Reaction mechanism of mRNA guanylyltransferase from rat liver: isolation and characterization of a guanylyl-enzyme intermediate.

Rat liver RNA guanylyltransferase catalyzes a GTP-PPi exchange reaction in the absence of acceptor RNA [Mizumoto, K. & Lipmann, F. (1979) Proc.

Pulmonary function before and after abdominal surgery in the aged.

In patients 70 years or older, pulmonary function tests were performed before and after abdominal surgery to correlate the results with the development of postoperative pulmonary complications which developed in 48% of these patients, compared to 15% in the control group. To predict the development ot PPC, preoperative analysis of the flow-volume curve is useful and 4 (V50-V25)/forced vital capacity is a valuable parameter for the analysis of the flow-volume curve. Postoperatively, pulmonary function was reduced and there was a delay in the restoration of pulmonary function in cases with postoperative pulmonary complications.

Carcinosarcoma of the esophagus--a case report.

We report a case of true esophageal carcinosarcoma, in which well-differentiated squamous cell carcinoma and spindle-shaped cell sarcoma were intermingled in the primary and metastatic lesions.

Transmethylation and transguanylylation in 5'-RNA capping system isolated from rat liver nuclei.

Rat liver nuclei were isolated and sonicated for extraction in order to study the capping of RNA. The guanosine 7-methyltransferase was purified from the extract by hydroxylapatite column chromatography with stepwise addition of phosphate buffer. It was assayed by using as methyl acceptor synthetic G(5')ppp(5')G and S-adenosylmethionine as donor.