Effect of melatonin, zinc sulfate, nano-melatonin, and nano-zinc oxide on mitochondria function and developmental competence of buffalo oocytes.

Objective: The aim of the current work is studying the effect of antioxidants and nano--antioxidants on development and mitochondrial function of buffalo oocytes.

Materials And Methods: Good and excellent Buffalo oocytes were matured: (1) tissue culture medium-199 (control group), (2) TCM-199 + melatonin (Mel) 10 M (Mel group), (3) TCM-199 + zinc 10 M (Zn group), (4) TCM-199 + nano- Mel 10 M (N-Mel group), and (5) TCM-199 + nano-zinc-oxide 10 M (N-ZnO group) and incubated with CO 5% and 38.5°C for 22 hr.