Finding the ideal solvent for the analysis of polar analytes using supercritical fluid chromatography.

The analysis of polar analytes with the help of hydrophilic interaction liquid chromatography (HILIC) using classic methods of high-performance liquid chromatography is not without its downsides. In these applications, acetonitrile is prevalent as main eluent and sample diluent. This results not only in slow diffusion processes during the separation, but also in often unstable sample solutions where polar analytes are concerned.

Application of chiral stationary phases for the separation of vitamin A acetate isomers.

The separation of vitamin A acetate isomers is essential for quality assurance of e.g. nutrition supplements, cosmetics, and pharmaceutical ingredients.

Selectivity optimization in liquid chromatography via stationary phase tuning.

In striving for the best possible separation, the selectivity of stationary phases as an optimization parameter is often underestimated although there are many ways to influence this powerful tool. This review serves to provide an insight into the various ways of adapting the selectivity of a separation in liquid chromatography. Approaches via temperature and flow rate tuning are discussed as a basis followed by focusing on the stationary phase as the superior optimization parameter.

Performance in (Ultra-)high-performance liquid chromatography-How to qualify and optimize instruments in practice.

This paper investigates the suitability of an ultra-high-performance liquid chromatography/high-performance liquid chromatography hybrid system for ultra-high-performance liquid chromatography applications. Thus, the effect of extra column band broadening, the gradient system, and the injection system were tested and optimized according to their capabilities. An increase of the theoretical plate number up to a factor of two is achieved by the optimization of the extra column volume into the typical ultra-high-performance liquid chromatography range (<10 μl).