Mutational dissection of a hole hopping route in a lytic polysaccharide monooxygenase (LPMO).

Oxidoreductases have evolved tyrosine/tryptophan pathways that channel highly oxidizing holes away from the active site to avoid damage. Here we dissect such a pathway in a bacterial LPMO, member of a widespread family of C-H bond activating enzymes with outstanding industrial potential. We show that a strictly conserved tryptophan is critical for radical formation and hole transference and that holes traverse the protein to reach a tyrosine-histidine pair in the protein's surface.

Complex Congenital Heart Disease and Congenital Uterine Anomalies Impacting Pregnancy Outcomes.

Complex congenital heart disease is often accompanied by extracardiac manifestations; , the significance of genitourinary involvement remains unclear. We present 3 patients with palliated complex congenital heart disease and with pregnancies complicated by premature delivery who were found to have congenital uterine anomalies that may have contributed to their obstetrical complications.

The speed-gene study: methods, study design and preliminary results.

The Speed-Gene study aims to identify genetic variants influencing athletic performance and human locomotion using motion capture technology. Currently, 60 female participants have completed the testing protocol, and the overall aim is to recruit 283 moderately trained, healthy Southeast Asian individuals (18-45 y, BMI < 30). Participants will undergo biomechanical analysis and genetic testing.

Development of Epstein-Barr virus-associated gastric cancer: Infection, inflammation, and oncogenesis.

Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) cells originate from a single-cell clone infected with EBV. However, more than 95% of patients with gastric cancer have a history of () infection, and is a major causative agent of gastric cancer. Therefore, it has long been argued that infection may affect the development of EBVaGC, a subtype of gastric cancer.

Effective treatment of diabetes, improved quality of life and accelerated cognitive development after pancreas transplantation in a child with type 1 diabetes and allergy to manufactured insulin preparations.

Background: Insulin hypersensitivity reactions are rare but serious and significantly affect the treatment of diabetes in children.

Methods: A 13-year-old girl with type 1 diabetes, hypoglycemic unawareness, and treatment refractory allergy to available insulin preparations underwent a solitary pancreas transplant. Before the pancreas transplantation, she was receiving a continuous subcutaneous infusion of rapid-acting insulin with an increasing need for antihistamines and steroids, negatively impacting her cognitive and social development.

Characterization of Endophytic Fungi, sp., from and Analysis of Its Antifungal and Plant Growth-Promoting Effects.

The present study was aimed at isolating endophytic fungi from the Asian culinary and medicinal plant and analyzing its antifungal and plant growth-promoting effects. In this study, the fungal endophyte sp. Ld-03 was isolated from the bulbs of and identified through morphological and molecular analysis.

Music therapy was noninferior to verbal standard treatment of traumatized refugees in mental health care: Results from a randomized clinical trial.

: Many people with refugee backgrounds suffer from trauma-related complex social and psychological problems, and compliance with standard psychological treatment tends to be low. More culturally adaptable treatment options seem to be needed. : We aimed to investigate whether the music therapy method: 'trauma-focused music and imagery' (tr-MI), characterized by a particular focus on arousal and affect regulation, would be equally effective as the standard psychological talk therapies for ameliorating trauma symptoms in Danish refugees.

[A man in his forties with rapid exacerbation of chronic foot ulcers].

Background: The development of skin cancer, especially squamous cell carcinoma, is a well-known but rare and often under-recognised complication of chronic wounds and longstanding scar tissue. These skin cancers, known as Marjolin's ulcer, are more aggressive in nature than similar cutaneous cancers of different etiology, with a higher rate of local recurrence and an increased metastatic potential.

Case Presentation: Our patient suffered severe trauma to his left leg and foot in a car accident during childhood and underwent extensive reconstruction.

Unicommissural Unicuspid Aortic Valve, Ascending Aortic Aneurysm, and Bovine Arch: A Rare Case Report.

A 24-year-old man presented with rapidly progressive dyspnea due to mixed aortic stenosis and insufficiency. Unicommissural unicuspid aortic valve, ascending aortic aneurysm, and a bovine arch were identified on computed tomography angiography. Uncomplicated surgical mechanical valve replacement and ascending aortic graft placement improved his symptoms.

Tissue Nutrient Concentrations and Stoichiometry in Oligotrophic Ecosystems: Variability with Seasons, Growth Forms, Organs and Habitats.

Aquatic plant nutrient concentrations provide important information to characterise their role in nutrient retention and turnover in aquatic ecosystems. While large standing biomass of aquatic plants is typically found in nutrient-rich localities, it may also occur in oligotrophic ecosystems. is able to form massive stands even in very nutrient-dilute waters.

Boesenmaxane Diterpenoids from .

Three new diterpenoids, boesenmaxanes A-C (-), with an unprecedented core skeleton consisting of an unusual C-C bond between C-12 and an -cyclic methylene C-13, were isolated from the rhizome extracts of . The structures were elucidated by analysis of spectroscopic and X-ray diffraction data. Electronic circular dichroism spectra were used to determine the absolute configuration.

The complete chloroplast genome sequence of Var. , an important breeding parent.

var. is an important breeding parent with high ornamental and edible value in worldwide. In this study, we reported a complete chloroplast genome of var.

Wnt Signaling Through Nitric Oxide Synthase Promotes the Formation of Multi-Innervated Spines.

Structural plasticity of synapses correlates with changes in synaptic strength. Dynamic modifications in dendritic spine number and size are crucial for long-term potentiation (LTP), the cellular correlate of learning and memory. Recent studies have suggested the generation of multi-innervated spines (MIS), in the form of several excitatory presynaptic inputs onto one spine, are crucial for hippocampal memory storage.

Hepatocellular carcinoma and the Fontan circulation: Clinical presentation and outcomes.

Background: Fontan-associated liver disease (FALD) is universal in patients with a Fontan circulation. Hepatocellular carcinoma (HCC) is one of its severe expressions, and, though rare, frequently fatal. The purpose of this study was to describe the clinical presentation, risk factors, and outcomes of HCC in patients with a Fontan circulation.

Acute Coronary Syndrome and Ischemic Heart Disease in Pregnancy: Data From the EURObservational Research Programme-European Society of Cardiology Registry of Pregnancy and Cardiac Disease.

Background The prevalence of ischemic heart disease (IHD) in women of child-bearing age is rising. Data on pregnancies however are scarce. The objective is to describe the pregnancy outcomes in these women.