Biodiversity of Aflatoxigenic Species in Dairy Feeds in Bulawayo, Zimbabwe.

The presence of molds, especially certain species of , in food commodities may contribute to aflatoxin contamination. The aim of this study was to determine the biodiversity of species in dairy feeds from farms in select locations in Zimbabwe and assess their aflatoxin production potential using a polyphasic approach. A total of 96 feed samples were collected, which consisted of dairy feed concentrate, mixed ration, brewers' spent grain, and grass from 13 farms during the dry season (August-October, 2016) and the following rainy season (January-March, 2017).

Phylogenetic diversity and prevalence of mycoflora in ready-to-eat supermarket and roadside-vended peanuts.

Little is known of the mycobiota present in ready-to-eat peanuts consumed in Southern Africa. Knowledge of the mycobiota and aflatoxigenic species can elucidate potential health risks associated with consumption of ready-to-eat peanuts sold by supermarkets and roadside vendors. We investigated the culturable mycobiota diversity in supermarket and roadside-vended peanuts as well as the presence of five aflatoxin biosynthesis pathway-related genes (, and ) in 15 suspected aflatoxigenic isolates, with a focus on Mafikeng, South Africa.

Fungal metabolite and mycotoxins profile of cashew nut from selected locations in two African countries.

Infestation of food crops by fungi has led to serious economic losses in the international market. There is a paucity of information on the multi-mycotoxin profile of cashew nuts from different geographical locations in Nigeria and South Africa. This work aims at comparing the fungal metabolite profile of cashew nut from two African countries using HPLC-FLD and LC-MS/MS.

A polyphasic method for the identification of aflatoxigenic Aspergilla from cashew nuts.

The invasion of food by toxigenic fungi is a threat to public health. This study aimed at enumerating the microbial profile, detection of aflatoxin producing genes and quantification of the levels of aflatoxin contamination of cashew nuts meant for human consumption. A polyphasic method of analysis using newly formulated β-Cyclodextrin Neutral Red Desiccated coconut agar (β-CDNRDCA) and Yeast Extract Sucrose agar (YES) with Thin Layer Chromatography (TLC), Polymerase Chain Reaction (PCR) and High Performance Liquid Chromatographic (HPLC) method was adopted in determining the aflatoxigenic potential of the isolates, the presence of aflatoxin biosynthetic gene (aflM, aflD, aflR, aflJ omt-A) and estimation of the total aflatoxin content of the nuts.

Molecular Characterization and Antibiotic Resistance of Foodborne Pathogens in Street-Vended Ready-to-Eat Meat Sold in South Africa.

Abstract: The consumption of food contaminated with microbial populations remains a key route of foodborne infection in developing countries and creates a serious public health burden. This study aimed at identifying foodborne pathogens and their antibiotic resistance profiles in ready-to-eat meat sold in public eateries in the Johannesburg area. A total of 115 samples were examined for the incidence of bacteria pathogens and their antibiotic resistance profiles against commonly used antibiotics (ampicillin, tetracycline, chloramphenicol, erythromycin, ciprofloxacin, streptomycin, and sulphonamides) using the molecular and the disc diffusion methods.

Microbiological Quality and Risk Assessment for Aflatoxins in Groundnuts and Roasted Cashew Nuts Meant for Human Consumption.

Nuts are one of the commonly consumed snacks but poor handling and storage practices can make them prone to foodborne infections. The study aimed at assessing the microbiological quality and risk assessment for aflatoxins in groundnuts and cashew nuts consumed in selected locations in Nigeria. The moisture content, colony counts, incidence of pathogenic bacteria, aflatoxin contamination, and risk assessment for aflatoxins were evaluated using standard methods.

Current Status of Mycotoxin Contamination of Food Commodities in Zimbabwe.

Agricultural products, especially cereal grains, serve as staple foods in sub-Saharan Africa. However, climatic conditions in this region can lead to contamination of these commodities by moulds, with subsequent production of mycotoxins posing health risks to both humans and animals. There is limited documentation on the occurrence of mycotoxins in sub-Saharan African countries, leading to the exposure of their populations to a wide variety of mycotoxins through consumption of contaminated foods.

Evaluation of Antibiotic Residues in Raw Meat Using Different Analytical Methods.

Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat from sales points were analysed for ciprofloxacin, streptomycin, tetracycline, and sulphanilamide residues.

Risk Assessment of Mycotoxins in Stored Maize Grains Consumed by Infants and Young Children in Nigeria.

Maize is a major complimentary food for infants (0-4years) and young children (5-12years) in Nigeria. In this study, we assessed the risk of exposure of infants and young children (IYC) to some major mycotoxins in stored maize grains from five agro-ecological zones of Nigeria. The probable daily intake approach was employed to determine exposure to five mycotoxins while the margin of exposure (MOE) and population at risk of primary hepatocellular carcinoma approaches were used to characterize the risk of consuming aflatoxin contaminated maize.

Mycotoxin risk assessment for consumers of groundnut in domestic markets in Nigeria.

The fungal and multi-mycotoxin profiles of groundnuts sold in domestic markets in Nigeria as well as the associated risk to consumers were assessed in the present study. Four hundred fungal isolates representing mainly Aspergillus [58.6%: Aspergillus section Flavi (37.

Fungal and bacterial metabolites of stored maize (Zea mays, L.) from five agro-ecological zones of Nigeria.

Seventy composite samples of maize grains stored in five agro-ecological zones (AEZs) of Nigeria where maize is predominantly produced were evaluated for the presence of microbial metabolites with the LC-MS/MS technique. The possible relationships between the storage structures and levels of mycotoxin contamination were also evaluated. Sixty-two fungal and four bacterial metabolites were extracted from the grains, 54 of which have not been documented for maize in Nigeria.