Development of single mouse blastomeres enlarged to zygote size in conditions of nucleo-cytoplasmic synchrony.

The following blastomeres were enlarged to the size of the zygote by one, two or three rounds of blastomere enucleation and electrofusion: (1) from the 2-cell stage (referred to as 2/1 embryos), (2) from the 4-cell stage (referred to as 4/1 embryos), (3) from the 8-cell stage (referred to as 8/1 embryos). Such single enlarged blastomeres developed into blastocysts in vivo in 55.5% (2/1), 28% (4/1) and 6.

Immune monitoring after renal transplantation. II. Post-transplant disturbances in the CD3 complex expression may be caused by immunosuppression.

The in vitro effect of major immunosuppressive agents on the CD3-TCR complex expression on T cells was studied. Cyclosporine and azathioprine caused a marked diminution of alpha/beta, but not gamma/delta and CD3 chains detectability. Prednisolone effect was less manifested.

Immune monitoring after renal transplantation. I. HLA-D expression on T cells from renal allograft recipients.

The expression of class II antigens (HLA-DR, DP and DQ) on resting and PHA-activated T cells from renal allograft recipients was studied. A tendency for increase in DR+/DQ+ T cells was noted in azathioprine and cyclosporine-treated recipients undergoing rejection. Low inducible HLA-D expression (especially DP) was associated with CMV infection.

Normal B cell compartment in chronic lymphatic leukemia.

In contrast to normal B lymphocytes, CLL cells express CD5 antigen believed to be a T cell marker. We have assessed normal and malignant B cell compartments in CLL patients with the aid of two-colour immunofluorescence techniques (CD5-TRITC, DR-FITC). No clear correlation was found between progression of disease and parameters studied.

Rejection-associated upregulation of the alpha/beta-T cell receptor expression on T cells of renal allograft recipients.

Peripheral T lymphocytes of renal allograft recipients were phenotyped for their expression of the CD3-T cell receptor (TCR) complex. The majority of T cells from patients with stable graft function had normal CD3 but diminished TCR alpha/beta heterodimer expression, while TCR gamma/delta + T cells were increased in some cases. Acute rejection was associated with an increase in TCR alpha/beta + T cells to normal levels.

Immunosuppressive drugs inhibit class II antigen (HLA-D) synthesis by B and T lymphocytes.

The influence of immunosuppressive drugs on class II (HLA-D) antigen synthesis and expression by monocytes and B and T cells was studied. The constitutive HLA-D expression on monocytes and malignant B cells was resistant, while the acquisition of those markers by stimulated T cells very sensitive to immunosuppression. Active derivative of cyclophosphamide (4HCy) and cyclosporine proved to be most efficacious inhibitors of class II antigens.

Large granular lymphocyte lymphocytosis with neutropenia: a case report.

A case of large granular lymphocyte lymphocytosis with neutropenia was studied as evidenced by peripheral lymphocytosis of cells having typical morphology and profound neutropenia. Surface markers analysis revealed that almost all cells were CD8+ and their subpopulation DR+. The cells had strong spontaneous and inducible suppressor functions in vitro.