Optimization of Selection Agent Concentrations and Expanding G418 Utility for Gentamicin Resistance in Marchantia polymorpha.

Genetic transformation of plants is pivotal for advancing biotechnology, with success depending largely on effective selection methods. has emerged as a model plant due to its evolutionary importance, ease of manipulation, and simple genetic structure. However, inconsistent antibiotic performance and limited studies on optimal selection agent concentrations have posed challenges.

Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis.

Background: Cellular components are controlled by genetic and physiological factors that define their shape and size. However, quantitively capturing the morphological characteristics and movement of cellular organelles from micrograph images is challenging, because the analysis deals with complexities of images that frequently lead to inaccuracy in the estimation of the features. Here we show a unique quantitative method to overcome biases and inaccuracy of biological samples from confocal micrographs.

Congruence between the prioritisation of conservation problems at the local and national scale: an evaluation by environmental scientists in Poland.

The anthropogenic pressure on the environment depends on the spatial scale. It is crucial to prioritise conservation actions at different spatial scales to be cost-efficient. Using horizon scanning with the Delphi technique, we asked what the most important conservation problems are in Poland at local and national scales.