Publications by authors named "Moacir F De Oliveira"

Collared Peccary (Pecari tajacu, Linnaeus, 1758) is a mammalian Tayassuidae species from tropical to semi-arid areas. The morphological features of the oral cavity in this species were identified and described. Tonsils are secondary lymphoid organs essential for contact with antigens due to food and air intake.

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Considering the importance of efficiently obtaining somatic resource banks as an ex-situ conservation strategy for wild mammals, we evaluated two techniques (slow freezing - SF and solid-surface vitrification - SSV) for the cryopreservation of ear cartilage and skin from six-banded armadillos. Additionally, we analyzed the effects of two combinations of intracellular cryoprotectants (3.0 M or 6.

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  • The study assessed how different culture media and concentrations of GDNF affect the in vitro culture of testicular tissues from prepubertal collared peccaries over 28 days.
  • Using Dulbecco's modified essential medium (DMEM) and StemPro-34 SFM, researchers found that the StemPro medium with 10 ng/mL GDNF led to better morphology scores compared to DMEM.
  • While cell viability remained consistent across treatments (~70%), only DMEM plus 10 ng/mL GDNF notably improved cellular proliferation by Days 14 and 28, indicating the importance of GDNF for cell survival and growth in cultured testicular tissue.
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  • The study focused on the external morphology of collared peccary embryos and fetuses to improve understanding of gestational development for assisted reproduction technologies.
  • A total of 22 animals were examined at various gestational ages (25-145 days post-copulation), allowing researchers to document key developmental stages and anatomical features.
  • Results indicated significant changes across three gestational phases, from early curvature and initial organ formation to full organ development and notable changes in skin and dental features, contributing to comparative anatomy knowledge for better reproductive management.
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While titanium dental implants have already been clinically established, ongoing research is continuously being conducted to advance the fields of osseointegration and bacterial resistance, seeking further improvements in these areas. In this study, we introduce an innovative method for treating titanium surfaces within tightly sealed packaging. Specifically, titanium discs, enclosed in surgical-grade packaging, underwent treatment using cold atmospheric plasma (CAP).

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  • The study focused on identifying sexual dimorphism in stillborn hawksbill sea turtles by examining the morphological differences in their gonads using various microscopy techniques.
  • Female gonads were characterized by a spindle shape and a grainy texture, while male gonads were ovoid and had a smooth surface, indicating distinct structural differences.
  • Microscopic and ultrastructural analyses revealed unique cellular arrangements in females with oocytes and blood vessels, compared to males, which had testicular cords and distinct cell types, confirming these evaluation methods are reliable for sex determination in these turtles.
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  • - The study aimed to evaluate various aspects of spermatozoa in the greater rhea, including their movement, viability and membrane integrity, as well as differences between sperm from the epididymis and vas deferens.
  • - Semen samples from 7 adult rheas showed that the sperm had an average motility of 14.6%, with viability rates varying between 64.6% and 72.1% depending on the staining method used.
  • - The research established new reference values for evaluating rhea sperm, which can aid in improving sperm handling and freezing methods for this species.
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Morphological study of the tongue is an interesting way of understanding evolutionary processes associated with feeding habits. Therefore, the aim of the present study was to describe the tongue morphology of the Antillean manatee and to understand possible morphological relationships with its way of capturing food. Macroscopic dissections and light and scanning electron microscopy analyses of seven manatee tongues were performed.

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Sexual differentiation and steroidogenic mechanisms have an important impact on postnatal gonadal phenotypic development. Thus, establishing the activities that lead to male phenotypic development can provide a better understanding of this process. This study examined the prenatal development of cavies to establish morphological and histometric development patterns and protein and enzyme immunolocalization processes that are responsible for androgen synthesis in the testes and epididymis.

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Background: Hystricomorpha rodents display a similar placentation model to humans. The present study was carried out considering the scarcity of information concerning the placental development in agouti.

Objective: Describe the microscopy of the placenta, subplacenta and yolk sac of agoutis in early pregnancy and report on the inversion of the yolk sac.

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Understanding the cardiovascular system is fundamental in diagnosing pathologies and interpreting exams, such as contrast radiographs. In this context, the present study describes the collateral abdominal aorta artery branches of red-rumped agouti. Ten red-rumped agoutis, six males and four females, were assessed.

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Although oocyte in vitro maturation (IVM) is routinely used for in vitro embryo production in mice and rats, its use in wild rodents remains unexplored. Evidence suggests that hormone and growth factor supplementation influence oocyte meiotic resumption. This study evaluated the synergistic effects of follicle-stimulating hormone (FSH) and epidermal growth factor (EGF) on the IVM and parthenogenetic development of red-rumped agouti oocytes.

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  • The greater rhea, a significant wild ratite in Brazil, is important for poultry research focusing on productivity enhancements.
  • Current studies on its fetal attachments and embryonic development are essential for improving reproductive and nutritional management in these animals.
  • This study aimed to standardize the model for fetal attachments in greater rhea, revealing that its embryonic development patterns closely resemble those of other bird species.
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Microbial resistance to drugs is a public health problem; therefore, there is a search for alternatives to replace conventional products with natural agents. One of the potential antimicrobial agents is wood vinegar derived from the carbonization of lignocellulosic raw materials. The objectives of the present work were to evaluate the antibacterial and antifungal action of two kinds of wood vinegar (WV), one of Eucalyptus urograndis wood and another of Bambusa vulgaris biomass, and determine their chemical profile.

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Estrogens and progestogens are hormones produced by maternal organs and it is required for recognition and maintenance of pregnancy. In addition, the embryo may also be a source. For this, the aim was to identify steroidogenic expression on Galea spixii embryos early in the embryonic period.

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Duchenne Muscular Dystrophy (DMD) reproductive alterations and the influence of antioxidant treatments may aid in understanding morphometry testicular quantification. In this context, the aim of the present study was to characterize the intertubular compartment (ITC) morphometry of animal testes in mdx mice supplemented with ascorbic acid (AA). Sixteen mice were used, namely the C57BL/10 (non-dystrophic) and C57BL/10Mdx (dystrophic) lineages, distributed into the following groups: Control (C60), Dystrophic (D60), Control supplemented with AA (CS60), Dystrophic supplemented with AA (DS60).

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  • * The study investigated the presence and characteristics of steroidogenic cells in the mesonephros of turtle embryos, specifically examining their structure through various microscopy techniques.
  • * Results revealed that these cells display features typical of steroid-producing cells, indicating that the mesonephros may have a key role in the gonadal differentiation of the turtle species P. expansa.
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The determination of arterial vascular distribution patterns can contribute to more detailed knowledge on arterial systems. In this context, the aim of the present study was to describe the collateral aortic branches in the collared peccary, aiming to define a standard model for this species, providing information through comparative anatomy for analysis of some evolutionary aspects of the order Artiodactyla. Ten young male animals were employed.

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We studied the sperm membrane functionality through the epididymal transit by comparing different hypoosmotic solutions and verifying possible associations among osmotic response and functional parameters of sperm in red-rumped agouti (Dasyprocta leporina). For this purpose, epididymal sperm from six sexually mature male agoutis were collected via flotation. Then, analyses of sperm parameters and hypoosmotic swelling test using different hypoosmotic solutions (0, 50 and 200 mOsm/L) in different regions of the epididymis (caput, corpus and cauda) were performed.

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Heterologous fertilization (IVF) is an important tool for assessing fertility of endangered mammals such as the jaguar, considering difficult access to females for artificial insemination and to obtain homologous oocytes. We aimed to evaluate the fertility of jaguar sperm cryopreserved with different extenders, using domestic cat oocytes to assess the development of hybrid embryos. Semen from four captive jaguars was obtained by electroejaculation.

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Aims: Hyperbaric oxygen (HBO) therapy has been widely used for the adjunctive treatment of diabetic wounds, and is currently known to influence left ventricular (LV) function. However, morphological and molecular repercussions of the HBO in the diabetic myocardium remain to be described. We aimed to investigate whether HBO therapy would mitigate adverse LV remodeling caused by streptozotocin (STZ)-induced diabetes.

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Background: Cardiomyocyte size histomorphometry is widely required in myocardial remodeling research, but it relies on subjective, time-consuming manual tracing. We aimed to present the CmyoSize, a Fiji/ImageJ macro that reproducibly measures the transnuclear cross-sectional size of cardiomyocytes in multiple H&E images.

Methods: CmyoSize output per image are: mean cross-sectional area (CSA), CSA's local standard deviation (LSD), mean width, and width's LSD.

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Letrozole is used as a therapeutic agent in reproductive disorders caused by high estrogen levels. Letrozole inhibits cytochrome P450 aromatase and reduces estrogen levels. However, the effects of long-term use on reproductive traits are unknown.

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Background: The placenta of hystricomorph rodents, lagomorphs and some primates includes an unusual structure, termed a subplacenta, which essentially consists of trophoblastic cells located deep to the central implantation site within the area of decidualization. It has been suggested that the subplacenta is functionally important, although considerable controversy remains on the issue. In this context, our objective was to compare the architecture and structure of the subplacentas of different hystricomorph species, to investigate the possibility that it is active in hormone synthesis.

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The palate is a fundamental region in food swallowing and presents different adaptations in species. This research aimed to describe structural and ultrastructural characteristics of the palatine epithelium and the connective tissue cores (CTCs) of ten red-rumped agoutis (Dasyprocta leporina—Linnaeus, 1758) using macroscopic, light microscopy, scanning electron microscopy, and transmission electron microscopy. We found nine palatine ridges in the diastema and hard palate, and a smooth surface in the soft palate.

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