Can Prompt Endoscopic Vitrectomy in Post-Trauma Endophthalmitis with Corneal Edema Avoid Unnecessary Keratoplasties?

Objective: To describe a series of prompt endoscopic vitrectomy in eyes with post-trauma endophthalmitis and hazy cornea.

Methods: Retrospective consecutive interventional case series. Cases of endophthalmitis with hazy cornea which underwent prompt endoscopic vitrectomy were analyzed.

Endophthalmitis with opaque cornea managed with primary endoscopic vitrectomy and secondary keratoplasty: Presentations and outcomes.

Purpose: To describe the outcomes of endophthalmitis with opaque cornea managed with primary endoscopic vitrectomy and secondary keratoplasty.

Methods: Retrospective consecutive interventional case series. All cases of endophthalmitis with opaque cornea which underwent endoscopic vitrectomy followed by secondary keratoplasty were analyzed.

Dendrimer nanocarriers for transport modulation across models of the pulmonary epithelium.

The purpose of this study was to determine the effect of PEGylation on the interaction of poly(amidoamine) (PAMAM) dendrimer nanocarriers (DNCs) with in vitro and in vivo models of the pulmonary epithelium. Generation-3 PAMAM dendrimers with varying surface densities of PEG 1000 Da were synthesized and characterized. The results revealed that the apical to basolateral transport of DNCs across polarized Calu-3 monolayers increases with an increase in PEG surface density.

Quantitative detection of PLGA nanoparticle degradation in tissues following intravenous administration.

The biodegradable polymer poly(lactic-co-glycolic) acid (PLGA) has been extensively utilized and investigated as a drug delivery system. Although in vivo biodegradation (at specific administration sites only) of PLGA-based drug delivery constructs, such as foams and microparticles, has been studied, quantitative in vivo biodegradation of distributed polymer nanoparticles has not been accomplished and is quintessential for designing formulations to achieve desired pharmacokinetic properties of a drug in a target tissue. We determined the in vivo degradation kinetics of PLGA nanoparticles, of two sizes, distributed in liver, spleen, and lungs following intravenous administration.

Rapid lymph accumulation of polystyrene nanoparticles following pulmonary administration.

Purpose: Pulmonary administration of polymeric nanoparticle drug delivery systems is of great interest for both systemic and local therapies. However, little is understood about the relationship of particle size and pulmonary absorption. We investigated uptake and biodistribution of polystyrene nanoparticles (PN) of 50 nm, 100 nm, 250 nm, and 900 nm diameters in mice following administration to lungs via pharyngeal aspiration.

Quantitative nanoparticle organ disposition by gel permeation chromatography.

Gel permeation chromatography (GPC) also known as size exclusion chromatography (SEC) is a highly valuable tool for the purification, separation, and characterization of synthetic and natural polymers. In this technique, the analyte (usually a polymer) is dissolved in a suitable solvent and is injected into columns made of porous inert material. The columns separate the analyte based on its hydrodynamic size rather than the molecular weight.