miR-744/eNOS/NO axis: A novel target to halt triple negative breast cancer progression.

Background: Nitric oxide (NO) may have a dual role in cancer. At low concentrations, endogenous NO promotes tumor growth and proliferation. However, at very high concentrations, it mediates cancer cell apoptosis and inhibits cancer growth.

Targeting hydrogen sulphide signaling in breast cancer.

Introduction: Hydrogen sulphide (HS) has been established as a key member of the gasotransmitters family that recently showed a pivotal role in various pathological conditions including cancer.

Objectives: This study investigated the role of HS in breast cancer (BC) pathogenesis, on BC immune recognition capacity and the consequence of targeting HS using non-coding RNAs.

Methods: Eighty BC patients have been recruited for the study.

miRNA-506-3p Directly Regulates rs10754339 (A/G) in the Immune Checkpoint Protein B7-H4 in Breast Cancer.

Background: B7-H4 is a novel immune checkpoint protein that negatively regulates T cell activation and function. It is overexpressed in many malignant tumors, including Breast Cancer (BC). It was reported that the presence of the single nucleotide polymorphism rs10754339 (A/G) within the 3' UTR of the B7-H4 gene has a great influence on the risk and progression of BC as well as lymph node metastasis.

Contribution of and genetic variations to the incidence of acute coronary syndrome and to vitamin D serum level.

Cardiovascular diseases remain a major public health burden worldwide. It was reported that vitamin D protects the cardiovascular system through several mechanisms mainly by hindering atherosclerosis development. Genetic variations in vitamin D metabolic pathway were found to affect vitamin D levels.

Long non-coding RNAs: Functional regulatory players in breast cancer.

Historically, the long-held protein-centered bias has denoted 98% of the human genome as 'Junk' DNA. However, the current work has shifted the perception of such 'junk' transcriptional products to functional regulatory molecules. The recent surveillance of the human transcriptome has highlighted the pivotal role of such non-coding RNA (ncRNA) molecules in diverse physiological and pathological conditions.

A methoxylated quercetin glycoside harnesses HCC tumor progression in a TP53/miR-15/miR-16 dependent manner.

This study focused on studying the impact of flavonoids isolated from on HCC cell lines and to further unveil their possible impact on TP53 and its downstream tumor suppressor miRNAs. Three flavonol glycosides were isolated from namely, Isorhamnetin-3-O-β-D-glucoside Quercetin-3`-methoxy-3-O-(4``-acetylrhamnoside)-7-O-α-rhamnoside and Kaempferol-4`-methoxy-3,7-O-dirhamnoside They showed a concentration and time dependent reduction in cellular viability and anchorage-independent growth of HCC cells. Moreover, they exhibited a decrease in the migrating capacity of HepG2 cells in a pattern similar to positive control cells.

A novel role of sONE/NOS3/NO signaling cascade in mediating hydrogen sulphide bilateral effects on triple negative breast cancer progression.

Hydrogen sulphide (HS) gas has been recognized as an intracellular mediator influencing an array of signaling pathways. Yet, the role of HS in cancer progression has been controversial. This study aims to unravel the role of exogenous HS in triple negative breast cancer (TNBC) and to further investigate any possible association of HS mediated actions with the endogenous production of nitric oxide (NO) gas.

Independent assortment of GC gene polymorphism (rs2282679) and 25-hydroxyvitamin D levels in coronary artery disease.

Coronary artery disease (CAD) remains a major public health burden. Emerging research has suggested an association between vitamin D insufficiency and CAD. Vitamin D binding protein (VDBP) is the primary vitamin D carrier and many of its genetic polymorphisms are able to induce the expression of proteins with different affinities for the vitamin, which in turn might affect its serum levels and CAD incidence.

Overexpression of NMDAR2B in an inflammatory model of Alzheimer's disease: modulation by NOS inhibitors.

Background: Alzheimer's disease (AD) is a common form of age-related dementia, characterized by deposition of amyloid Aβ plaques, neuroinflammation and neurodegeneration. N-methyl-D-aspartate receptors (NMDAR) are postsynaptic glutamate receptors that play a role in memory formation and are targets for memantadine, an anti-AD drug. Nitric oxide (NO) signaling has been involved in both memory development through neuronal NO synthase (nNOS), and neuroinflammation through inducible NO synthase (iNOS) which mediates CNS inflammatory processes.

Design-of-experiment approach for HPLC analysis of 25-hydroxyvitamin D: a comparative assay with ELISA.

Although high-performance liquid chromatography-mass spectrometry (HPLC-MS) is adopted as the method of choice for the determination of vitamin D and its metabolites in plasma, yet the unavailability of this expensive detection technique in many clinical laboratories makes ultraviolet (UV) detection the alternative of choice in many places worldwide. In this regard, determination of parameters affecting HPLC separation of vitamins D2, D3 and their hydroxyl metabolites in plasma in a systematic way would put an end to irrelevant trials for more optimization. A new robust HPLC-UV was developed, optimized using DryLab(®)2000 and validated for the determination of vitamins D2 and D3 and their 25-hydroxyl metabolites in plasma to achieve best resolution and least runtime where the metabolites elute in <10 min, where vitamin D2 is considered a feasible internal standard.