High prevalence of OXA-48-like and NDM carbapenemases among carbapenem resistant of clinical origin from Iran.

Background And Objectives: is increasingly developing resistance to last-resort antibiotics such as carbapenems. This study aimed to investigate the dissemination of common carbapenemase encoding genes among 48 clinical isolates of carbapenem-resistant (CRKP).

Materials And Methods: Antimicrobial susceptibility testing was performed by broth dilution and disc diffusion methods.

Genomic features of in vitro selected mutants of Escherichia coli with decreased susceptibility to tigecycline.

Objectives: The increase in multidrug-resistant bacteria has reached an alarming rate globally, making it necessary to understand the underlying mechanisms mediating resistance in order to discover new therapeutics. Tigecycline (TGC) is a last-resort antimicrobial agent for the treatment of serious infections caused by extensively drug-resistant Enterobacteriaceae.

Methods: The TGC-resistant Escherichia coli mutants were obtained by exposing three different TGC-susceptible isolates belonging to ST131 (n = 2) and ST405 (n = 1) to increasing concentrations of TGC.

Characterization of Tigecycline Resistance Among Tigecycline Non-susceptible Isolates From Humans, Food-Producing Animals, and Selection Assay.

Emergence of extensively drug-resistant isolates of has prompted increased reliance on the last-resort antibiotics such as tigecycline (TGC) for treating infections caused by these pathogens. Consumption of human antibiotics in the food production industry has been found to contribute to the current antibiotic resistance crisis. In the current study, we aimed to investigate the mechanisms of TGC resistance among 18 TGC-non-susceptible (resistant or intermediate) (TGC-NSKP) isolates obtained from human ( = 5), food animals ( = 7), and selection experiment ( = 6).