Effects of soft tissue punch size on the healing of peri-implant tissue in flapless implant surgery.

Objective: In an attempt to help produce guidelines for the use of soft tissue punches, this animal study was undertaken to examine the effects of soft tissue punch size on the healing of peri-implant tissue in a canine mandible model.

Study Design: Bilateral, edentulous, flat alveolar ridges were created in the mandibles of 6 mongrel dogs. After a 3-month healing period, 3 fixtures (diameter 4.

Prevalence of metabolic syndrome in adult hypopituitary growth hormone (GH)-deficient patients before and after GH replacement.

Context And Objective: Metabolic and body compositional consequences of GH deficiency (GHD) in adults are associated with a phenotype similar to the metabolic syndrome (MetS).

Patients: We assessed MetS prevalence in adult GHD patients (n = 2531) enrolled in the Hypopituitary Control and Complications Study. Prevalence was assessed at baseline and after 3 yr of GH replacement in a subset of 346 adult-onset patients.

Characterization and promoter activity analysis of a new porcine gene: NICE-3.

Through comparative gene mapping, NICE-3, which is closely linked to tropomyosin 3 in human chromosome 1, was selected to be investigated as a new candidate gene associated with the muscle development in pigs. This gene was sequenced, chromosome mapped, expression analyzed, subcellularly localized, and promoter activity analyzed. After screening and sequencing, porcine NICE-3 was found in a bacterial artificial chromosome clone containing tropomyosin 3.

Increased vascular permeability precedes cellular inflammation as asthma control deteriorates.

Background: Airway microcirculation is abnormal in asthma but the role of vascular changes in asthma deteriorations remains poorly defined. We prospectively assessed the vascular changes accompanying worsening of asthma control by using an inhaled corticosteroid (ICS) dose-reduction model.

Objectives: To evaluate airway vascularity, vascular permeability and expression of vascular endothelial growth factor (VEGF) in early asthma deterioration induced by ICS back-titration.

Treatment of recurrent miscarriage and antiphospholipid syndrome with low-dose enoxaparin and aspirin.

The aim of this retrospective, observational study was to determine the impact of low-dose enoxaparin (20 mg) in conjunction with low-dose aspirin on the pregnancy outcome of women with antiphospholipid syndrome and recurrent miscarriage. The study was conducted in a tertiary referral teaching hospital. A total of 35 women with antiphospholipid syndrome were treated with low-dose enoxaparin and aspirin as soon as pregnancy was confirmed.

Molecular cloning, expression patterns and subcellular localization of porcine TMCO1 gene.

The product of transmembrane and coiled-coil domains 1 (TMCO1) gene is a member of DUF841 superfamily of several eukaryotic proteins with unknown function. The partial DNA sequence of porcine TMCO1 was first cloned with a pig 567 bp ORF encoding 188 amino acids. By tissues expression analysis, the TMCO1 was found highly expressed in the liver, kidney and heart.

Sequence identification, chromosomal mapping and tissue specific expression of the porcine paraoxonase 1 (PON1) gene.

Serum paraoxonase (PON1) plays an important role in protecting low-density lipoprotein against oxidative modification. In this study, the full-length cDNA sequence (1,416 bp) of porcine PON1 was cloned by reverse transcription polymerase chain reaction and rapid amplification of cDNA ends. It was found to contain a 1,068 bp open reading frame encoding a deduced protein of 355 amino acids with a calculated molecular weight of 40.

Cloning and characterization of the 5'-flanking region of the pig adiponectin gene.

Adiponectin, a cytokine hormone secreted exclusively by adipose tissue, has key roles in energy homeostasis, and in glucose and lipid metabolism. To understand the regulatory expression of pig adiponectin, the 5'-flanking region of the adiponectin gene was isolated from a pig BAC library. 5'-RACE analysis revealed that there were three transcriptional start sites, including one that is novel.

[A report of 463 in-hospital cardiopulmonary resuscitation based on the "Utstein Style"].

Objective: Assessment of outcomes and outcome-related factors of in-hospital cardiopulmonary resuscitation (CPR) based on the "Utstein Style".

Methods: The study was designed as a prospective, single-institution, registry investigation of 463 patients (included adult and pediatric patients) for whom a CPR was attempted.

Results: The study population consisted of 320 (69.

[Research progress of nanosecond pulsed electric field applied to intracellular electromanipulation].

In recent years, many experts have done some researches on experiment and mechanism of intracellular electromanipulation (IEM) under nanosecond pulsed electric field (nsPEF). The experiment results have shown that nsPEF could not induce electroporation of cell membrane, but could induce intracellular effects such as apoptosis, calcium release, enhancement of gene expression, and fragmentation of DNA and chromosome. In order to account for the phenomenon, researchers believe that when the pulse width of the pulsed electric field is larger than the charging time of plasma membrane, the pulsed electric field mainly targets on the outer membrane of cell; and that the effect of the pulsed electric field on nucleus and nuclear membrane increases with the decrease of the pulse width.

Changing patterns of the adult growth hormone deficiency diagnosis documented in a decade-long global surveillance database.

Background: GH therapy in adult patients with GH deficiency (GHD) was approved over 10 yr ago, and the indication has subsequently gained broad acceptance. The HypoCCS surveillance database is a suitable means to examine the evolution of diagnostic patterns since 1996.

Methods: Baseline demographics, reported cause of GHD, and diagnostic tests were available from 5893 GH-treated patients.

Palladacycle-catalyzed highly efficient kinetic resolution of 1-hydroxy-2-aryl-1,2-dihydronaphthalenes via dehydration reaction.

Palladacycles showed their high efficiency in the kinetic resolution of 1-hydroxy-2-aryl-1,2-dihydronaphthalenes via dehydration, providing optically active products in high yields and high ee with an S factor up to 26. The superiority of a benzylic-substituted palladacycle in asymmetric induction was also demonstrated.

Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent omegaG binding to a group I intron.

Self-splicing of group I introns is accomplished by two sequential ester-transfer reactions mediated by sequential binding of two different guanosine ligands, but it is yet unclear how the binding is coordinated at a single G-binding site. Using a three-piece trans-splicing system derived from the Candida intron, we studied the effect of the prior GTP binding on the later omegaG binding by assaying the ribozyme activity in the second reaction. We showed that adding GTP simultaneously with and prior to the esterified omegaG in a substrate strongly accelerated the second reaction, suggesting that the early binding of GTP facilitates the subsequent binding of omegaG.

Inactivation of the CYLD deubiquitinase by HPV E6 mediates hypoxia-induced NF-kappaB activation.

The biochemical mechanisms that underlie hypoxia-induced NF-kappaB activity have remained largely undefined. Here, we find that prolonged hypoxia-induced NF-kappaB activation is restricted to cancer cell lines infected with high-risk human papillomavirus (HPV) serotypes. The HPV-encoded E6 protein is necessary and sufficient for prolonged hypoxia-induced NF-kappaB activation in these systems.

[Effect of Tween 80 and rhamnolipid on enzymatic hydrolysis of straw].

The method of enzymatic hydrolysis was adopted and straw was used as substrate to study the effects of reducing sugar yield, enzyme stability and convention rate of cellulose during the course of enzymatic hydrolysis as well as the impacts of enzyme kinetic characteristics and adsorption of cellulase on cellulose by addition of chemical-surfactant Tween 80 and biosurfactant rhamnolipid. The results indicated that Tween 80 and rhamnolipid at different concentrations enhanced the production of reducing sugar. Tween 80 at 0.

Pomegranate extract inhibits androgen-independent prostate cancer growth through a nuclear factor-kappaB-dependent mechanism.

Constitutive nuclear factor-kappaB (NF-kappaB) activation is observed in androgen-independent prostate cancer and represents a predictor for biochemical recurrence after radical prostatectomy. Dietary agents such as pomegranate extract (PE) have received increasing attention as potential agents to prevent the onset or progression of many malignancies, including prostate cancer. Here, we show that PE inhibited NF-kappaB and cell viability of prostate cancer cell lines in a dose-dependent fashion in vitro.

Asymmetric ring-opening reaction of oxabicyclic alkenes with aryl boronic acids catalyzed by P-containing palladacycles.

The chiral phosphine-containing palladacycle, synthesized easily from H-MOP, showed its high catalytic activity as well as asymmetric induction ability in ring-opening reaction of oxabicyclic alkenes with arylboronic acids, providing corresponding products in high yields and high ee.

Molecular characterization and expression analysis of caveolin-1 in pig tissues.

Members of the caveolin family played important roles during fundamental cellular processes, such as regulation of cell morphology, migration, and gene expression in muscle cells. In this study, caveolin-1 (Cav-1), one of the caveolins, was identified from longissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display analysis. The deduced amino acids sequence of the porcine Cav-1 contained a caveolin domain, and was very conservative among different species.

Characterization, expression profiles, intracellular distribution and association analysis of porcine PNAS-4 gene with production traits.

Background: In a previous screen to identify differentially expressed genes associated with embryonic development, the porcine PNAS-4 gene had been found. Considering differentially expressed genes in early stages of muscle development are potential candidate genes to improve meat quality and production efficiency, we determined how porcine PNAS-4 gene regulates meat production. Therefore, this gene has been sequenced, expression analyzed and associated with meat production traits.

[Effects of stem numbers per ground area on the quality of standard cut Chrysanthemum morifolium in greenhouse: simulation model].

In order to understand the effects of stem numbers per ground area on the quality of standard cut Chrysanthemum morifolium, an experiment with different cultivars, different stem numbers per plant, different planting densities, and different planting dates was conducted in a greenhouse in Shanghai in 2005 and 2006. The effects of stem numbers per ground area on the canopy leaf area index and external quality of standard cut C. morifolium were quantified using the experimental data.

Cloning and characterization of class 1 and class 2 insulin-like growth factor-I mRNA in Songliao black pig.

Insulin-like growth factor-I (IGF-I) plays a fundamental role in postnatal mammalian growth, development, and metabolism. The mammalian IGF-I gene contains at least six exons from which several different mRNAs are transcribed. In this study, IGF-I mRNA in Songliao black pig liver was investigated using RLM-RACE.

Effects of steep pulsed electric fields (SPEF) on mitochondrial transmembrane potential of human liver cancer cell.

Mitochondrial plays an important role in apoptosis, and measuring the change of mitochondrial transmembrane potential (delta psi m) is a useful method for apoptosis. Exposing human liver cancer cell SMMC-7721 dyed with Rhodamine 123 to steep pulsed electric fields (SPEF), this paper observed the real-time change of delta psi m using laser scanning confocal microscope (LSCM) to study the apoptosis effect of SPEF. The experiment results showed that delta psi m decreased gradually in process of exposing to SPEF, SPEF with higher voltage (600V) and shorter width (100ns) could cause a quicker decrease than SPEF with lower voltage (200V) and longer width (1.

[Temporal and spatial variation of MODIS vegetation indices in Hunan Province].

Based on MODIS images and by using the algorithm of maximum value composite (MVC), the monthly vegetation indices (VIs) in 2005 in Hunan Province were obtained. Through the analysis of the MODIS VIs, Hunan Province was divided into six districts to describe the spatial distribution of the VIs, and by using the monthly mean temperature and rainfall data collected from 5 climatic monitoring stations in this province, the temporal variation of the VIs was analyzed. The results showed that the spatial distribution of MODIS VIs was positively correlated with vegetation cover, and appeared regional characteristics.

Comparative molecular characterization of ADSS1 and ADSS2 genes in pig (Sus scrofa).

Adenylosuccinate synthetase (ADSS) catalyzes the key step of AMP synthesis. Vertebrates have two isozymes of ADSS, which are named ADSS1 and ADSS2, respectively. In this study, we cloned porcine ADSS1 and ADSS2 genes and comparatively analyzed their sequence, chromosome mapping, mRNA distribution and subcellular localization.