Enhancement of nematicidal potential through cloning and expression of gene from subsp. BTN7A strain.

A gene encoding chitinase from has been isolated after optimization of PCR conditions. It was cloned with two different prometers, T7 promoter of the pJET1.2/blunt cloning vector and the SP6 promoter of pGEM®-T Easy vector.

Improvement of cellulose degradation by cloning of endo-β-1, 3-1, 4 glucanase () gene from BTN7A strain.

The aim of this study is to construct a new recombinant strain able to degrade cellulose efficiently. The endo-β-1, 3-1, 4 glucanase () gene was cloned from BTN7A strain by using PCR technique. The specific primers of gene were deduced.

Optimization and molecular identification of novel cellulose degrading bacteria isolated from Egyptian environment.

Cellulase producing bacteria were isolated from both soil and ward poultry, using CMC (carboxymethylcellulose) agar medium and screened by iodine method. Cellulase activity of the isolated bacteria was determined by DNS (dinitrosalicylic) acid method. The highly cellulolytic isolates (BTN7A, BTN7B, BMS4 and SA5) were identified on the basis of Gram staining, morphological cultural characteristics, and biochemical tests.