An efficient approach for overproduction of DNA polymerase from Pyrococcus furiosus using an optimized autoinduction system in Escherichia coli.

High fidelity DNA polymerase from Pyrococcus furiosus (Pfupol) is an attractive alternative to the highly popular DNA polymerase from Thermus aquaticus. Because this enzyme is in great demand for biotechnological applications, optimizing Pfupol production is essential to supplying the industry's expanding demand. T7-induced promoter expression in Escherichia coli expression systems is used to express recombinant Pfupol; however, this method is not cost-effective.

High-level expression of codon-optimized Taq DNA polymerase under the control of rhaBAD promoter.

A DNA polymerase from Thermus aquaticus remains the most popular among DNA polymerases. It was widely applied in various fields involving the application of polymerase chain reaction (PCR), implying the high commercial value of this enzyme. For this reason, an attempt to obtain a high yield of Taq DNA polymerase is continuously conducted.

Optimized expression of large fragment DNA polymerase I from in expression system.

DNA polymerase is a DNA polymerase derived from has a strand-displacement activity, and is used in loop-mediated isothermal amplification (LAMP) for rapid detection of COVID-19. Despite its potential to be employed in the detection of COVID-19, using commercially available enzymes is not economically feasible. The use of noncommercial enzyme for routine use is desirable.

Viral and Host Factors are Related to the Progression of HIV Diseases in Mimika, Papua.

Background: Papua has a high cumulative number of HIV, which has expanded epidemic status with the most risk factors are heterosexuals.

Aim: This study aims to determine factors associated with HIV disease progression include host and viral factors.

Methods: Eighty-four subjects recruited in Rumah Sakit Mitra Masyarakat (RSMM) VCT's laboratory, interviewed with questionnaires and also did laboratory examinations.