Growing conditions and seasonal fluctuations are critical factors affecting fruit and vegetable nutritional quality. The effects of two partially overlapping cropping seasons, early (ECS; January-May) and full (FCS; March-July), on the main carpometric traits and bioactive components of different watermelon fruits were investigated in the open field. Four watermelon genotypes, comprising of three commercial cultivars 'Crimson Sweet', 'Dumara', 'Giza', and the novel hybrid 'P503 F1', were compared.
View Article and Find Full Text PDFPlant growth and production are adversely affected by soil salinity. A plant growth-promoting bacteria (PGPB) designated as the "I4 strain" of Bacillus mojavensis was isolated from Tunisian soil (Sfax, Tunisia) and showed the ability to be grown in the presence of NaCl concentrations ranging from 0 to 10% in Luria Bertani (LB) medium. The PGPB-mediated salt tolerance in durum wheat was evaluated.
View Article and Find Full Text PDFPlant-specific NAC transcription factors play important roles in plant development processes, hormone signaling and response to biotic and abiotic stresses. Here, a newly identified membrane-bound NAC gene, designated as TtNTL3A, was isolated from durum wheat. TtNTL3A was homologous to bread wheat TaNAC8 and rice OsNAC8.
View Article and Find Full Text PDFUnder field conditions, wheat is subjected to single or multiple stress conditions. The elucidation of the molecular mechanism of stress response is a key step to identify candidate genes for stress resistance in plants. In this study, RNA-seq data analysis identified 17.
View Article and Find Full Text PDFThe SOD family has been extensively analyzed at genome wide level in several crops. However, little is known about this family in durum wheat. In this study, a total of 14 TdSOD genes were identified in whole durum wheat genome including 8 TdCu-ZnSODs, 2 TdMnSODs, and 4 TdFeSODs.
View Article and Find Full Text PDFBioinformatic, molecular, and biochemical analysis were performed to get more insight into the regulatory mechanism by which TmHKT1;4-A2 is regulated. HKT transporters from different plant species have been shown to play important role in plant response to salt. In previous work, TmHKT1;4-A2 gene from Triticum monococcum has been characterized as a major gene for Nax1 QTL (Tounsi et al.
View Article and Find Full Text PDFSuperoxide dismutases (SODs) play a pivotal role in improving abiotic stress tolerance in plant cells. A novel manganese superoxide dismutase gene, denoted as TmMnSOD, was identified from Triticum monococcum. The encoded protein displayed high sequence identity with MnSOD family members and was highly homologous to TdMnSOD from durum wheat.
View Article and Find Full Text PDFCatalase proteins play a crucial role in detoxifying hydrogen peroxide, generated during plant growth, and in response to various environmental stresses. Despite their importance, little is known about their localization and expression in wheat. In this study, we identified and characterized a novel peroxisomal catalase gene from Triticum monococcum, designated as TmCAT1.
View Article and Find Full Text PDFHKT transporters which mediate Na-specific transport or Na-K co-transport, play an important role in protecting plants from salinity stress by preventing Na-over-accumulation in leaves. In this work, a 1508-bp genomic fragment upstream of the TmHKT1;4-A1 translated sequence from Triticum monococcum has been isolated, cloned, and designated as the ''PrTmHKT1;4-A1'' promoter. Sequence analysis of ''PrTmHKT1;4-A1'' revealed the presence of cis-regulatory elements which could be required for abiotic stress and abscisic acid (ABA) responsiveness.
View Article and Find Full Text PDFThe ERF transcription factors belong to the AP2/ERF superfamily, one of the largest transcription factor families in plants. They play important roles in plant development processes, as well as in the response to biotic, abiotic, and hormone signaling. In the present study, 155 putative ERF transcription factor genes were identified from the potato (Solanum tuberosum) genome database, and compared with those from Arabidopsis thaliana.
View Article and Find Full Text PDFA synthetic gene encoding a single chain Fv fragment of an antibody directed against the nuclear inclusion a (NIa) protein of potato virus Y (PVY) was used to transform two commercial potato cultivars (Claustar and BF15). The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. Immunoblot analysis showed that most of the resulting transgenic plants accumulate high levels of the transgenic protein.
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