This study focused on creating a specific monoclonal antibody (mAb) against the major grape allergen, Vit v1, which is a common cause of fruit allergies in Iran.
The researchers extracted the Vit v1 protein using a modified extraction method and used it to immunize mice, subsequently fusing mouse immune cells with myeloma cells to produce hybridoma cells that generate the desired antibody.
The characterization of the generated anti-Vit v1 mAb confirmed its specificity towards the Vit v1 allergen and related lipid-transfer protein allergens, suggesting its potential use in evaluating allergenicity across different grape cultivars.