Development and Validation of the 9-Dye Fluorescent Autosomal and Y Chromosome Multiplex STR Amplification System.

To develop a 9-dye fluorescent autosomal and Y chromosome STR multiplex amplification system and verify its performance. Using the 9-dye fluorescent labeling technology, construct a multiplex amplification system that uses the autosomal and Y chromosome core and preferred loci recommended by GB/T 41009-2021 national standard as candidate loci, then evaluate its accuracy, sensitivity, species specificity, and other system performance index. A 9-dye fluorescent multiplex amplification system containing 70 loci, including Amel, D5S818, D21S11, D7S820, CS1FPO, D2S1338, D19S433, D8S1132, D2S441, VWA, D8S1179, D16S539, D10S1435, PentaD, D3S3045, TPOX, TH01, D22S1045, D18S51, FGA, D19S253, D10S1248, D6S477, D6S1043, D13S317, D12S391, D15S659, PentaE, D1S1656, D3S1358, DYS460, DYS389I, DYS390, DYS533, DYS392, DYS518, DYS557, DYS570, DYS458, DYS437, DYS385, GATA-H4, DYS576, DYS643, DYS508, DYS627, DYS456, DYS391, DYF404S1, DYS438, DYS448, DYF387S1, DYS596, DYS549, DYS399S1, DYS393, DYS635, DYS439, DYS19, DYS444, DYS449, DYS481, DYS527a/b, and DYS447 was successfully constructed.

Effect of Interaction of ATG7 and Plasma Metal Concentrations on Cognitive Impairment in Rural China.

The objective of this study is to explore the association of plasma metal concentrations with impaired cognitive function in different genotypes of ATG7 using multiple models. A cross-sectional survey was conducted in rural China among 994 individuals aged 30 years or older. Cognitive function was assessed using the Mini-Mental State Examination (MMSE).

Association Between Urinary Metal Levels and Chronic Kidney Dysfunction in Rural China: A Study on Sex-Specific Differences.

Background: While current epidemiological studies have documented associations between environmental metals and renal dysfunction, the majority have concentrated on plasma metal levels. The relationship between urinary metal exposure and chronic kidney disease (CKD) remains contentious, particularly within specific demographic groups.

Methods: This cross-sectional study included 2919 rural Chinese adults recruited between 2018 and 2019.

Correlations between growth differentiation factor 15 (GDF-15) serum levels and gene polymorphism with type 2 diabetes mellitus.

Purpose: To date, the relationship between Growth Differentiation Factor 15 (GDF-15) gene polymorphism and the risk of type 2 diabetes mellitus (T2DM) has not been clarified. Our study aims to explore the association between serum GDF-15 levels and related gene polymorphism with the risk of T2DM in a Chinese rural Yao population.

Methods: This was a 1:1 case-control study with 179 T2DM patients and 179 age- and sex-matched control participants.

Association between multiple-heavy-metal exposures and systemic immune inflammation in a middle-aged and elderly Chinese general population.

Background: Exposure to heavy metals alone or in combination can promote systemic inflammation. The aim of this study was to investigate potential associations between multiple plasma heavy metals and markers of systemic immune inflammation.

Methods: Using a cross-sectional study, routine blood tests were performed on 3355 participants in Guangxi, China.

Genetic overlap and causal inferences between diet-derived antioxidants and small-cell lung cancer.

Several studies have reported that antioxidants exert both preventive and inhibitory effects against tumors. However, their causal effects on small-cell lung cancer (SCLC) remain controversial. Herein, we explored the causal effects of 6 antioxidants on SCLC by combining a genome-wide association study database and the Mendelian randomization (MR) approach.

Association Between Combined Polymetallic Exposure and Osteoporosis.

Combined polymetallic exposure may be an influential factor in osteoporosis. This study aimed to explore the association between polymetallic combined exposure and osteoporosis. A total of 2115 participants were included.

Association between multiple metals exposure and sleep disorders in a Chinese population: A mixture-based approach.

Objective: Previous studies have suggested a possible association between metals and sleep disorders. This study aimed to explore the association between Zn, Cu, Se, Mg and Ca and sleep disorders in single and multi-metal co-exposure models.

Methods: Logistic regression models, restricted cubic spline model (RCS), Quantile g computation (Q-gcomp), Weighted Quantile Sum (WQS), and Bayesian kernel machine regression (BKMR) models were used to investigate the association between metal levels and sleep disorders.

ApoE gene polymorphisms and metals and their interactions with cognitive function.

Objective: To analyze the relationship between plasma metal elements, ApoE gene polymorphisms and the interaction between the two and impaired cognitive function in elderly population.

Method: A stratified sample was drawn according to the age of the study population, and 911 subjects were included. Baseline information and health indicators were obtained, and cognitive function status was assessed by health examination, a general questionnaire and Mini-Mental Status Examination.

Association between plasma and dietary trace elements and obesity in a rural Chinese population.

Trace elements may play an important role in obesity. This study aimed to assess the plasma and dietary intake levels of four trace elements, Mn, Cu, Zn and Se in a rural Chinese population, and analyse the relationship between trace elements and obesity. A cross-sectional study involving 2587 participants was conducted.

Association between barium exposed, CYP19A1 and central obesity: A cross-sectional study in rural China.

Background: obesity is a major risk factor for many metabolic diseases such as diabetes and cardiometabolic diseases. This study aimed to evaluate the association of plasma and urinary barium concentrations, CYP19A1 gene polymorphisms, and their interaction with central obesity in a rural Chinese population.

Methods: restricted cubic spline model was used to explore the dose-response relationship between barium and the risk of developing central obesity and waist circumference; logistic regression model was used to assess the association between barium, CYP19A1 gene polymorphisms and their interaction with central obesity.

Association between combined exposure to plasma heavy metals and dyslipidemia in a chinese population.

Background: Exposure to heavy metals in the environment is widespread, while the relationship between combined exposure to heavy metals and dyslipidemia is unclear.

Methods: A cross-sectional study was performed, and 3544 participants aged 30 years or older were included in the analyses. Heavy metal concentrations in plasma were based on inductively coupled plasma‒mass spectrometry.

Association of CYP19A1 Gene, Plasma Zinc, and Urinary Zinc with the Risk of Type 2 Diabetes Mellitus in a Chinese Population.

To explore the effects of CYP19A1 gene polymorphisms, plasma zinc, and urinary zinc levels and their interactions on type 2 diabetes mellitus (T2DM) in residents of Gongcheng County, Guangxi, China. The case-control study was used for the investing. The MassARRAY System was applied to genotype the CYP19A1 genes rs752760, rs10046, rs10459592, and rs700518 in 540 study subjects.

Associations of Dietary and Plasma Copper Levels with Liver Function in a Chinese Population.

The potential mechanisms underlying the association between copper (Cu) exposure and impaired liver function are unclear. This study aimed to investigate the potential associations of dietary Cu intake and plasma Cu levels with liver function biomarkers. A cross-sectional study was performed to assess liver function biomarkers-namely, levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), alanine transaminase (ALT), and aspartate transaminase (AST)-in 2376 subjects in Guangxi, China.

JAK2/STAT3 Signaling Pathway and Klotho Gene in Cadmium-induced Neurotoxicity In Vitro and In Vivo.

Cadmium (Cd), a common heavy metal in the environment, is associated with cognitive impairment. In the present study, we carried out a preliminary inquiry to explore whether Cd causes neurotoxicity by regulating the JAK2/STAT3 signaling pathway and affecting the expression of klotho genes in vivo and in vitro, providing clues for the mechanism of Cd-induced cognitive dysfunction. The rat samples were injected with Cd chloride solution for 14 weeks, and the memory function of the rats was detected.

Sex-Specific Associations of Urinary Metals with Renal Function: a Cross-sectional Study in China.

Background: Extensive studies have revealed the link between heavy metals and CKD. Compared to single meta-elements, mixture of metals reflect real-life metals exposure scenarios and are of interest. However, the mechanism of action of metal mixture on renal function is unclear.

Association of Dietary and Plasma Magnesium with Glycaemic Markers in a Chinese Population.

Certain metals play a role in the pathogenesis of diabetes. This study aimed to investigate the potential association of plasma magnesium (Mg) and dietary intake of Mg with glycaemic markers. A cross-sectional study was conducted on 2373 subjects in Guangxi, China.

Associations of Gene Polymorphisms With Cognitive Function in Rural Chinese Population.

The study aimed to investigate the relationship between transcription factor EB () gene polymorphisms, including their haplotypes, and the cognitive functions of a selected population in Gongcheng County, Guangxi. A case-control study approach was used. The case group comprised 339 individuals with cognitive impairment, as assessed by their Mini-Mental State Examination scores; the control population also comprised 339 individuals who were matched by sex and age (± 5 years) in a 1:1 ratio.

Correlation between urinary contents of some metals and fasting plasma glucose levels: A cross-sectional study in China.

Many metals are involved in the pathogenesis of diabetes, but most of existing studies focused on single metals. The study of mixtures represents real-life exposure scenarios and deserves attention. This study aimed to explore the potential relationship of urinary copper (Cu), zinc (Zn), arsenic (As), selenium (Se), and strontium (Sr) contents with fasting plasma glucose (FPG) levels in 2766 participants.

Associations Between Plasma Metals and Cognitive Function in People Aged 60 and Above.

The objective of the study was to explore the relationship between the plasma levels of 22 metals and cognition status in older adults aged 60 years and above. A cross-sectional survey was conducted between 2018 and 2019. Inductively coupled plasma mass spectrometry (ICP-MS) was used to detect the concentrations of metals, and a mini-mental state examination (MMSE) questionnaire was used to estimate the cognition status of the elderly.

Strategy for Producing the High-Quality Glycopeptide Antibiotic A82846B in Based on the CRISPR-Cas12a System.

Oritavancin is a new-generation semisynthetic lipoglycopeptide antibiotic used to prevent the spread of vancomycin-resistant Gram-positive bacteria. The glycopeptide A82846B is the direct precursor of oritavancin. Considering the structural similarity between A82846B and vancomycin, the vancomycin producer was used as a chassis for the construction of a strain producing high-quality A82846B.

Associations between multiple heavy metals exposure and glycated hemoglobin in a Chinese population.

Background: Heavy metals may play an important role as environmental risk factors in diabetes mellitus. This study aimed to explore the association of HbA1c with As, Cd, Cu, Ni, Pb, and Zn in single-metal exposure and multi-metal co-exposure models.

Methods: A cross-sectional study involving 3472 participants was conducted.

A novel multiplex of 12 multicopy Y-STRs for forensic application.

Y chromosomal short tandem repeats (Y-STRs) have been applied overwhelmingly in forensic areas for solving paternity identification and sexual assault cases. Yet the widely used Y-STR kits contain mostly single-copy markers, which may restrict the discrimination power. Here, a novel Y-STR multiplex was developed and validated in order to complement the currently available Y-STR kits, especially on differentiating male relatives.

Development and validation of a new 18 X-STR typing assay for forensic applications.

With a unique inheritance pattern compared to autosomal short tandem repeats (A-STRs), X chromosomal STRs (X-STRs) have special usage in forensic relationship testing. In this study, we designed a multiplex amplification system (named TYPER-X19 multiplex assay) consisting of 18 STR loci spreading from 7.837 to 149.