Publications by authors named "Moeno Kadoguchi"
Article Synopsis
- Drug-induced kidney injury (DIKI) is a key factor in acute kidney injury (AKI), primarily affecting renal proximal tubular epithelial cells (RPTECs) which play a crucial role in drug processing in the kidneys.* -
- The study utilized three-dimensional cultured human RPTECs (3D-RPTECs) and found that certain drugs, like tenofovir and cisplatin, reduced ATP levels in these cells, indicating potential toxicity.* -
- 3D-RPTECs demonstrated high sensitivity (82.4% to 88.2%) and specificity (100% to 93.3%) in predicting DIKI when compared to traditional two-dimensional cell cultures, suggesting they are a valuable tool
The proximal tubule plays an important role in the kidney and is a major site of drug interaction and toxicity. Analysis of kidney toxicity via in vitro assays is challenging, because only a few assays that reflect functions of drug transporters in renal proximal tubular epithelial cells (RPTECs) are available. In this study, we aimed to develop a simple and reproducible method for culturing RPTECs by monitoring organic anion transporter 1 (OAT1) as a selection marker.
View Article and Find Full Text PDFAim: Identification of blood-brain barrier (BBB) uptake transporters is a major challenge in the research and development of central nervous system (CNS) drugs. However, conventional methods that consider known drug uptake characteristics have failed at identifying the responsible transporter molecule. The present study aimed at identifying aripiprazole uptake transporters in BBB model hCMEC/D3 cells using a knockdown screening study targeting various transporters, including uncharacterized ones.
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