A combined therapy of Meropenem-ZnO-nanoparticles efficiently eliminates carbapenem-resistant Klebsiella pneumoniae (CRKP) biofilms, with reduced nephrotoxicity (in vitro).

In response to the World Health Organization's (WHO) research agenda of antimicrobial resistance (AMR) in human health, this study appraised the antibacterial and antibiofilm synergistic activity of meropenem and ZnO-NPs combination against carbapenem-resistant Klebsiella pneumoniae (CRKP). The minimum inhibitory concentration (MIC) of meropenem in combination was found to be approximately 1/12 of its MIC alone. The results of microtiter dilution assay showed that the combination was more efficient in reducing the biofilm biomass than meropenem alone or ZnO-NPs alone.

Standard medical ethnobotany of Kohistan, North Pakistan.

Background: This study was exclusively focused on the documentation and cross-cultural evaluation of ethnomedicinal knowledge (EMK) within the diverse linguistic groups of Kohistan situated between the Himalayan and Hindukush Mountain ranges in the north Pakistan.

Methods: Data were gathered during the field survey (May 2022 to July 2023) through group conversations, semi-structured interviews, and on-site observation. Venn diagrams were employed to illustrate the comparative assessment of EMK, and different ethnobotanical indices were utilized to examine the data.

Bioinformatics, RNA sequencing, and targeted bisulfite sequencing analyses identify the role of PROM2 as a diagnostic and prognostic biomarker.

Objectives: Prominin 2 (PROM2) gene has been reported as a molecular biomarker of human cancers; however, its role is still controversial. This study was therefore arranged to seek the role of PROM2 in different cancers with Bioinformatics and in vitro analyses.

Methods: A combination of bioinformatics and molecular experiments.

A pan-cancer analysis of pituitary tumor-transforming 3, pseudogene.

Background: Although evidence regarding pituitary tumor-transforming 3, pseudogene (PTTG3P) involvement in human cancers has been acquired via human and animal model-based molecular studies, there is a lack of pan-cancer analysis of this gene in human tumors.

Methods: Tumor-causing effects of PTTG3P in 24 human tumors were explored using The Cancer Genome Atlas (TCGA) datasets from different bioinformatics databases and applying in silico tools such as The University of ALabama at Birmingham CANcer (UALCAN), Human Protein Atlas (HPA), Kaplan Meier (KM) plotter, cBioPortal, Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), Cytoscape, Database for Annotation, Visualization, and Integrated Discovery (DAVID), Tumor IMmune Estimation Resource (TIMER), and Comparative Toxicogenomics Database (CTD). Then, via in vitro experiments, including RNA sequencing (RNA-seq) and targeted bisulfite sequencing (bisulfite-seq), expression and promoter methylation levels of PTTG3P were verified in cell lines.

Synthesis, characterization and biological investigation of zinc nanoparticles using Acacia modesta Wall. leaves.

The study aimed to synthesize zinc nanoparticles (Zn-NPs) using an aqueous extract derived from Acacia modesta Wall. leaves. Several characterization techniques were employed to confirm the successful formation of zinc nanoparticles.

Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater.

An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B.

Phytoremediation of bauxite wastewater potentiality by Jatropa curcas.

Bauxite wastewater creates soil contamination and produces toxic effects on human health such as respiratory and skin rash problems. In this study, we investigated the phytoremediation ability of Jatropha curcas to remove bauxite wastewater from soil. Pot experiments were conducted to investigate the bauxite wastewater on the phytoremediation potential of J.

Health risk assessment by toxic metals in little egrets () and food chain contaminations.

This study analysed heavy metals from little egret (). Egret's Eggs, egg shells, food (fish and insects), blood, meat samples (thigh, liver, and chest), water, soil and sediments samples were collected from the two selected sites of the study area. Samples were analysed on flame atomic absorption spectrometer after acid digestion.

Gum mediated synthesis and characterization of CuO nanoparticles towards infectious disease-causing antimicrobial resistance microbial pathogens.

Background: In this work biologically active CuO nanoparticle were discussed. The literature suggests that CuO shows very good antibacterial activity on both Gram positive and Gram-negative bacterial strains. Further, it is used in antibacterial coatings on various substrates to prevent various kinds of medical equipment's.

In-vitro antimicrobial susceptibility pattern of lipopeptide against drug resistant Vibrio species.

Background: The unrestricted application of antibiotics increased antimicrobial resistance in bacteria through horizontal gene transfer of resistant genes from the pathogenic sources and the evolution of multi-drug resistance organisms. The application of antibiotics caused severe risk to human health because animals may transmit diseases to humans. Hence, the search of novel antimicrobial agents from microbial sources is an urgent need.

Impact and fungitoxic spectrum of Trachyspermum ammi against Candida albicans, an opportunistic pathogenic fungus commonly found in human gut that causes Candidiasis infection.

Background: Trachyspermum ammi (commonly known as Ajwain), a medicinal plant of the Apiaceae family is scientifically acknowledged to harbor potential bioactive compounds for the treatment of gastrointestinal issues, loss of appetite, bronchial difficulties, cough, inflammation, diarrhoea, headache, hypertension, stomach discomfort, bronchitis and influenza. Candida albicans is generally a commensal fungus found in the gastrointestinal and genitourinary systems.

Objective: This study was focused on secondary metabolites of T.

Bioremediation of soil contaminated with toxic mixed reactive azo dyes by co-cultured cells of Enterobacter cloacae and Bacillus subtilis.

Azo dyes, known for its toxicity and mutagenicity, are used by textile industries. Bioremediation serves the best alternative treatment process due to its eco-friendly nature and cost-effectiveness. Degradation using individual bacteria promotes azo dye removal, while the degradation is enhanced using the immobilization method.

Adsorption of copper and nickel by using sawdust chitosan nanocomposite beads - A kinetic and thermodynamic study.

The adsorption behavior of biomaterial activated Sawdust-Chitosan nanocomposite beads (SDNCB) powder was investigated along with synthesis and experimental techniques approaches to study the removal efficiency of some heavy metal ions including Ni (II) and Cu (II) ions from aqueous solutions by assessing the surface-modified activated carbon by the cost-effective non-conventional method. Structural analysis of the entitled compound was evaluated by the PXRD techniques and its surface morphology was inferred by the following techniques: TEM, EDAX. The behavior of the functional group presents in the compound was discussed using the FTIR technique.

Improved photocatalytic activity for degradation of textile dyeing waste water and thiazine dyes using PbWO nanoparticles synthesized by co-precipitation method.

The coloured dyes released from the textile industrial effluents into water resources cause non-aesthetic pollution and aquatic life toxicity. Thus textile waste water treatment has been studied globally for many years. Photocatalytic properties of lead tungstate (PbWO) nanoparticles (NPs) were analyzed for thiazine dyes and textile waste water under ultraviolet light conditions.

Functionalization of gold nanoparticles by β-cyclodextrin as a probe for the detection of heavy metals in water and photocatalytic degradation of textile dye.

Gold nanoparticles (AuNPs) and AuNPs functionalized by β-cyclodextrin (β-CD/AuNPs) were prepared successfully through chemical reduction method. The structural, morphological, optical, compositional and vibrational studies for the AuNPs and β-CD/AuNPs were carried out. Functionalization of AuNPs by β-CD was confirmed with FT-IR results.

In silico prediction of silver nitrate nanoparticles and Nitrate Reductase A (NAR A) interaction in the treatment of infectious disease causing clinical strains of E. coli.

Background: The interaction of specially designed nanoparticles with proteins is the basis of formation of a nanoparticle-protein corona. Silver nanoparticles and molecules have been used in many fields due to their strong antimicrobial activity against pathogenic microorganisms such as bacteria, yeast and fungi. E.

Interactome Analysis and Docking Sites of MutS Homologs Reveal New Physiological Roles in .

Due to their sedentary lifestyle, plants are constantly exposed to different stress stimuli. Stress comes in variety of forms where factors like radiation, free radicals, "replication errors, polymerase slippage", and chemical mutagens result in genotoxic or cytotoxic damage. In order to face "the base oxidation or DNA replication stress", plants have developed many sophisticated mechanisms.