Experimental and Numerical Investigation of the Use of Ultrasonic Waves to Assist Laser Welding.

This study evaluates the enhancement of laser welding using ultrasonic waves aimed at reorganising the intermetallic position in such a fashion that leads to increased mechanical properties of welds in battery pack assemblies for electric vehicles. The experiment employed 20 kHz and 40 kHz High-Power Ultrasound Transducers (HPUTs) in both contact and contactless modes. A simplified experimental configuration is suggested to represent conditions similar to those found in electric vehicle battery pack assemblies.

Support Structures Optimisation for High-Quality Metal Additive Manufacturing with Laser Powder Bed Fusion: A Numerical Simulation Study.

This study focuses on Metal Additive Manufacturing (AM), an emerging method known for its ability to create lightweight components and intricate designs. However, Laser Powder Bed Fusion (LPBF), a prominent AM technique, faces a major challenge due to the development of high residual stress, resulting in flawed parts and printing failures. The study's goal was to assess the thermal behaviour of different support structures and optimised designs to reduce the support volume and residual stress while ensuring high-quality prints.

Analytical Simulation of the Microbubble Collapsing in a Welding Fusion Pool.

This paper explains the use of remote ultrasound vibration at the optimum position and frequencies to vibrate plates under welding, with the aim of initiating cavitation in the molten pool area. It has been shown in the literature that ultrasound cavitation changes microstructure morphology and refines the grain of the weld. In practice, the plates are excited through narrow-band high-power ultrasound transducers (HPUTs).

High-level expression of functional recombinant human coagulation factor VII in insect cells.

Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors. Mammalian cell lines provide low levels of expression, however, the Spodoptera frugiperda Sf9 cell line and baculovirus expression system are powerful systems for high-level expression of recombinant proteins, but due to the lack of endogenous vitamin K-dependent carboxylase, expression of functional FVII using this system is impossible. In the present study, we report a simple but versatile method to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cells.