Targeted genotyping-by-sequencing of potato and data analysis with R/polyBreedR.

Mid-density targeted genotyping-by-sequencing (GBS) combines trait-specific markers with thousands of genomic markers at an attractive price for linkage mapping and genomic selection. A 2.5K targeted GBS assay for potato (Solanum tuberosum L.

Early-Stage Phenotyping of Sweet Potato Virus Disease Caused by Sweet Potato Chlorotic Stunt Virus and Sweet Potato Virus C to Support Breeding.

Sweet potato virus disease (SPVD) is a global constraint to sweetpotato () production, especially under intensive cultivation in the humid tropics such as East Africa. The objectives of this study were to develop a precision SPVD phenotyping protocol, to find new SPVD-resistant genotypes, and to standardize the first stages of screening for SPVD resistance. The first part of the protocol was based on enzyme-linked immunosorbent assay results for sweet potato chlorotic stunt virus (SPCSV) and sweet potato virus C (SPVC) with adjustments to a negative control (uninfected clone Tanzania) and was performed on a prebreeding population (VZ08) comprising 455 clones and 27 check clones graft inoculated under screenhouse conditions.

Cytoplasmic Male Sterility Incidence in Potato Breeding Populations with Late Blight Resistance and Identification of Breeding Lines with a Potential Fertility Restorer Mechanism.

Cytoplasmic male sterility (CMS) in potato is a common reproductive issue in late blight breeding programs since resistant sources usually have a wild cytoplasmic background (W or D). Nevertheless, in each breeding cycle male fertile lines have been observed within D- and T-type cytoplasms, indicating the presence of a fertility restorer () mechanism. Identifying sources of and complete male sterility to implement a CMS- system in potato is important since hybrid breeding is a feasible breeding strategy for potato.

QTL mapping and validation of fertility restoration in West African sorghum A cytoplasm and identification of a potential causative mutation for Rf.

Key message Major A cytoplasm fertility restoration loci, Rf and Rf , were found in the West African sorghum. A potential causative mutation for Rf was identified. KASP markers were validated on independent material.