Publications by authors named "Mizuki Fujisawa"
High-throughput isolation of SARS-CoV-2 nucleocapsid antibodies for improved antigen detection.
Biochem Biophys Res Commun
September 2023
Article Synopsis
- SARS-CoV-2 nucleocapsid protein (NP) is crucial for COVID-19 diagnostic tests like PCR and antigen rapid diagnostic tests (Ag-RDTs).
- Ag-RDTs are more user-friendly than PCR, allowing for easier point-of-care and self-testing to detect the virus.
- The study identified two high-affinity antibodies targeting distinct sites on NP, improving the sensitivity and specificity of Ag-RDTs, showcasing the effectiveness of high-throughput antibody isolation methods for enhancing diagnostic tools.
Article Synopsis
- * A new diagnostic method called TLip-LISA uses temperature-responsive liposomes to detect SARS-CoV-2 proteins quickly and effectively, replacing previous methods that required complex steps.
- * This method has shown promising results, identifying viral proteins in as little as 20 minutes with high sensitivity, suggesting its potential for rapid clinical diagnosis and aiding in disease control.
Aim: The uptake pathway of liposomes into cells is mainly via endocytosis or membrane fusion; however, the relationship between the uptake pathway and the intracellular pharmacokinetics of the liposome components remains unclear. This study aimed at revealing the relationship by using cationic liposomes having similar physical properties and different uptake pathways.
Materials And Methods: We prepared cationic liposomes composed of amino acid-type lipids, K3C14 and K3C16, which have different uptake pathways by a hydration method, and fluorescently modified them by encapsulating FITC-dextran and surface conjugation with Alexa Fluor 488 (AF488).