Publications by authors named "Mohamed H Aboubaker"
Article Synopsis
- Carbapenem resistance poses a significant public health risk in sub-Saharan Africa, particularly at the intersections of human, animal, and environmental factors, as shown in a study conducted in Djibouti City.
- The study analyzed 1650 samples, including urine, hospital specimens, livestock feces, fish, and water, revealing a low overall prevalence of 1.9% CP-GNB, with higher rates in fish (11.7%) and water (10%).
- Genome sequencing identified key bacterial species associated with resistance and indicated the presence of epidemic clones, suggesting horizontal gene transfer plays a role in the spread of carbapenem resistance across different environments.
Article Synopsis
- Antimicrobial resistance (AMR) is rapidly increasing globally, particularly in developing countries, with a notable rise in carbapenem-resistant bacteria, yet data from these regions is scarce.
- A study conducted in Djibouti analyzed 256 isolates, revealing that a significant majority (91.8%) were Gram-negative bacteria, with 8.5% exhibiting carbapenem resistance, predominantly from specific E. coli and other species.
- The research identified various carbapenemase genes in these resistant isolates and emphasized the importance of ongoing molecular screening to monitor and control the spread of resistance within clinical settings.
Background: The Republic of Djibouti is a malaria endemic country that was in pre-elimination phase in 2006-2012. From 2013, however, malaria has re-emerged in the country, and its prevalence has been increasing every year. Given the co-circulation of several infectious agents in the country, the assessment of malaria infection based on microscopy or histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDT) has shown its limitations.
View Article and Find Full Text PDFSince the start of COVID-19 pandemic the Republic of Djibouti, in the horn of Africa, has experienced two epidemic waves of the virus between April and August 2020 and between February and May 2021. By May 2021, COVID-19 had affected 1.18% of the Djiboutian population and caused 152 deaths.
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- - A study in Djibouti found that a significant portion of malaria cases (20.9%) were confirmed through quantitative PCR but went undetected by rapid diagnostic tests (RDTs) based on the PfHRP2 antigen.
- - Among the 79 positive samples, a whopping 86.5% were confirmed to be missing key genes targeted by these RDTs, indicating a potential issue with current diagnostic methods.
- - Given these findings, the researchers suggest the need for alternative RDTs and a comprehensive surveillance system to prevent misdiagnosis of malaria in Djibouti and similar regions where HRP2-based tests are common.
Several marine pathogens are thought to be implicated in the summer mortality phenomenon that strikes the Pacific oyster stocks (Crassostrea gigas) in Europe since more than a decade. Although, since 2008, a herpes virus variant (microvar) is considered the main responsible for juvenile mortalities, the role of several associated bacteria is less clear. One of these, Vibrio aestuarianus, has often been detected in moribund oysters, and laboratory challenges proved its involvement in oyster death.
View Article and Find Full Text PDFDuring the last decade, growing efforts have focused on human papillomavirus (HPV) detection using liquid hybridization, conventional PCR, and real-time PCR-based methods to increase the overall proportion of patients participating in cervical cancer screening procedures. We proposed a new general HPV DNA real-time PCR on the Mx4000 (Stratagene) and LightCycler (Roche Diagnostics) systems usable for both cervical scrape specimens and urine samples. A linear range was obtained from 5 DNA copies to 8 log(10) DNA copies/ml, and intra- and interassay variations were between 1.
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