard.

Heavy metals and metalloids in edible seaweeds of Saint Martin's Island, Bay of Bengal, and their potential health risks.

The present study aimed to assess the levels of heavy metals and metalloids present in six seaweeds and their potential impact on consumption. The highest concentration of 11 metals, i.e.

Effect of dietary supplementation of Neem, leaf extracts on enhancing the growth performance, chemical composition and survival of rainbow trout, .

Rainbow trout has a great nutritional value and delicious taste. A 90-days experimental trial was conducted to investigate the effect of dietary leaf extract of neem tree as a feeding supplement on the growth performance and proximate composition of . Four experimental diets were designed as T (with 5% leaf extract), T (with 7% of leaf extract), T (with 10% leaf extract), and T (control group feed with a regular diet with 0% leaf extract).

Ecological risks of heavy metals on surficial sediment of Nijhum Dweep (Island), an important biodiversity area of Bangladesh.

Ten surficial sediment samples (up to a depth of 3 cm) were randomly collected during the pre-monsoon and analyzed for nine heavy metals using an atomic absorption spectrophotometer (AAS) to assess the distribution, contamination levels, and potential sources of heavy metals. The particle size distribution of the sediments was also investigated using a laser particle size analyzer. The mean concentration (mg/kg) of the analyzed metals followed a decreasing order of Fe (4706.

Microplastics in an anadromous national fish, Hilsa shad Tenualosa ilisha from the Bay of Bengal, Bangladesh.

Hilsa shad is one of the most important fisheries, contributing about 12.9% of the country's (Bangladesh) total fish production. There was no previous study on microplastic contamination of this important commercial fish.

Spatial distribution of manganese in groundwater and associated human health risk in the southern part of the Bengal Basin.

The scarcity of arsenic and iron-free safe drinking water is an alarming issue in the southern part of the Bengal Basin. The objectives of the present study were to investigate the spatial distribution of manganese (Mn) concentration in the shallow and deep groundwater and its associated health risks for the children and adults of entire southern Bengal Basin. The Mn concentration in the groundwater varied from 0 to 5.

Spatiotemporal distribution of boron in the groundwater and human health risk assessment from the coastal region of Bangladesh.

High concentrations of naturally-occurring and man-accentuated boron in groundwater possess a potential threat to the health and well-being of humans worldwide. In Bangladesh, only a few studies focused on the health risks of boron-containing groundwater. Therefore, the present study investigated the spatiotemporal distribution of boron concentrations in groundwater and its associated health risks in the coastal districts of Bangladesh.

Assessment of soil salinity in the accreted and non-accreted land and its implication on the agricultural aspects of the Noakhali coastal region, Bangladesh.

Soil salinity is a global problem that has adverse effects on both agriculture and aquaculture production. The main objectives of this study were to observe the distribution pattern of soil salinity in the accreted and non-accreted land of the Noakhali district and to determine the intensity of salinity at different depths (1-2 cm, 15-20 cm, and 45-60 cm). Soil samples from 60 sampling sites were analyzed to measure electrical conductivity (EC).

Occurrence and spatial distribution of microplastics in beach sediments of Cox's Bazar, Bangladesh.

The present study is the first attempt to investigate the occurrence and distribution pattern of microplastics (MPs) in the beach sediments covering a 36 km long beach at Cox's Bazar, Bangladesh. Fourier-transform infrared spectroscopy was used to identify the MP particles (>300 μm). The mean abundance of plastics in this study site was 8.

Optimization of sterlet (Acipenser ruthenus) egg incubation.

Sterlet Acipenser ruthenus was used to assess egg and embryo development when incubated at 17 °C in Petri dishes placed in a hatchery tank (300 L recirculating dechlorinated water) with incubation occurring in a static tabletop system in an air-conditioned laboratory, or in a 700 L Q-cell incubator. Eggs in each dish were placed in a plastic box with 300 mL dechlorinated water. Separated eggs from three individual females were fertilized using pooled sperm from four males with there being four replicates.

Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation.

The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100 μg/mL.

Proteomics: A valuable approach to elucidate spermatozoa post -testicular maturation in the endangered Acipenseridae family.

Proteomics techniques, such as two-dimensional polyacrylamide gel electrophoresis, mass spectrometry, and differential gel electrophoresis, have been extensively used to describe the protein composition of male gametes in different animals, mainly mammals. They have also provided a deeper understanding of protein functions involved in sperm processes, as in processes that in humans lead to male infertility. However, few studies focus on fish sperm proteomics and even fewer have tried to explore the proteomic profile of Sturgeon spermatozoa.

Progress and challenges of fish sperm vitrification: A mini review.

To survive low temperature is required for a long-term storage (cryopreservation), cells should be vitrified to a state in which intracellular water is solidified without ice crystal formation. Two different approaches are described for fish sperm cryopreservation: 1) sperm conventional cryopreservation, in which extracellular water is partially crystallized and 2) sperm vitrification, in which both intra- and extra-cellular liquids are vitrified. Sperm vitrification has been applied to some fish species with limited success.

Paternal identity impacts embryonic development for two species of freshwater fish.

Paternal, compared to maternal, contributions were believed to have only a limited influence on embryonic development and larval fitness traits in fishes. Therefore, the perspective of male influence on early life history traits has come under scrutiny. This study was conducted to determine parental effects on the rate of eyed embryos of Ide Leuciscus idus and Northern pike Esox lucius.

Short-term storage of sterlet Acipenser ruthenus testicular cells at -80 °C.

The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.

First report on facultative parthenogenetic activation of eggs in sterlet sturgeon, Acipenser ruthenus.

This study reported facultative parthenogenetic cleavage development of sterlet sturgeon Acipenser ruthenus eggs and quantified the percentage of parthenogenetically developed eggs in relation to the fertilization ability of different females. When eggs were activated in freshwater, 5.1-13.

Effects of preincubation of eggs and activation medium on the percentage of eyed embryos in ide (Leuciscus idus), an externally fertilizing fish.

Standardization of fertilization protocols is crucial for improving reproductive techniques for externally fertilizing fish in captive breeding. Therefore, the objectives of this study were to determine the effects of preincubation of eggs and activation medium on the percentage of eyed embryos for ide (Leuciscus idus). Pooled eggs from five females were preincubated in three different activating media for 0, 30, 60, 90, and 120 seconds and then fertilized by pooled sperm from five males.

Effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, Acipenser ruthenus.

Standardization of fertilization protocols for sterlet Acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. Our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios.